The nucleotide sequence of the sheep MHC class II DNA gene

Transcription of the unique ruminant class II major histocompatibility complex-DYA andDIB genes in dendritic cells

“…By analogy, it is therefore possible that DYA and DIB genes are transcribed by bovine thymic epithelial cells. However, DNA and DOB genes have also been described in cattle and sheep [13,24,25], so it is likely that an expressed bovine DY/DI dimer is functionally distinct from DO. In addition, whereas DO molecules are found in human B cells, we failed to detect DY or DI transcripts in bovine PBM, 20% of which normally comprise surface IgM + B cells.…”

Cattle MHC genes DOA and DOB: sequence polymorphisms and assignments to the class IIb region

“…In order to study the polymorphisms of the cattle DO genes, primers were derived from the sheep homologues, OLA‐DNA and OLA‐DOB (Wright et al. , 1995, 1996; Heß et al.…”

“…1; GenBank accession numbers AF143237 and AF143238). At the predicted amino acid level, the nucleotide substitution corresponded to a change in the respective residue, 38 K → R, according to the numbering system of Wright et al . (1995).…”

“…The class IIa region contains the human homologues of the functionally expressed DQ and DR genes, while the class IIb region contains nonclassical MHC genes such as DYA , DIB , DOB , DNA , DMA, DMB, TAP2 , LMP2 and LMP7 Heß et al , 1999). Whereas variability of the class IIa genes is well documented, only limited information at present is available on allelic variants of the genes in the class IIb region Russell et al , 1997; http://www2.ri.bbsrc.ac.uk/bola/).In order to study the polymorphisms of the cattle DO genes, primers were derived from the sheep homologues, OLA-DNA and OLA-DOB (Wright et al , 1995(Wright et al , , 1996Heß et al , 1999). Following the Nomen-clature for Factors of the HLA System (Bodmer et al , 1999), we now refer to the cattle DNA sequence as BoLA-DOA .…”

“…In order to study the polymorphisms of the cattle DO genes, primers were derived from the sheep homologues, OLA-DNA and OLA-DOB (Wright et al , 1995(Wright et al , , 1996Heß et al , 1999). Following the Nomen-clature for Factors of the HLA System (Bodmer et al , 1999), we now refer to the cattle DNA sequence as BoLA-DOA .…”