The non-viral vectors and main methods of loading siRNA onto the titanium implants and their application

Chen, Liangrui; Bai, Mingxuan; Du, Ruiyu; Wang, Hao; Deng, Yi; Xiao, Anqi; Gan, Xueqi

doi:10.1080/09205063.2020.1793706

Cited by 5 publications

(2 citation statements)

References 75 publications

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“…Scientific research and clinical experience suggest that osseointegration is one of the most important factors to achieve good planting effect [ 170 ]. The implant osseointegration could be defined into multiple biological processes including protein adsorption on titanium, inflammatory cell adhesion/inflammatory response, additional relevant cells adhesion, and angiogenesis/osteogenesis [ 171 ].…”

Section: Therapeutic Applicationsmentioning

confidence: 99%

The current status of stimuli-responsive nanotechnologies on orthopedic titanium implant surfaces

Han,

Ma,

et al. 2023

J Nanobiotechnol

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With the continuous innovation and breakthrough of nanomedical technology, stimuli-responsive nanotechnology has been gradually applied to the surface modification of titanium implants to achieve brilliant antibacterial activity and promoted osteogenesis. Regarding to the different physiological and pathological microenvironment around implants before and after surgery, these surface nanomodifications are designed to respond to different stimuli and environmental changes in a timely, efficient, and specific way/manner. Here, we focus on the materials related to stimuli-responsive nanotechnology on titanium implant surface modification, including metals and their compounds, polymer materials and other materials. In addition, the mechanism of different response types is introduced according to different activation stimuli, including magnetic, electrical, photic, radio frequency and ultrasonic stimuli, pH and enzymatic stimuli (the internal stimuli). Meanwhile, the associated functions, potential applications and developing prospect were discussion.

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Section: Therapeutic Applicationsmentioning

confidence: 99%

The current status of stimuli-responsive nanotechnologies on orthopedic titanium implant surfaces

Han,

Ma,

et al. 2023

J Nanobiotechnol

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“…More importantly, the interaction between carrier system and implant biophysical cues was rarely explored, especially for further application in vivo practical conditions. Nevertheless, screening efficient osteoinductive gene/non-viral vectors [ 9 , 13 ], increasing the thickness of the coating [ 14 ] and changing loading strategies [ 15 ] can ameliorate the situation to a certain extent. Apart from this, micropattern topological structure modification (the roughness, width and depth) [ 16 ] has been documented as a useful physical technique, enabling an augmented specific surface area for enhanced DNA loading capacity [ 10 ] and inducing directional adhesion and migration of osteoblasts to accelerate the osseointegration process [ 17 ].…”

Section: Introductionmentioning

confidence: 99%

Fibrous topology promoted pBMP2-activated matrix on titanium implants boost osseointegration

He,

Wang,

Wang

et al. 2023

Regenerative Biomaterials

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Titanium (Ti) implants have been extensively used after surgical operations. Its surface bioactivity is of importance to facilitate integration with surrounding bone tissue, and ultimately ensure stability and long-term functionality of the implant. The plasmid DNA-activated matrix (DAM) coating on the surface could benefit osseointegration but is still trapped by poor transfection for further application, especially on the bone marrow mesenchymal stem cells (BMSCs) in vivo practical conditions. Herein, we constructed a DAM on the surface of fibrous-grained titanium (FG Ti) composed of phase-transition lysozyme (P) as adhesive, cationic arginine-rich lipid (RLS) as the transfection agent and plasmid DNA (pDNA) for bone morphology protein 2 (BMP2) expression. The cationic lipid RLS improved up to 30-fold higher transfection than that of commercial reagents (Lipofectamine 2000 and polyethyleneimine) on MSC. And importantly, Ti surface topology not only promotes the DAM to achieve high transfection efficiency (∼75.7% positive cells) on MSC due to the favorable combination but also reserves its contact induction effect for osteoblasts. Upon further exploration, the fibrous topology on FG Ti could boost pDNA uptake for gene transfection, and cell migration in MSC through cytoskeleton remodeling and induce contact guidance for enhanced osteointegration. At the same time, the cationic RLS together with adhesive P were both antibacterial, showing up to 90% inhibition rate against Escherichia coli and Staphylococcus aureus with reduced adherent microorganisms and disrupted bacteria. Finally, the FG Ti-P/pBMP2 implant achieved accelerated bone healing capacities through highly efficient gene delivery, aligned surface topological structure and increased antimicrobial properties in a rat femoral condylar defect model.

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Gene-activated titanium implants for gene delivery to enhance osseointegration

Wang

Zhang

et al. 2022

Biomaterials Advances

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The non-viral vectors and main methods of loading siRNA onto the titanium implants and their application

Cited by 5 publications

References 75 publications

The current status of stimuli-responsive nanotechnologies on orthopedic titanium implant surfaces

The current status of stimuli-responsive nanotechnologies on orthopedic titanium implant surfaces

Fibrous topology promoted pBMP2-activated matrix on titanium implants boost osseointegration

Gene-activated titanium implants for gene delivery to enhance osseointegration

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