The Nonlogarithmic Rate of Thermal Destruction of Spores of Bacillus coagulans12

Heat Resistance of Sporolactobacillus inulinus

“…Growth in Tomato Juice medium (Kitahara and Lai, 1967) was quite poor. This result was surprising in light of the variety of lactobacilli media containing tomato juice.…”

“…Cells were harvested from liquid media by centrifugation of 5860 x G for 20 min at 2°C. The pellets were resuspended in 100 ml of HCl at pH 2.0 (Kitahara and Lai, 1967) and recentrifuged. Cells were washed twice in 0.2M sodium phosphate buffer, pH'7.0 and resuspended in 100 ml sterile distilled water, prior to storage at 2°C.…”

“…Preliminary studies conducted on the effects of heating cell suspensions of sporolactobacilh previously grown in glucose-yeast extrac.t-geptone medium suggested a decimal reduction time at $10 C of approximately 1 min (Kitahara and Suzuki, 1963). Kitahara and Lai (1967) used this medium to optimize spore numbers and increase heat resistance of sporolac tobacilli. Calcium carbonate was added as a buffering agent; tomato serum was included as a source of manganese; meat extract was substituted for peptone; and ammonium sulfate was added.…”

“…From the limited data available it would appear that the DsOoG ranged from 1.9 min for o-methyl glucoside grown spores to 1.4 mm for inulin grown spores. Spores were rapidly killed as the temperature approached 90°C (Kitahara and Lai, 1967 Although there is a vast amount of information conceming heat resistance of Bacillus species in various substrates, there is a paucity of data on heat resistance of Sporolactobacillus inulinus. The aim of the present investigation was to examine the influence of media components on heat resistance of Sporolactobacillus and to determine decimal reduction times and z values.…”

The Occurrence of Bacillus coagulans with High Heat Resistance

“…Anderson, Esselen & Fellers (1949) and Knock, Lambrechts, Hunter & Riley (1959) demonstrated that the phosphate concentration in the heating medium, the p H and several other factors affect the heat resistance of the spores. Frank & Campbell (1957) showed that the rate of thermal destruction of the spores of B. coagulans can be nonlogarithmic:…”