The Occurrence of Electron Spin Resonance Signals in Bone Grafts Sterilized With High Voltage Electron Beams

Slager, Ursula T.; Zucker, Melvin J.

doi:10.1097/00006534-196210000-00050

Cited by 17 publications

(2 citation statements)

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“…Its usefulness for bone tissue characterization comes from the fact, that in the biominerals containing hydroxyapatite, such as tooth enamel or bone, irradiated with ionizing radiation, characteristic paramagnetic centers are induced. 24 They can be detected by EPR with a very high sensitivity, are extremely stable in time and their intensity responds in a controlled manner to the dosage of irradiation, which makes it useful for bone dating, dosimetry in tissues containing bone hydroxyapatites and detecting the remodeling of irradiated bone grafts in experimental systems. 26 , 28 …”

Section: Discussionmentioning

confidence: 99%

Synthetic Calcite as a Scaffold for Osteoinductive Bone Substitutes

et al. 2015

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Although a wide variety of biomaterials have been already proposed for use in bone tissue engineering, there is still need for man-made materials, which would combine support for osteogenesis with simplicity desirable for upscaling and costs reduction. In this study we have shown that synthetic calcite may serve as a scaffold for human osteoblasts transplantation. A simple dynamic system allows uniform and effective cell distribution. Cell viability and osteogenic phenotype were confirmed by XTT assay, alkaline phosphatase activity and selected osteoblast-specific genes expression. Extracellular matrix deposited by cells improved elasticity and made the whole system similar to the flexible composite material rather than to the brittle ceramic implants. It was revealed in the compression tests and also by the improved samples handling. Subcutaneous implantation of the cell-seeded calcite scaffolds to immunodeficient mice resulted in mineralized bone formation, which was confirmed histologically and by EPR analysis. The latter we propose as a method supplementary to histological analysis, for bone regeneration investigations. It specifically confirms the presence of bone mineral with a unique sensitivity and using bulk samples, which eliminates the risk of missing the material in the preparation. Our study resulted in development of a new osteogenic tissue engineered product based on man-made calcite.

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Section: Discussionmentioning

confidence: 99%

Synthetic Calcite as a Scaffold for Osteoinductive Bone Substitutes

et al. 2015

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“…The osteoinductive capabilities of irradiated bone decrease at a dose of 10 kGy and disappear completely at values over 25 kGy [27,152,172,179,194,195]. In addition, the free radicals created by irradiation are possibly mutagenic [97,161].…”

Section: Sterilization Of Bonementioning

confidence: 99%

Bone transplantation

Schweiberer¹,

St�tzle

Mandelkow

1989

Arch Orthop Trauma Surg

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According to one principle of surgery, the transplantation of vital tissue is the best method of reconstructing a defect. Because of absent immunologic reactions, high osteogenic potency, and preserved stability, transplantation of autogenous bone shows the best results. Necrosis of transplanted bone, leading inevitably to absorption and remodeling of the graft, can be avoided if microsurgically vascularized autogenous bone segments are transferred. Disadvantages are the low availability and the necessity of additional operations. As an alternative, deep-frozen allogeneic bone is used. However, this kind of bone shows delayed incorporation based on cellular and humoral immune reactions, and it is also installed into the host bed after overcoming the immune barrier. The risk of microbiological contamination or transmission of unrecognized germs such as HIV is a cause of great expense in bone banking techniques. If one succeeds in reducing (a) the immunologic defense reaction and (b) the risk of infection by sterilization or disinfection without damaging the osteoinductive proteins of bone matrix, the rate of complications can be lowered. Demineralized bone matrix can be used if biomechanical stability is not required. Its ability to induce osteogenesis without a major immune reaction or the risk of transmitting diseases justifies its clinical application. Further intensive research in these areas is unavoidable.

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Excitation Transfer in Bone

Hernández¹

1974

Arch Surg

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The Occurrence of Electron Spin Resonance Signals in Bone Grafts Sterilized With High Voltage Electron Beams

Cited by 17 publications

References 0 publications

Synthetic Calcite as a Scaffold for Osteoinductive Bone Substitutes

Synthetic Calcite as a Scaffold for Osteoinductive Bone Substitutes

Bone transplantation

Excitation Transfer in Bone

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