The nucleotide sequence of lysine tRNA<sub>2</sub>from Drosophila

Silverman, Sanford J.; Gillam, I. C.; Tener, G. M.; Söll, Dieter

doi:10.1093/nar/6.2.435

Cited by 24 publications

(5 citation statements)

References 19 publications

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“…Sequence Analysis of Glutamate tRNA. The nucleotide sequence was elucidated by using both uniformly in vivo labeled radioactive tRNA and nonradioactive tRNA post-labeled in vi_tro (7,8).…”

Section: Introductionmentioning

confidence: 99%

The nucleotide sequence of the major glutamate transfer RNA from Schizosaccharomyces pombe

et al. 1979

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The nucleotide sequence of glutamate tRNA1 from Schizosaccharomyces pombe was determined to be pU-C-C-G-U-U-G-U-m1G-G-U-C-C-A-A-C-G-G-C-D-A-G-G-A-U-U-C-G-U-C-G-C-U-U-U*-C-A-C-C-G-A-C-G-G-G-A-G-m5C-G-G-G-G-T-psi-C-G-A-C-U-C-C-C-C-G-C-A-A-C-G-G-A-G-C-C-AOH. The sequence differs markedly from that of S. cerevisiae tRNAGlu. S. pombe glutamate tRNA1 can be aminoacylated by the homologous glutaminyl-tRNA synthetase as well as by the corresponding enzyme from S. cerevisiae.

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“…Sequence Analysis of Glutamate tRNA. The nucleotide sequence was elucidated by using both uniformly in vivo labeled radioactive tRNA and nonradioactive tRNA post-labeled in vi_tro (7,8).…”

Section: Introductionmentioning

confidence: 99%

The nucleotide sequence of the major glutamate transfer RNA from Schizosaccharomyces pombe

et al. 1979

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“…Covalently closed circular DNA of plasmids pCIT12 (6) (16) and purified by twodimensional polyacrylamide gel electrophoresis (17).…”

Section: Methodsmentioning

confidence: 99%

Specific transcription of eukaryotic tRNA genes in Xenopus germinal vesicle extracts.

Schmidt¹,

Mao²,

Silverman³

et al. 1978

Proc. Natl. Acad. Sci. U.S.A.

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Cloned tRNA genes from Drosophila and from yeast have been transcribed faithfully in extracts prepared from Xenopus germinal vesicles. The newly formed RNA is composed of precursor tRNAs (of 5S RNA size and of tRNAs. The plasmid pCIT12 carries genes for Drosophila tRNALYS, tRNAg, and tRNA1sn. Nucleotide analysis of one RNA species transcribed from pCIT12 DNA showed it to be identical to Drosophila tRNALYS; it even contained some of the modified nucleotides expected for this tRNA. This RNA species is formed in the germinal vesicle extract via a larger precursor tRNA molecule that does not contain nucleotide modifications. This simple transcription system should aid studies aimed at defining the regulatory DNA regions responsible for eukaryotic gene transcription. In addition, it may provide tRNA precursors that are neeiled for detailed investigations of eukaryotic tRNA biosynthesis. Our understanding of the process of RNA biosynthesis in eukaryotic cells and of its regulation is limited (1), due in part to the lack of recognized mutants affecting tRNA biosynthesis, even in genetically well-studied eukaryotic organisms such as yeast and Drosophila. The development of techniques for molecular cloning of DNA (reviewed in ref.2) and for rapid determination of nucleotide sequences (3-5) has given some insight into the organization of tRNA genes in the genome (6) and allowed elucidation of the actual DNA sequence of some tRNA genes (7,8). However, these findings have not provided an experimental strategy for defining the detailed sequence of enzymatic steps leading to mature tRNA, nor have they shed light on the importance of the intervening sequences found in some tRNA genes (7,8). But it is clear that novel enzymes must be involved to exclude such nucleotide sequences from the mature tRNA (9).The availability of tRNA genes emphasized the need for routine tests of their biological function. In particular, an in vitro transcription system is desirable in which cloned eukaryotic tRNA genes can be easily transcribed. This should help to define the presumptive sites of initiation and termination of transcription and also to make available precursor tRNAs for studies of their enzymatic conversion to mature tRNA. The Xenopus oocyte is a promising system for such studies. DNA injected into the oocyte nucleus can be transcribed in vivo (10, 11). Cloned Xenopus genes for 5S RNA (12) and for the initiator tRNA (13) have been faithfully transcribed into their mature products in this fashion. Brown and his colleagues (14) have greatly simplified this procedure by their demonstration that cloned Xenopus 5S DNA can be efficiently transcribed in vitro in an extract prepared from germinal vesicles.In this paper we show that cloned tRNA genes from Drosophila and from yeast can be faithfully transcribed in extracts from Xenopus germinal vesicles. The major products of transcription are tRNA precursors which can be processed to mature tRNA in the germinal vesicle extract. (1500), and tRNALYS(1900). For hybridization 32P-5'-end...

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“…As part of a study on lysine tRNAs and their genes we report here the nucleotide sequence of tRNALys 5. The procedures used have been largely described elsewhere. tRNALy was purified by repeated chromatography analogous to that for Lys Val described for tRNA2Y (11) and tRNA3b (16).…”

Section: ~~~~~~~~~~~~~~~~~~5mentioning

confidence: 99%

“…has been sequenced (11) as have a number of its genes (12,13) and transcription of recombinant plasmids containing normal or rearranged tRNALy genes has been examined (14). Genes for tRNALys have also…”

Section: Introductionmentioning

confidence: 99%

The structure of tRNA5^LysfromDrosophila melanogaster

1982

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The nucleotide sequence of Drosophila melanogaster tRNA 5 Lys is pGCCCGGAUAm2GCUCAGDCGGDAGAGCA psi psi GGACUsU*UUt6A*A psi CCAAGGm7GDm5CCAGGGTm psi CAm1AGUCCCUGUUCGGGCGCCA. The sU* is probably 5-methylcarboxymethyl-2-thiouridine and t6A* is a mixture of modified derivatives of t6A including t6A itself and a component sensitive to treatment with cyanogen bromide. This tRNA 5 Lys is 95% homologous to the rabbit liver tRNA 5 Lys.

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The nucleotide sequence of lysine tRNA₂from Drosophila

Abstract: The nucleotide sequence of Drosophila meZanogaster lysine tRNA2 was determined to be:

Cited by 24 publications

References 19 publications

The nucleotide sequence of the major glutamate transfer RNA from Schizosaccharomyces pombe

The nucleotide sequence of the major glutamate transfer RNA from Schizosaccharomyces pombe

Specific transcription of eukaryotic tRNA genes in Xenopus germinal vesicle extracts.

The structure of tRNA5^LysfromDrosophila melanogaster

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The nucleotide sequence of lysine tRNA2from Drosophila

Abstract: The nucleotide sequence of Drosophila meZanogaster lysine tRNA2 was determined to be:

Cited by 24 publications

References 19 publications

The nucleotide sequence of the major glutamate transfer RNA from Schizosaccharomyces pombe

The nucleotide sequence of the major glutamate transfer RNA from Schizosaccharomyces pombe

Specific transcription of eukaryotic tRNA genes in Xenopus germinal vesicle extracts.

The structure of tRNA5LysfromDrosophila melanogaster

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The nucleotide sequence of lysine tRNA₂from Drosophila

The structure of tRNA5^LysfromDrosophila melanogaster