The oligopeptide transport system of <i>Bacillus subtilis</i> plays a role in the initiation of sporulation

Perego, Marta; Higgins, Christopher F.; Pearce, Stephen R.; Gallagher, Maurice P.; Hoch, James A.

doi:10.1111/j.1365-2958.1991.tb01838.x

Cited by 326 publications

(287 citation statements)

References 60 publications

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“…It has turned out that Gram-positive species have the equivalent systems with binding proteins that are anchored to the membrane by a lipid group, which restricts diffusion to two dimensions (in the plane of the membrane) [76,77]. The sugar-binding protein MsmE of S. mutans has been shown to be attached to the membrane by means of an amino-terminal lipo-amino acid anchor ( Figs.…”

Section: Atp-driven Nutrient Uptake and Product/drug Excretionmentioning

confidence: 99%

Energy transduction in lactic acid bacteria

Poolman

1993

FEMS Microbiology Reviews

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In the discovery of some general principles of energy transduction, lactic acid bacteria have played an important role. In this review, the energy transducing processes of lactic acid bacteria are discussed with the emphasis on the major developments of the past 5 years. This work not only includes the biochemistry of the enzymes and the bioenergetics of the processes, but also the genetics of the genes encoding the energy transducing proteins. The progress in the area of carbohydrate transport and metabolism is presented first. Sugar translocation involving ATP-driven transport, ion-linked cotransport, heterologous exchange and group translocation are discussed. The coupling of precursor uptake to product product excretion and the linkage of antiport mechanisms to the deiminase pathways of lactic acid bacteria is dealt with in the second section. The third topic relates to metabolic energy conservation by ehemiosmotic processes. There is increasing evidence that precursor/product exchange in combination with precursor decarboxy[ation allows bacteria to generate additional metabolic energy. In the final section transport ot nutrients and ions as well as mechanisms to excrete undesirable (toxic) compounds from the cells are discussed.

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Section: Atp-driven Nutrient Uptake and Product/drug Excretionmentioning

confidence: 99%

Energy transduction in lactic acid bacteria

Poolman

1993

FEMS Microbiology Reviews

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“…Although small peptides have clear nutritional value as a source of amino acids, carbon, and nitrogen, they can have additional beneficial functions. For example, studies in bacteria have demonstrated a link between peptide transport and chemotaxis [17], sporulation [18,19], and the recycling of cell wall peptides [20].…”

Section: Introductionmentioning

confidence: 99%

Harnessing Natural Diversity to Probe Metabolic Pathways

Homann¹,

Cai²,

Becker³

et al. 2005

PLoS Genet

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Analyses of cellular processes in the yeast Saccharomyces cerevisiae rely primarily upon a small number of highly domesticated laboratory strains, leaving the extensive natural genetic diversity of the model organism largely unexplored and unexploited. We asked if this diversity could be used to enrich our understanding of basic biological processes. As a test case, we examined a simple trait: the utilization of di/tripeptides as nitrogen sources. The capacity to import small peptides is likely to be under opposing selective pressures (nutrient utilization versus toxin vulnerability) and may therefore be sculpted by diverse pathways and strategies. Hitherto, dipeptide utilization in S. cerevisiae was solely ascribed to the activity of a single protein, the Ptr2p transporter. Using high-throughput phenotyping and several genetically diverse strains, we identified previously unknown cellular activities that contribute to this trait. We find that the Dal5p allantoate/ureidosuccinate permease is also capable of facilitating di/ tripeptide transport. Moreover, even in the absence of Dal5p and Ptr2p, an additional activity-almost certainly the periplasmic asparaginase II Asp3p-facilitates the utilization of dipeptides with C-terminal asparagine residues by a different strategy. Another, as-yet-unidentified activity enables the utilization of dipeptides with C-terminal arginine residues. The relative contributions of these activities to the utilization of di/tripeptides vary among the strains analyzed, as does the vulnerability of these strains to a toxic dipeptide. Only by sampling the genetic diversity of multiple strains were we able to uncover several previously unrecognized layers of complexity in this metabolic pathway. High-throughput phenotyping facilitates the rapid exploration of the molecular basis of biological complexity, allowing for future detailed investigation of the selective pressures that drive microbial evolution.Citation: Homann OR, Cai H, Becker JM, Lindquist SL (2005) Harnessing natural diversity to probe metabolic pathways. PLoS Genet 1(6): e80.

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“…However, a few reports have shown peptide transport to be involved in bacterial sporulation (Mathiopoulos et al, 1991;Perego et al, 1991), chemotaxis in bacteria (Manson et al, 1986), and recycling of bacterial cell wall peptides (Goodell and Higgins, 1987). In plants, peptide transport has been demonstrated in isolated scutella from germinating grains of barley, wheat, rice, and maize and postulated to be involved in supplying amino acids, stored in the form of peptides, from the endosperm to the germinating embryo (Higgins and Payne, 1978a;Sopanen et al, 1978;Salmenkallio and Sopanen, 1989).…”

Section: Introductionmentioning

confidence: 99%