The Nucleotide Sequence of the Pieris brassicae Granulosis Virus Granulin Gene

SUMMARYThe genomes of four closely related variants of Artogeia rapae granulosis virus, isolated in England and New Zealand, have been mapped for the restriction endonucleases EcoRI, BamHI, HindlII, KpnI, XhoI, PstI, BglI, and Sinai by secondary digestion of isolated restriction fragments. A total of 69, 70 or 71 fragments were unambiguously mapped for these isolates. A modified system of fragment lettering was adopted so that, for each enzyme, the same letter could be used for colinear fragments from each isolate despite loss or gain of restriction sites. The sizes of the viral DNAs varied slightly, but were all about 110 kbp. The zero point for the maps was taken as the start of the granulin gene which was orientated in the same direction as the polyhedrin gene on the map of Autographa californica multiple nucleocapsid nuclear polyhedrosis virus DNA. Differences between the four variants are discussed in relation to their insect host range.

supporting

confidence: 70%

Physical Maps of the Genomes of Four Variants of Artogeia rapae Granulosis Virus

Smith

Crook

1988

“…This consensus sequence is also present in both of the pl0 genes which have been analysed [AcMNPV, Kuzio et al, 1984;Liibbert & Doerfter, 1984: Orgyia pseudotsugata (Op) MNPV, Leisy et al, 1986c]. The subgroup B baculoviruses (granulosis viruses; GVs) also have the same consensus sequence upstream of the granulin gene, which encodes an occlusion body protein of similar function to polyhedrin (Akiyoshi et al, 1985;Chakerian et al, 1985). The polyhedrin mRNA start sites have been accurately determined for AcMNPV (Howard et al, 1986) and OpMNPV (Leisy et al, 1986a).…”

Section: Short Communicationmentioning

confidence: 98%

Analysis of Transcription Initiation in the Panolis flammea Nuclear Polyhedrosis Virus Polyhedrin Gene

Oakey¹,

Cameron²,

Davis³

et al. 1989

SUMMARYThe nucleotide sequence of the polyhedrin gene of Pano/is flammea multiple nucleocapsid polyhedrosis virus (PfMNPV) has been determined. The coding sequences of this gene shared 82% similarity at the DNA level and 88% similarity at the protein level with the polyhedrin gene from Autographa califomica (Ac) MNPV. A single nucleotide deviation from the consensus transcription initiation sequence for baculovirus very late genes was identified in the PfMNPV polyhedrin gene. RNA was prepared from Mamestra brassicae larvae infected with PfMNPV and compared with RNA harvested at 24 h post-infection from AcMNPV-infected Spodopterafrugiperda

“…4). This sequence appears to be conserved throughout the Baculoviridae: related sequences have been described upstream of granulin genes (Akiyoshi et al, 1985;Chakerian et al, 1985), AcMNPV 10K (Kuzio et al, 1984) and OpMNPV 10K (D. Leisy et al, unpublished observations) protein genes and the polyhedrin genes of AcMNPV (Hooft van Iddekinge et al, 1983), Bombyx mori NPV (BmNPV) (Iatrou et al, 1985) and OpMNPV (D. Leisy et al, unpublished observations). All hypertranscribed baculovirus genes examined (polyhedrin and 10K), have mRNA initiation sites at or very near this conserved sequence (Hooft van Iddekinge et al, 1983;Kuzio et al, 1984;D.…”

Section: Genome Map and Location Of The Polyhedrin Genementioning

confidence: 99%

Location and Nucleotide Sequence of the Orgyia pseudotsugata Single Nucleocapsid Nuclear Polyhedrosis Virus Polyhedrin Gene

Leisy

Nesson

Pearson

et al. 1986

SUMMARYA restriction endonuclease map was determined for the Orgyia pseudotsugata single nucleocapsid nuclear polyhedrosis virus (SNPV) genome. The order of the fragments generated by the enzymes BglII, BamHI and XbaI was analysed using double digestion of the total genome and digestion of isolated restriction fragments. The location of the polyhedrin gene was then determined using a cloned polyhedrin gene from the O. pseudotsugata multiple nucleocapsid NPV (MNPV) as a hybridization probe. A fragment containing this gene was cloned, mapped, subcloned and the nucleotide sequence of a 1.3 kb fragment was determined which contained the entire polyhedrin reading frame and some flanking sequences. This gene demonstrated 76% nucleotide sequence homology and 87% amino acid sequence homology to the Autographa californica MNPV polyhedrin sequence. A probable regulatory element was identified which is common to the 5' flanking region of all hypertranscribed late genes (polyhedrin and 10K proteins) which have been examined in baculoviruses.