The oestrogenic and anti-platelet activities of dihydrobenzofuroisocoumarins and homoisoflavonoids from Liriope platyphylla roots

Tsai, Yi‐Hung; Chiang, Shang Yu; El-Shazly, Mohamed; Wu, Chin Chung; Beerhues, Ludger; Lai, Wan Chun; Wu, Shou Fang; Yen, Ming Hong; Wu, Yang Chang; Chang, Fang Rong

doi:10.1016/j.foodchem.2013.02.069

Cited by 42 publications

(36 citation statements)

References 30 publications

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“…LPRP, LPRP-9, and (−)-Liriopein B were purified by us, and their purity (99.5%) was identified by HPLC and NMR analysis [11], and like positive drugs doxorubicin (Sigma-Aldrich) and resveratrol (Tocris) were dissolved in DMSO and added to the culture medium 24 or 48 hours before the cells were harvested. For each treatment, cells were exposed to different drug concentrations while solvent DMSO concentration was adjusted and limited to 0.01% (v/v).…”

Section: Methodsmentioning

confidence: 99%

Active Constituents fromLiriope platyphyllaRoot against Cancer GrowthIn Vitro

Wang

Cheng

et al. 2013

Evidence-Based Complementary and Alternative Medicine

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Liriope spicata is a well-known herb in traditional Chinese medicine, and its root has been clinically demonstrated to be effective in the treatment of metabolic and neural disorders. The constituents isolated from Liriope have also recently been shown to possess anticancer activity, although the mechanism of which remains largely unknown. Here, we illustrate the anticancer activity of LPRP-9, one of the active fractions we fractionated from the Liriope platyphylla root part (LPRP) extract. Treatment with LPRP-9 significantly inhibited proliferation of cancer cell lines MCF-7 and Huh-7 and down-regulated the phosphorylation of AKT. LPRP-9 also activates the stress-activated MPAK, JNK, p38 pathways, the p53 cell-cycle checkpoint pathway, and a series of caspase cascades while downregulating expression of antiapoptotic factors Bcl-2, Bcl-XL, and survivin. Such activities strongly suggest a role for LPRP-9 in apoptosis and autophagy. We further purified and identified the compound (−)-Liriopein B from LPRP-9, which is capable of inhibiting AKT phosphorylation at low concentration. The overall result highlights the anticancer property of LPRP-9, suggests its mechanism for inhibition of proliferation and promotion of cell death for cancer cells via regulation of multitarget pathways, and denotes the importance of purifying components of fraction LPRP-9 to aid cancer therapy.

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Section: Methodsmentioning

confidence: 99%

Active Constituents fromLiriope platyphyllaRoot against Cancer GrowthIn Vitro

Wang

Cheng

et al. 2013

Evidence-Based Complementary and Alternative Medicine

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“…Expressions of c-Fos, NFATc1, tartrate resistant-acid phosphatase (TRAP), cathepsin K, and phosphorylation of p38 induced by RANKL was inhibited by LR [27]. The ethanolic extract of LR, dihydrobenzofuroisocoumarins, and homoisoflavonoids showed potential oestrogenic and anti-platelet activities [2]. Spicatoside A and 25(s)-ruscogenin were downregulated the MMP-13 expression in IL-1-treated SW1353 cells.…”

Section: Anti-osteoclastogenesis Activitymentioning

confidence: 99%

“…Many studies have revealed steroidal saponins and their glycosides, phenolic compounds, secondary metabolites from L. platyphylla and L. spicata, whereas steroidal glycosides and homoisoflavones from O. japonicus are considered as active constituents in LR. The saponins and steroidal glycosides were tabulated in Table 1 (-)-syringaresinol, hexadecanoic acid-2',3'-dihydroxypropyl ester, indole-3-carboxylic acid, 4-hydroxybenzaldehyde, 4-hydroxybenzoicacid methyl ester, vanillin, and ethyltributanoate were isolated [2]. Moreover, methylophiopogonanone A and B [3], β-sitosterol, tran-p-hydroxycinnamic acid, 2-(4′-hydroxybenzoyl)-5,6-methylenedioxybenzofuran, 2-(4′-hydroxybenzyl)-5,6-methylenedioxy-benzofuran, 5-hydroxymethyl-2-furaldehyde, allylpyrocatechol, 2,6-dimethoxy-4-nitrophenol, syringic acid, and vanillic acid [4] were also reported from LR.…”

Section: Phytochemistrymentioning

confidence: 99%

Phytochemical and pharmacological overview on <i>Liriopes radix</i>

Mahesh¹,

Kim²

2016

Trop. J. Pharm Res

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Liriopogons (Liriope and Opiopogon) species are used as the main medicinal ingredient in traditional medicine in several Asian countries. The roots or tubers of Liriope plants (Liriopes radix; LR) have traditionally been used for hundreds of years to treat cough, insomnia, constipation, asthma, and inflammation. The present review discusses extensively the available knowledge on its phytochemical and pharmacological activities in vitro and in vivo. The review does not include other parts of these plants. Literature evidence has been analyzed to identify responsible phytochemicals and their wide range of pharmacological activities. Further studies are needed for the isolation of purified compounds in order to understand their mechanisms of action and for clinical application.

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“…Previously, the constituents from ethanolic extract of Liriope platyphylla (Liliaceae) roots have been shown to have oestrogenic, anti-platelet aggregation, and anti-cancer activities. 24 We have isolated from the extract a newly identified compound (-)-Liriopein B (LB), which is able to down-regulate Akt phosphorylation in MCF-7 breast cancer cells within a non-cytotoxic concentration range. 25 The cellular effect of LB and its potential anti-cancer mechanisms, however, are not clear.…”

Section: Inroductionmentioning

confidence: 99%

“…24 Doxorubicin and wortmannin were purchased from Sigma-Aldrich, and PP1 and AG1478 were purchased from Tocris Bioscience (Bristol, UK). All chemicals were dissolved in DMSO to make stock solutions.…”

Section: Drug Preparationsmentioning

confidence: 99%

(−)-Liriopein B Suppresses Breast Cancer Progression via Inhibition of Multiple Kinases

Wang

Chang

Huang

et al. 2015

Chem. Res. Toxicol.

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Numerous breast cancer patients who achieve an initial response to HER-targeted therapy rapidly develop resistance within one year, leading to treatment failure. Observations from clinical samples indicate that such resistance correlates with an increase in Src, EGFR, and PI3K/Akt activities and a decrease in PTEN activity. Furthermore, Akt survival signaling activation is also found in tumors treated by toxic chemotherapeutic agents. Because cotreatment with a PI3K inhibitor is a promising strategy to delay acquired resistance by preventing secondary gene activation, we therefore investigated the effects of a newly identified compound, (-)-Liriopein B (LB), on PI3K/Akt signaling activity in breast cancer cells. Our results showed that nontoxic doses of LB are able to inhibit AKT activation in both luminal-like MCF-7 and basal-like MDA-MB-231 breast cancer cells. Low doses of LB also inhibited cell migration, invasion, and cancer-stem cell sphere formation. Suppression of EGF-induced EGFR and ERK1/2 activation by LB might contribute in part to retardation of cancer progression. Furthermore, LB increases sensitivity of MDA-MB-231 cells to gefitinib in vitro, suggesting that EGFR may not be the only target of LB. Finally, a small scale in vitro kinase assay screen demonstrated that LB has a potent inhibitory effect on multiple kinases, including PI3K, Src, EGFR, Tie2, lck, lyn, RTK5, FGFR1, Abl, and Flt. In conclusion, this study demonstrates for the first time that the compound LB improves tumor therapeutic efficacy and suggests LB as a promising candidate for studying new leads in the development of kinase inhibitors.

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The oestrogenic and anti-platelet activities of dihydrobenzofuroisocoumarins and homoisoflavonoids from Liriope platyphylla roots

Cited by 42 publications

References 30 publications

Active Constituents fromLiriope platyphyllaRoot against Cancer GrowthIn Vitro

Active Constituents fromLiriope platyphyllaRoot against Cancer GrowthIn Vitro

Phytochemical and pharmacological overview on <i>Liriopes radix</i>

(−)-Liriopein B Suppresses Breast Cancer Progression via Inhibition of Multiple Kinases

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