The optimum inhibitory effects of Alpha Interferon and Cisplatin in colon cancer, a comparative in vitro study

Abstract

Cisplatin is well known as a potent anti-cancer agent against colon cancer. However, alpha interferons are also widely used for cancer suppression. This in vitro study was designed to investigate and compare the cancer suppression function of alpha interferon in colon cancer with Cisplatin. The analysis used a human SW 480 cancer cell line with RPMI-1630 culture media. Six dilutions of interferon (2.5 μg/ml, 1.25 μg/ml, 0.562 μg/ml, 0.286 μg/ml, 0.143 μg/ml, and 0.057 μg/ml) and six dilutions of cisplatin (100 μg/ml, 50 μg/ml, 25 μg/ml, 6.25 μg/ml, and 3.125) were used at 24, 48 and 72 hours along with the presence of control groups. Following this, results were observed by ELISA plate reader, and percentage inhibition was calculated using ANOVA analysis. The interferon and cisplatin percentage of inhibition was comparable with higher inhibition rates observed with alpha interferon. The statistical analysis showed that the maximum inhibition was observed at a 0.143 μg/ml interferon concentration when exposed for 48 to 72 hours. This in vitro analysis demonstrated the anti-cancer activity of alpha interferon and its advanced inhibitory activity compared to Cisplatin.

Ropivacaine enhanced the cisplatin-sensitivity of Human colorectal cancer cells by accelerating ROS-meditated apoptosis and ferroptosis

“…Cisplatin has been shown to impair DNA repair by crosslinking with purine bases on DNA, leading to the cell death of cancer cells, it is widely used in clinical practice as the rst-line agent for the chemotherapy of colon cancer [31]. However, the drug resistance of cisplatin limited its clinical application [32].…”

Cisplatin increases carboxylesterases through increasing PXR mediated by the decrease of DEC1

“…Cisplatin or cis -diamminedichloroplatinum (CDDP) is one of the most effective and pioneering metal-based chemotherapeutic drugs [ 1 ] . It is widely used for the treatment of various solid cancers, such as cancers of head and neck, bladder, lung, cervical, colon and others [ 1 – 3 ] . Besides its strong antitumor activity and long-lasting efficacy, CDDP has some side effects, such as drug resistance and serious adverse reactions including nausea, vomiting, nephrotoxicity, hepatotoxicity, ototoxicity, and neurotoxicity [ 4 – 6 ] .…”