The optimal pressure for initial flush with UW solution in heart procurement

Mohara, Jun; Tsutsumi, Hirofumi; Takeyoshi, Izumi; Tokumine, Masahiko; Aizaki, Masahiro; Ishikawa, Susumu; Matsumoto, Koshi; Morishita, Yasuo

doi:10.1016/s1053-2498(01)00388-6

Cited by 8 publications

(7 citation statements)

References 23 publications

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“…There is no clear consensus on which solution is better than another. All have limitations on the adequacy and length of protection (43), and their protective effects are dependent on the conditions of the study (19,21,31). Many of the heart studies compare one preservation solution to another (5,8,16,20,21,24,25,30,33,35,(52)(53)(54) and with or without additives, such as channel activators or blockers (11,12,18,26,40,44,46), ion exchange inhibitors (6,42,48), anesthetics (2,40,47), or BDM (20,46,49).…”

Section: Discussionmentioning

confidence: 99%

Ten-hour preservation of guinea pig isolated hearts perfused at low flow with air-saturated Lifor solution at 26°C: comparison to ViaSpan solution

Stowe

Camara²,

Heisner³

et al. 2007

American Journal of Physiology-Heart and Circulatory Physiology

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There is no suitable solution to preserve hearts for longer than 5 h between donor explant and recipient implant. Lifor is a fully artificial preservation medium containing both a nonprotein oxygen and nutrient carrier (nanoparticles) and cellular nutrients, including amino acids and sugars. We proposed that recirculated Lifor solution would satisfactorily preserve guinea pig isolated hearts perfused at low flow with no added O(2) at room temperature for 10 h. Hearts were isolated from 21 guinea pigs and perfused with Krebs-Ringer (KR) solution (97% O(2) and 3% CO(2)) at 37 degrees C. Heart rate, inflow and outflow O(2) tension, coronary flow, left ventricular pressure (LVP), and maximal and minimal rate of change in LVP (dLVP/dt) were measured. After baseline measurements, hearts were perfused with recirculated Lifor or ViaSpan equilibrated with room air at 15% of control flow at 26 degrees C for 10 h. Hearts were then perfused at 100% flow with KR for 2 h at 37 degrees C. A time control (untreated) group was perfused only with KR solution for 15 h. Lifor arrested and protected hearts against diastolic contracture and maintained a low O(2) extraction. Compared with time controls, Lifor led to a higher developed LVP and coronary flow; %O(2) extraction and cardiac efficiency were similar between these two groups. Hearts similarly treated with ViaSpan exhibited diastolic contracture and lower %O(2) extraction during treatment and, upon reperfusion with KR, exhibited continued diastolic contracture, no return of heart rate or contractility, low coronary flow, low %O(2) extraction, and marked infarction. For long-term cardiac protection, a suitable preservation solution recirculated at low flow and room temperature without supplemental O(2) would reduce the support apparatus required for transport. Lifor was far superior to ViaSpan in meeting these requirements.

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Section: Discussionmentioning

confidence: 99%

Ten-hour preservation of guinea pig isolated hearts perfused at low flow with air-saturated Lifor solution at 26°C: comparison to ViaSpan solution

Stowe

Camara²,

Heisner³

et al. 2007

American Journal of Physiology-Heart and Circulatory Physiology

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“…Additionally, luteolin can lead to vasculature dilation (39), which may be involved in the inhibition of voltage-gated calcium channels, ligand-gated calcium channels, internal calcium release and potassium channel activation; this is consistent with the results of the current study, which demonstrate an increase in CF in the presence of luteolin. Luteolin-induced dilation may minimize endothelial cell injury caused by UW-induced vessel contraction and improve the penetration of protective components in UW, thereby improving heart protection (31,48). Thus, during long term-storage, luteolin may protect against heart injury through its effect on anti-oxidation, and by preventing calcium overload and increasing vessel dilation.…”

Section: Discussionmentioning

confidence: 99%

Luteolin improves heart preservation through inhibiting hypoxia‑dependent L‑type calcium channels in cardiomyocytes

et al. 2019

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The current study aimed to evaluate whether luteolin could improve long-term heart preservation; this was achieved by evaluating the heart following long-term storage in University of Wisconsin solution (the control group) and in solutions containing three luteolin concentrations. The effects of different preservation methods were evaluated with respect to cardiac function while hearts were in custom-made ex vivo Langendorff perfusion systems. Different preservation methods were evaluated with respect to the histology, ultrastructure and apoptosis rate of the hearts, and the function of cardiomyocytes. In the presence of luteolin, the rate pressure product of the left ventricle was increased within 60 min of reperfusion following a 12-h preservation, coronary flow was higher within 30 min of reperfusion, cardiac contractile function was higher throughout reperfusion following 12-and 18-h preservations, and the left ventricle peak systolic pressure was significantly higher compared with the control group (all P<0.05). The expression levels of apoptosis regulator Bax and apoptosis regulator Bcl-2 in the luteolin groups were significantly decreased and increased, respectively. Lactate dehydrogenase, creatine kinase and malondialdehyde enzymatic activity was increased following long-term storage, while the activity of superoxide dismutase was significantly decreased. Furthermore, luteolin inhibited L-type calcium currents in ventricular myocytes under hypoxia conditions. Thus, luteolin demonstrated protective effects during long-term heart preservation in what appeared to be a dose-dependent manner, which may be accomplished through inhibiting hypoxia-dependent L-type calcium channels.

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“…All these solutions have limitations regarding adequacy and length of protection,36 and their protective effects are dependent on study conditions 32,37,38. Many of the heart studies have compared one preservation solution with another,26,29–33,35,39–44 and with or without additives, such as channel activators or blockers,1,11,45–49 exchange inhibitors,50–52 anesthetics,2,9,49 nitric oxide (NO) donors,53 perfluorocarbons54 or BDM 1,30,55.…”

Section: Discussionmentioning

confidence: 99%

Low-flow Perfusion of Guinea Pig Isolated Hearts With 26°C Air-saturated Lifor Solution for 20 Hours Preserves Function and Metabolism

Stowe

Camara

Heisner

et al. 2008

The Journal of Heart and Lung Transplantation

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Background-Donor human hearts cannot be preserved for >5 hours between explantation and recipient implantation. A better approach is needed to preserve transplantable hearts for longer periods, ideally at ambient conditions for transport. We tested whether Lifor solution could satisfactorily preserve guinea pig isolated hearts perfused at low flow with no added oxygen at room temperature for 20 hours.

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The optimal pressure for initial flush with UW solution in heart procurement

Cited by 8 publications

References 23 publications

Ten-hour preservation of guinea pig isolated hearts perfused at low flow with air-saturated Lifor solution at 26°C: comparison to ViaSpan solution

Ten-hour preservation of guinea pig isolated hearts perfused at low flow with air-saturated Lifor solution at 26°C: comparison to ViaSpan solution

Luteolin improves heart preservation through inhibiting hypoxia‑dependent L‑type calcium channels in cardiomyocytes

Low-flow Perfusion of Guinea Pig Isolated Hearts With 26°C Air-saturated Lifor Solution for 20 Hours Preserves Function and Metabolism

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