The nucleotide sequence of the coding region of tobacco etch virus genomic RNA: Evidence for the synthesis of a single polyprotein

Analysis of in vitro Translation of Bean Yellow Mosaic Virus RNA: Inhibition of Proteolytic Processing by Antiserum to the 49K Nuclear Inclusion Protein

“…Sequence data for the genomes of two potyviruses indicate that potyviruses may have a linkage of protease and replicase proteins similar to that of picornaviruses and comoviruses (Allison et al, 1986;Domier et al, 1987). The genomic organization of BYMV-PV-2 RNA resolved by in vitro translation product analysis reported here is consistent with that known for TEV and TVMV.…”

“…1, 2), in the B Y M V -P V -2 R N A translations done without D D T . The genomic organization proposed for B Y M V -P V -2 is similar to that resolved by sequencing the genomes of two other potyviruses, T V M V and T E V (Allison et al, 1986). The conversion of high Mr translation products into mature virus-specified proteins upon incubation with D T T in the translation m e d i u m implicated post-translational proteolytic processing of the B Y M V -P V -2 polyproteins.…”

Sequence polymorphism in the 5'NTR and in the P1 coding region of potato virus Y genomic RNA

“…The flexuous particles contain a single-stranded messenger RNA of about 10000 nucleotides with a viral protein covalently linked to the 5' end and a poly(A) tail at the 3' end (for a review see Riechmann et al, 1992). The complete nucleotide sequence of several members of the group is already known (Allison et al, 1986;Domier et al, 1986;Robaglia et al, 1989;Lain et al, 1989;Maiss et al, 1989;Teycheney et al, 1989;Johansen et al, 1991 ; * Author for correspondence. Fax +33 1 30 83 31 95. e-mail robaglia@oxygene.versailles.inra.fr Nicolas & Lalibertr, 1992;Yeh et al, 1992;Jayaram et al, 1992;Vance et al, 1992;Thole et al, 1993;Palkovics et al, 1993).…”

The nucleotide sequence of potato virus A genomic RNA and its sequence similarities with other potyviruses

“…The complete nucleotide sequence of seven different potyviruses has been documented: tobacco etch virus (TEV) (Allison et al, 1986), tobacco vein mottling virus (TVMV) (Domier et al, 1986), plum pox virus (PPV) (Maiss et al, 1989;La~n et al, 1989), the necrotic strain of potato virus Y (PVY N) (Robaglia et al, 1989), pepper mottle virus (PepMoV) (Vance et al, 1992), turnip mosaic potyvirus (TuMV) (Nicolas & Lalibert& 1992) and pea seed-borne mosaic virus (Johansen et al, 1991). The sequence analysis of potyviruses and in vitro translation studies of potyvirus genomic RNAs have revealed a single open reading frame (ORF) encoding a large polyprotein.…”