Active Constituents fromLiriope platyphyllaRoot against Cancer GrowthIn Vitro

Abstract

Liriope spicata is a well-known herb in traditional Chinese medicine, and its root has been clinically demonstrated to be effective in the treatment of metabolic and neural disorders. The constituents isolated from Liriope have also recently been shown to possess anticancer activity, although the mechanism of which remains largely unknown. Here, we illustrate the anticancer activity of LPRP-9, one of the active fractions we fractionated from the Liriope platyphylla root part (LPRP) extract. Treatment with LPRP-9 significantly inhibited proliferation of cancer cell lines MCF-7 and Huh-7 and down-regulated the phosphorylation of AKT. LPRP-9 also activates the stress-activated MPAK, JNK, p38 pathways, the p53 cell-cycle checkpoint pathway, and a series of caspase cascades while downregulating expression of antiapoptotic factors Bcl-2, Bcl-XL, and survivin. Such activities strongly suggest a role for LPRP-9 in apoptosis and autophagy. We further purified and identified the compound (−)-Liriopein B from LPRP-9, which is capable of inhibiting AKT phosphorylation at low concentration. The overall result highlights the anticancer property of LPRP-9, suggests its mechanism for inhibition of proliferation and promotion of cell death for cancer cells via regulation of multitarget pathways, and denotes the importance of purifying components of fraction LPRP-9 to aid cancer therapy.

Clinical Experiences of Korean Medicine Treatment against Urinary Bladder Cancer in General Practice

“…Several studies on the direct effect against UBC have suggested that a homogeneous polysaccharide from Panax ginseng displayed potent antiproliferative and antimetastatic activities in human bladder T24 cells, and Rg3 ginsenoside inhibited the proliferation of EJ (human bladder transitional cell carcinoma cells) by inducing apoptosis [ 21 , 22 ]. Additionally, treatment with Liriope platyphylla significantly inhibited proliferation of MCF-7 (breast carcinoma cells) and Huh-7 (hepatic carcinoma cells) by inducing apoptosis and autophagy pathways [ 23 ]. DT-13, a saponin monomer from Liriope platyphylla , showed antiangiogenic effects mediated by reductions in vascular endothelial growth factor (VEGF), C-C chemokine receptor type 5 (CCR5), and hypoxia-inducible factor 1 α (HIF-1 α ) [ 24 ].…”

Secondary Metabolites of the Endophytic Fungus Lachnum abnorme from Ardisia cornudentata

“…After the culture supernatant had been removed for nitrite determination, we used the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) solution to assess the cell viability as previously described [27]. In brief, 0.5 mg/mL of MTT in DMEM was added to each well of cells and incubated in cell culture condition for 3 h. Then, the supernatant was removed from each well by suction, and 100 μL of DMSO was added to dissolve the formazan crystals.…”

Phytochemical and pharmacological overview on <i>Liriopes radix</i>

“…It also activates the MAPK pathways. (-)-Liriopein B is capable of inhibiting Akt phosphorylation at low concentration [66]. The nontoxic doses are able to inhibit AKT activation in both luminal-like MCF-7 and basal-like MDA-MB231 breast cancer cells.…”