<p>Fertilization of N, P, and K on soybean planted under <em>K</em><em>ayu Putih</em> stands to serve to increase soil nutrients. <em>Lithic Haplustert</em> is a subgroup of Vertisol with a relatively low fertility and organic matter with a heavy clay texture. This research aims to determine the response of soybean to the application of N, P, and K fertilizers on the <em>Lithic Haplustert</em> in physiology and yield. This research was conducted from February to May 2015 in the Srikoyo hamlet, Menggoran village, Playen district, Gunungkidul regency, Yogyakarta. The experimental design is split-split plot with three levels of fertilization of N (0, 25, 50 kg urea ha<sup>-1</sup>), P (0, 150, 300 kg SP-36 ha<sup>-1</sup>), and K (0, 75, 150 kg KCl ha<sup>-1</sup>). The results showed that the application of 50 kg urea ha<sup>-1 </sup>and 300 kg SP-36 ha<sup>-1</sup> can increase the leaf area and photosynthesis rate. Dosage 150 kg KCl ha<sup>-1</sup> fertilizer increased the concentration of N, P, and K in the plant tissues significantly.</p>