The novel G-quadruplex-containing long non-coding RNA GSEC antagonizes DHX36 and modulates colon cancer cell migration

Matsumura, Kosuke; Kawasaki, Yuki; Miyamoto, Masaharu; Kamoshida, Yuki; Nakamura, Junya; Negishi, Lumi; Suda, Sakiko; Akiyama, Tetsu

doi:10.1038/onc.2016.282

Cited by 91 publications

(66 citation statements)

References 43 publications

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“…In addition, previous studies have shown that DHX9 plays an important role in maintaining gene stability and promotes the survival of tumor cells [44] [45] [46]. Recent proteomic studies also suggest elevated DHX9 expression levels in CRC, whereas DHX36 silence reduced CRC invasiveness and metastasis [26] [47]. These studies indicate that the helicase family may be also involved in the development of CRC.…”

Section: The Expression Profile Of Helicases Family Members In Crcmentioning

confidence: 88%

DNA sensors, crucial receptors to resist pathogens, are deregulated in colorectal cancer and associated with initiation and progression of the disease

He¹,

Liu²,

Lai³

et al. 2020

J. Cancer

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Background: DNA sensors are innate immune receptors that detect intracellular endogenous or exogenous DNA. They are critical to trigger immune response against DNA viral and intracellular bacterial infection, and are involved in inflammatory diseases and tumorigenesis. Recent accumulating evidences indicated that DNA sensors are also crucial for controlling the development of colorectal cancer (CRC). However, a systematic study on the expression profile of DNA sensors in CRC and their clinical significance are still lacking. Methods: We investigated the expression profile of DNA sensors in CRC and their clinical significance by taking advantage of clinical CRC samples, mouse AOM/DSS treatment model, and Oncomine ® bioinformatics platform. Results: Our study identified that the expression of DNA sensors, including AIM2, DAI, as well as inflammasome molecules ASC/IL-18, TLR9 and adaptor MyD88, and DDX60 decreased in human CRC, whereas the expression of DHX9, DHX36, and DDX41 significantly increased. Among them, the expression of AIM2/ASC/IL-18, MyD88, DAI, DHX36, and DDX60 were associated with cancer stages. In addition, we also performed correlation analysis between DNA sensors and their main signaling molecules to explore the possible mechanisms. The results showed that there were positive correlations between AIM2 and ASC/IL-18, DHX9 and MAVS, and TLR9 and MyD88 expression. In addition, the gene expression patterns of some DNA sensors were confirmed by Western-blot analysis. Conclusions: Our study revealed that the expression of multiple DNA sensors was deregulated in CRC and might be involved in tumor development. More importantly, the study identified that, among all these DNA sensors, AIM2, DAI, and DDX60 could be potentially critical for diagnosis, prognosis, and therapy of CRC and deserve further investigation.

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Section: The Expression Profile Of Helicases Family Members In Crcmentioning

confidence: 88%

DNA sensors, crucial receptors to resist pathogens, are deregulated in colorectal cancer and associated with initiation and progression of the disease

He¹,

Liu²,

Lai³

et al. 2020

J. Cancer

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“…Recent work from Matsumura et al . demonstrated the biological relevance of a G4 in the lncRNA GSEC (G-quadruplex-forming sequence containing lncRNA) 167 . This cytoplasmic lncRNA is implicated in the development of colorectal cancer where it stimulates migration of colon cancer cells, and depletion of GSEC results in the reduction of cancer cell mobility.…”

Section: Emerging Roles Of Rna G4s In Biology Of Non-coding Rnasmentioning

confidence: 99%

“…DHX36 recognizes a G4 in GSEC , and mutations of key guanines that affect G4 folding also reduce their interaction. Functionally, DHX36 inhibits mobility of colon cancer cells and GSEC antagonizes its effect by acting as a molecular decoy to suppress DHX36 function 167 .…”

Section: Emerging Roles Of Rna G4s In Biology Of Non-coding Rnasmentioning

confidence: 99%

RNA G-Quadruplexes in Biology: Principles and Molecular Mechanisms

Fay

Lyons

Ivanov

2017

Journal of Molecular Biology

360

306

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G-quadruplexes are extremely stable DNA or RNA secondary structures formed by sequences rich in guanine. These structures are implicated in many essential cellular processes and the number of biological functions attributed to them continues to grow. While DNA G-quadruplexes are well understood on structural and to some extent on functional levels, RNA G-quadruplexes and their functions have received less attention. The presence of bona fide RNA G-quadruplexes in cells has long been a matter of debate. The development of G-quadruplex-specific antibodies and ligands hinted on their presence in vivo but recent advances in RNA sequencing coupled with chemical footprinting suggested the opposite. In this review, we will critically discuss the biology of RNA G-quadruplexes focusing on the molecular mechanisms underlying their proposed functions.

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“…as in long-noncoding RNA (Matsumura et al 2017), suggesting roles of rG4 in the noncoding RNA transcriptome. Herein, we describe recent methodological advances to detect rG4s in the transcriptome.…”

Section: Introductionmentioning

confidence: 99%

Detecting RNA G-Quadruplexes (rG4s) in the Transcriptome

Kwok

Marsico

Balasubramanian

2018

Cold Spring Harb Perspect Biol

106

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RNA G-quadruplex (rG4) secondary structures are proposed to play key roles in fundamental biological processes that include the modulation of transcriptional, co-transcriptional, and posttranscriptional events. Recent methodological developments that include predictive algorithms and structure-based sequencing have enabled the detection and mapping of rG4 structures on a transcriptome-wide scale at high sensitivity and resolution. The data generated by these studies provide valuable insights into the potentially diverse roles of rG4s in biology and open up a number of mechanistic hypotheses. Herein we highlight these methodologies and discuss the associated findings in relation to rG4-related biological mechanisms.

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The novel G-quadruplex-containing long non-coding RNA GSEC antagonizes DHX36 and modulates colon cancer cell migration

Cited by 91 publications

References 43 publications

DNA sensors, crucial receptors to resist pathogens, are deregulated in colorectal cancer and associated with initiation and progression of the disease

DNA sensors, crucial receptors to resist pathogens, are deregulated in colorectal cancer and associated with initiation and progression of the disease

RNA G-Quadruplexes in Biology: Principles and Molecular Mechanisms

Detecting RNA G-Quadruplexes (rG4s) in the Transcriptome

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