The O:34-antigen lipopolysaccharide as an adhesin in Aeromonas hydrophila

Merino, Susana; Rubires, Xavier; Aguilar, Alicia; Tomás, Juan M.

doi:10.1016/0378-1097(96)00068-7

Cited by 40 publications

(55 citation statements)

References 7 publications

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“…4). The O-antigen LPS of A. hydrophila O:34 strains has been found to play an important role in adhesion to HEp-2 cells (31). However, we could not observe a similar inhibitory effect on the adhesion of A. hydrophila PPD134/91 (O:18) to EPC cells.…”

Section: Resultscontrasting

confidence: 87%

“…Both the O-antigen polysaccharide and the capsule are composed of repeating oligosaccharide units (44). They act as prominent antigens and play important roles in the pathogenicity of many bacterial pathogens, such as protecting bacterial cells from complement-mediated serum killing (20,30), acting as adhesion factors (31), protecting the bacteria from the effects of desiccation (38), and aiding survival in phagocytes (56). The serogrouping of bacterial strains within a genus is determined by the structural variability of surface polysaccharides.…”

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confidence: 99%

“…The genus Aeromonas has been classified into 96 serogroups (50,60), and a role for surface polysaccharides in the pathogenicity of certain A. hydrophila strains has been proposed. For example, the O-antigen lipopolysaccharide (LPS) of A. hydrophila O:34 strains has been found to play an important role in adhesion to HEp-2 cells (31). The O-polysaccharide from one virulent strain of A. hydrophila has been found to contain L-rhamnose and D-glucosamine and to have a backbone structure identical to that of the O-polysaccharide from Aeromonas salmonicida (53).…”

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NovelAeromonas hydrophilaPPD134/91 Genes Involved in O-Antigen and Capsule Biosynthesis

Zhang¹,

Arakawa

Leung

2002

Infect Immun

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The sequences of the O-antigen and capsule gene clusters of the virulent Aeromonas hydrophila strain PPD134/91 were determined. The O-antigen gene cluster is 17,296 bp long and comprises 17 genes. Seven pathway genes for the synthesis of rhamnose and mannose, six transferase genes, one O unit flippase gene, and one O-antigen chain length determinant gene were identified by amino acid sequence similarity. PCR and Southern blot analysis were performed to survey the distribution of these 17 genes among 11 A. hydrophila strains of different serotypes. A. hydrophila PPD134/91 might belong to serotype O:18, as represented by JCM3980; it contained all the same O-antigen genes as JCM3980 (97 to 100% similarity at the DNA and amino acid levels). The capsule gene cluster of A. hydrophila PPD134/91 is 17,562 bp long and includes 13 genes, which were assembled into three distinct regions similar to those of the group II capsule gene cluster of Escherichia coli and other bacteria. Regions I and III contained four and two capsule transport genes, respectively. Region II had five genes which were highly similar to capsule synthesis pathway genes found in other bacteria. Both the purified O-antigen and capsular polysaccharides increased the ability of the avirulent A. hydrophila strain PPD35/85 to survive in naïve tilapia serum. However, the purified surface polysaccharides had no inhibitory effect on the adhesion of A. hydrophila PPD134/91 to carp epithelial cells.Surface polysaccharides, such as O-antigen and capsule, are important bacterial cell surface components. The O-antigen polysaccharide is covalently ligated to the lipid A-core complex and extends outward from the cell surface. The capsule is an extracellular polysaccharide enclosing the bacterium while remaining attached to the cell. Both the O-antigen polysaccharide and the capsule are composed of repeating oligosaccharide units (44). They act as prominent antigens and play important roles in the pathogenicity of many bacterial pathogens, such as protecting bacterial cells from complement-mediated serum killing (20, 30), acting as adhesion factors (31), protecting the bacteria from the effects of desiccation (38), and aiding survival in phagocytes (56). The serogrouping of bacterial strains within a genus is determined by the structural variability of surface polysaccharides. For example, Escherichia coli strains are divided into more than 160 serogroups based on the different surface polysaccharides (67). Klebsiella species have been classified into 72 serogroups based on the structural variability of their capsular polysaccharides (39).Aeromonas hydrophila is an important pathogen of a wide variety of aquatic and terrestrial animals, especially fish (4). In fish, it causes hemorrhagic septicemia, which often results in high mortalities in commercial aquaculture. Some strains of A. hydrophila are also reported to cause infections in humans. The clinical symptoms include septicemia (17), meningitis (25), peritonitis (35), pneumonia (32), myonecrosis (34), and diarrh...

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Section: Resultscontrasting

confidence: 87%

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confidence: 99%

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confidence: 99%

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NovelAeromonas hydrophilaPPD134/91 Genes Involved in O-Antigen and Capsule Biosynthesis

Zhang¹,

Arakawa

Leung

2002

Infect Immun

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“…Our data relating to the banding position of the fi sh and diarrhoeal isolates showed similar results. A. jandaei produced diffused fast migrating bands, which may carry a lipid A core oligosaccharide fractions 8 . Our clinical isolates also produced diffusible bands (lane 1 and 4) with a molecular weight of 38 kDa.…”

Section: Resultsmentioning

confidence: 99%

“…They play an important role in pathogenecity of many bacterial pathogens 8 . It activates the cellular immunity and induces a cytokine mediated systemic infl ammatory response in the host 9 .…”

Section: P Lakshmanaperumalsamy Introductionmentioning

confidence: 99%

Typing of Aeromonas hydrophila of fish and human diarrhoeal origin by outer membrane proteins and lipopolysaccharides

et al. 2007

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AcuC, a histone deacetylase, contributes to the pathogenicity of Aeromonas hydrophila

et al. 2017

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The interactions of pathogens and phagocytes are complex. Our study demonstrated that Aeromonas hydrophila B11 can survive in the macrophagocytes of Tilapia mossambica. To explore the regulatory processes of A. hydrophila survival in the macrophagocytes, we used the mini‐Tn10 transposon mutagenesis system to build a mutant library by mixing Escherichia coli Sm10 (pLOFKm) and A. hydrophila B11. In total, 102 mutant colonies were detected, and 11 of them showed reduced survival in macrophagocytes. The mutant with the most severe phenotype, AM73, was chosen for further research. The ORF interrupted by mini‐Tn10 in AM73 was approximately 960 bp and was deposited in GenBank with the accession number SRP049226. The 319 amino acid protein encoded by the ORF showed a high degree of identity (89%) with proteins in the histone deacetylase/AcuC/AphA family of A. hydrophila subsp. hydrophila ATCC7966. A strain (AC73) in which the acuC mutation was complemented was constructed by generating the recombinant expression plasmid pACYC184‐acuC and introducing it into the AM73 mutant strain. Our experiments revealed that strain AM73 was deficient in biofilm formation, adhesion, survival in macrophagocytes, and virulence compared with A. hydrophila B11, and all of these biological properties were improved in strain AC73. The expression of 10 significant virulence genes was significantly inhibited in strain AM73. The results indicated that AcuC was an important regulatory protein contributing to the pathogenicity of A. hydrophila.

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The O:34-antigen lipopolysaccharide as an adhesin in Aeromonas hydrophila

Cited by 40 publications

References 7 publications

NovelAeromonas hydrophilaPPD134/91 Genes Involved in O-Antigen and Capsule Biosynthesis

NovelAeromonas hydrophilaPPD134/91 Genes Involved in O-Antigen and Capsule Biosynthesis

Typing of Aeromonas hydrophila of fish and human diarrhoeal origin by outer membrane proteins and lipopolysaccharides

AcuC, a histone deacetylase, contributes to the pathogenicity of Aeromonas hydrophila

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