The occurrence of serotonin-containing cells in the esophageal epithelium of the bullfrog Rana catesbiana

Abstract

Fluorescence histochemical examination of biogenic amines of the frog esophageal mucosa revealed that a serotonin-like monoamine exhibiting an yellow fluorescence was present in a certain type of cells. The new type of cells was specifically stained by the immunohistochemical method using anti-serotonin antiserum. From these observations, it is suggested that the new cell type in the esophageal mucosa probably contains 5-hydroxytryptamine (serotonin). The serotonin-containing cells were argentaffin, but negative for Grimelius' silver stain.

An Immunohistochemical Study of Endocrine Cells in the Alimentary Tract of the Red-bellied Frog, Bombina orientalis.

“…The regional distributions and relative frequencies of the endocrine cells in the alimentary tract of the red-bellied frog resembled to those of the other anuran species [4,7,8,[15][16][17][18][19][20][21]27] except for esophagus. Although the appearance of serotonin-immunoreactive cells in the esophagus was previously reported for bullfrog [23], and even if the esophagus of the frogs was different from those of mammals, lined by pseudostratified columnar ciliated epithelium and contained numerous goblet cells scattered throughout the epithelium, the demonstration of somatostatin-immunoreactive cells in the esophagus of the anuran species were the first in this study.…”

Histochemical, immunohistochemical, and ultrastructural observations of the esophagus morphology of Rinella icterica (Anuran, Bufonidae)

“…Concerning the epithelial lining, the esophagus of R. icterica was similar to that described for L. pipiens (Norris 1959), L. catesbeianus (Nada et al, 1984), and P. perezi (Gallego-Huidobro et al 1992), having a ciliated columnar pseudostratified epithelium overlying the loose connective tissue of the mucosa. In contrast, the esophagus of P. ridibundus was lined with a nonciliated simple columnar epithelium (Geuze 1971).…”

“…Most amphibians produce more pepsinogen in the esophagus when compared with that of the stomach (Vonk 1964). The regional distribution and relative frequencies of 5-HT-immunoreactive cells were detected in the esophageal epithelium of A. regularis (El-Salhy et al 1981), L. catesbeinus (Nada et al 1984), and Bombina orientalis (Ku et al 2000). Serotonin (5-hydroxytryptamine, 5-HT) is a widespread aminergic component of secretory granules in the diffuse gastro-enteropancreatic (GEP) neuroendocrine system of mammals and lower vertebrates, where it is co-stored and co-secreted with specific corresponding hormones (Maake et al 2001), neuropeptides (D'Este et al 1994Trandaburu and Trandaburu 2007), and granin proteins (Lee and Ku 2004).…”

“…In Lithobates pipiens (formerly Rana pipiens) (Norris 1959), Amietophrynus regularis (formerly Bufo regularis) (El-Salhy et al 1981), Rhinella marina (formerly Bufo marinus) (Taylor and Tyler 1986), Pseudepidalea viridis (formerly Bufo viridis) (Liquori et al 2002), Glandirana rugosa (formerly Rana rugosa) (Ku et al 2003a), and Lithobates catesbeianus (formerly Rana catesbeiana) (Nada et al 1984), the esophagus is lined by pseudostratified columnar ciliated epithelium, which rests on a distinct basal lamina. In Lithobates pipiens (formerly Rana pipiens) (Norris 1959), Amietophrynus regularis (formerly Bufo regularis) (El-Salhy et al 1981), Rhinella marina (formerly Bufo marinus) (Taylor and Tyler 1986), Pseudepidalea viridis (formerly Bufo viridis) (Liquori et al 2002), Glandirana rugosa (formerly Rana rugosa) (Ku et al 2003a), and Lithobates catesbeianus (formerly Rana catesbeiana) (Nada et al 1984), the esophagus is lined by pseudostratified columnar ciliated epithelium, which rests on a distinct basal lamina.…”

“…Extensive studies have been carried out to analyze the endocrine cell ontogeny and distribution in several mammalian species (Eerdunchaolu et al 2001;Ku et al 2003b;Machado-Santos et al 2009). In the esophageal mucosa serotonin, somatostatin and cromogranin were detected in A. regularis (El-Salhy et al 1981), L. catesbeianus (Nada et al 1984), Pelophylax ridibundus (formerly Rana esculenta), (D'Este et al 1994), and Bombina orientalis (Ku et al 2000). In the esophageal mucosa serotonin, somatostatin and cromogranin were detected in A. regularis (El-Salhy et al 1981), L. catesbeianus (Nada et al 1984), Pelophylax ridibundus (formerly Rana esculenta), (D'Este et al 1994), and Bombina orientalis (Ku et al 2000).…”