The Nuclear Envelope in Freeze-Etching

Abstract

ISBN 3-540-05538-X Springer·Verlag Berlin Heidelberg New York ISBN 0-387-05538-X Springer·Verlag New York Heidelberg Berlin Das Werk ist urheberrechtlich geschiitzt. Die dadurch begriindeten Rechte, insbesondere die der tibersetzung des Nachdruckes, der Entnahme van Abbildungen, der Funksendung, der Wiedergabe aul photomerhaniscbem oder ahnlichem 'Vege und der Speicherung in Datenverarbeitungsanlagen, bleiben, auch bei nUT auszugsweiser Verwertung, vorbehaltenBei Vervielliiltigungen liir gewerbJiche Zwecke ist gemiiB § 54 UrhG eine Vergiitung an den Verlag zu zahlen, deren Hohe mit dem Verlag zu vereinbaren ist

A simple technique for the rapid preparation of freeze‐fracture replicas of plant nuclei

“…Although corresponding calculations made from thin-sectioned material gave higher values, possibly as a result of slight shrinkage of nuclei during the dehydration stage of processing (cf. Kartenbeck et al, 1971) this difference is not statistically significant (P > 0.01). Thus, pore frequency data obtained by the three methods are comparable.…”

Nuclear pore flow rate of ribosomal RNA and chain growth rate of its precursor during oogenesis of Xenopus laevis

“…Increase of the oocyte diameter (--) and of the ooplastions tangential to nuclear envelope (cf. Franke and Scheer, 1970a,b;Kartenbeck et al, 1971). All three different electron microscopic preparative methods gave nearly identical data (for a detailed methodical discussion see Kartenbeck et al, 1971), thus allowing the conclusion that the quantitative structural data of the nuclear envelope are unaffected by methodical artifacts.…”

Ion channels in murine nuclei during early development and in fully differentiated adult cells

“…Shrinkage during fixation would cause a systematic error in these numbers. Freeze etching is the most quantitative method to measure density (Maul, 1977), and Kartenbeck et al (1971) compared the values they obtained in rat hepatocytes using both transmission EM and freeze etching. They found that the density of pores in thin section is larger than the density of pore in freeze etch.…”