The non-classical ArsR-family repressor PyeR (PA4354) modulates biofilm formation in Pseudomonas aeruginosa

Aogáin, Micheál Mac; Mooij, Marlies J.; McCarthy, Ronan R.; Plower, E.; Wang, Yi‐Ping; Tian, Zhe-Xian; Dobson, Alan D. W.; Morrissey, John P.; Adams, Claire; O’Gara, Fergal

doi:10.1099/mic.0.058636-0

Cited by 22 publications

(15 citation statements)

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“…MexT regulates the pyeRM-xenB operon, in which pyeR encodes an ArsR-family repressor, pyeM a major facilitator superfamily (MFS) transporter and xenB an uncharacterized xenobiotic reductase (37) (Fig. 2C).…”

Section: Links Between Antimicrobial Resistance and Hocl Susceptibilimentioning

confidence: 99%

“…For mexC and mexD, 2 transposon mutants of each gene were available and showed similar phenotypes, therefore only 1 mutant of each is displayed. (C) Gene arrangement of the pyeRM-xenB operon; PyeR autorepresses the pyeRM-xenB operon (37). Growth of PA14 WT and ∆pyeRM-xenB strains in the presence of 5.1 mM HOCl.…”

Section: Homology Modelling and Sequence Analysismentioning

confidence: 99%

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Response ofPseudomonas aeruginosato the innate immune system-derived oxidants hypochlorous acid and hypothiocyanous acid

Farrant

Spiga

Davies

et al. 2020

Preprint

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Pseudomonas aeruginosa is a significant nosocomial pathogen and associated with lung infections in cystic fibrosis (CF). Once established, P. aeruginosa infections persist and are rarely eradicated despite the host immune cells producing antimicrobial oxidants, including hypochlorous acid (HOCl) and hypothiocyanous acid (HOSCN). There is limited knowledge as to how P. aeruginosa senses, responds to, and survives attack from HOCl and HOSCN, and the contribution of such responses to its success as a CF pathogen. We investigated the P. aeruginosa response to these oxidants by screening 707 transposon mutants, with mutations in regulatory genes, for altered growth following HOCl exposure. We identified regulators involved in antibiotic resistance, methionine biosynthesis and catabolite repression, and PA14_07340, the homologue of the Escherichia coli HOCl-sensor RclR (30% identical), that were required for HOCl survival. We have shown that RclR (PA14_07340) protects specifically against HOCl and HOSCN stress, and responds to both oxidants by upregulating expression of a putative peroxiredoxin, rclX (PA14_07355). While there was specificity in the transcriptional response to HOCl (231 genes upregulated) and HOSCN (105 genes upregulated) there was considerable overlap, with 74 genes upregulated by both oxidants. These included genes encoding the type III secretion system (T3SS), sulphur and taurine transport, and

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Section: Links Between Antimicrobial Resistance and Hocl Susceptibilimentioning

confidence: 99%

Section: Homology Modelling and Sequence Analysismentioning

confidence: 99%

Response ofPseudomonas aeruginosato the innate immune system-derived oxidants hypochlorous acid and hypothiocyanous acid

Farrant

Spiga

Davies

et al. 2020

Preprint

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“…7, Additional file 1: Table S3). Additionally, several other genes which are not directly involved in biofilm formation but have been reported to mediate the process were also found to be expressed at a higher level in ATCC 27853 than in PAO1, such as Chaperone-usher pathway ( cup ) A (PA2128-PA2132, cupA1-A4 ) encoding genes which were found to be required for adhesion to inert surfaces [32, 33], the cbb3 -type cytochrome c oxidase cco2 gene cluster ( ccoN2O2Q2P2 , PA1555-PA1557) which has been shown to promote biofilm formation under hypoxia through NO induction and its effect on cell elongation [34], as well as pyeR (PA4354) that encodes a non-classical ArsR family member of transcriptional regulators modulating biofilm formation in P. aeruginosa [35] (Fig. 7, Additional file 1: Table S3).…”

Section: Resultsmentioning

confidence: 99%

Comparative genome and transcriptome analysis reveals distinctive surface characteristics and unique physiological potentials of Pseudomonas aeruginosa ATCC 27853

et al. 2017

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Background Pseudomonas aeruginosa ATCC 27853 was isolated from a hospital blood specimen in 1971 and has been widely used as a model strain to survey antibiotics susceptibilities, biofilm development, and metabolic activities of Pseudomonas spp.. Although four draft genomes of P. aeruginosa ATCC 27853 have been sequenced, the complete genome of this strain is still lacking, hindering a comprehensive understanding of its physiology and functional genome.ResultsHere we sequenced and assembled the complete genome of P. aeruginosa ATCC 27853 using the Pacific Biosciences SMRT (PacBio) technology and Illumina sequencing platform. We found that accessory genes of ATCC 27853 including prophages and genomic islands (GIs) mainly contribute to the difference between P. aeruginosa ATCC 27853 and other P. aeruginosa strains. Seven prophages were identified within the genome of P. aeruginosa ATCC 27853. Of the predicted 25 GIs, three contain genes that encode monoxoygenases, dioxygenases and hydrolases that could be involved in the metabolism of aromatic compounds. Surveying virulence-related genes revealed that a series of genes that encode the B-band O-antigen of LPS are lacking in ATCC 27853. Distinctive SNPs in genes of cellular adhesion proteins such as type IV pili and flagella biosynthesis were also observed in this strain. Colony morphology analysis confirmed an enhanced biofilm formation capability of ATCC 27853 on solid agar surface compared to Pseudomonas aeruginosa PAO1. We then performed transcriptome analysis of ATCC 27853 and PAO1 using RNA-seq and compared the expression of orthologous genes to understand the functional genome and the genomic details underlying the distinctive colony morphogenesis. These analyses revealed an increased expression of genes involved in cellular adhesion and biofilm maturation such as type IV pili, exopolysaccharide and electron transport chain components in ATCC 27853 compared with PAO1. In addition, distinctive expression profiles of the virulence genes lecA, lasB, quorum sensing regulators LasI/R, and the type I, III and VI secretion systems were observed in the two strains.ConclusionsThe complete genome sequence of P. aeruginosa ATCC 27853 reveals the comprehensive genetic background of the strain, and provides genetic basis for several interesting findings about the functions of surface associated proteins, prophages, and genomic islands. Comparative transcriptome analysis of P. aeruginosa ATCC 27853 and PAO1 revealed several classes of differentially expressed genes in the two strains, underlying the genetic and molecular details of several known and yet to be explored morphological and physiological potentials of P. aeruginosa ATCC 27853.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-017-3842-z) contains supplementary material, which is available to authorized users.

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“…For eight of these loci an increased transcript level is also observed in EV cells relative to WT cells (Table S3, Table 4). Among these loci are PA5471 gene encoding ArmZ, an inhibitor of MexZ which negatively regulates the mexXY operon encoding the multidrug efflux pump MexXY [58,59] and pyeR ( PA4354 ) encoding a transcriptional regulator of biofilm formation [60]. Additionally, Cluster 1 contains the arr ( PA2818 ) gene predicted to encode a phosphodiesterase whose substrate is cyclic di-guanosine monophosphate (c-di-GMP), a bacterial secondary messenger that regulates cell surface adhesiveness, virulence and biofilm formation [61].…”

Section: Resultsmentioning

confidence: 99%

Increased ParB level affects expression of stress response, adaptation and virulence operons and potentiates repression of promoters adjacent to the high affinity binding sitesparS3andparS4inPseudomonas aeruginosa

Kawałek

Glabski

Bartosik

et al. 2017

Preprint

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The non-classical ArsR-family repressor PyeR (PA4354) modulates biofilm formation in Pseudomonas aeruginosa

Cited by 22 publications

References 42 publications

Response ofPseudomonas aeruginosato the innate immune system-derived oxidants hypochlorous acid and hypothiocyanous acid

Response ofPseudomonas aeruginosato the innate immune system-derived oxidants hypochlorous acid and hypothiocyanous acid

Comparative genome and transcriptome analysis reveals distinctive surface characteristics and unique physiological potentials of Pseudomonas aeruginosa ATCC 27853

Increased ParB level affects expression of stress response, adaptation and virulence operons and potentiates repression of promoters adjacent to the high affinity binding sitesparS3andparS4inPseudomonas aeruginosa

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