The order of replication of chromosomal markers in<i>Pseudomonas aeruginosa</i>strain 1: I. Marker frequency analysis by transduction

Booker, R. J.; Loutit, J. S.

doi:10.1017/s0016672300014762

Cited by 8 publications

(13 citation statements)

References 17 publications

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“…The argument was based primarily on the fact that pro-4 (the additional marker on the smaller linkage group) was replicated before the last marker of the major linkage group (ilvB). The results for pro-4 were equivocal, however, and the present investigation was begun to confirm the earlier results and to see whether the suggested organisation of Booker & Loutit (1974) Fig. 1.…”

Section: Introductionsupporting

confidence: 52%

“…The relationship between these two linkage groups is not clear and the work described in this paper and a preceding one (Booker & Loutit, 1974) was undertaken in an attempt to determine whether they are parts of a single chromosome or are separate physical structures.…”

Section: Introductionmentioning

confidence: 99%

“…In the preceding paper (Booker & Loutit, 1974) we reported the results of marker frequency analysis based on the ratios of transductants from phage lysates prepared from fast and slow-growing cells of Pseudomonas aeruginosa strain 1. The results showed that replication could be bidirectional from an origin near the trp-1 marker and that at least three of the four markers on the smaller linkage group reported by Loutit (1969) were replicated later than the genes on the major linkage group.…”

Section: Introductionmentioning

confidence: 99%

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The replication of the chromosome of Pseudomonas aeruginosa strain 1: II. Sequential mutagenesis of synchronized cultures

Booker

Loutit

1975

Genet. Res.

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SUMMARYThe order of replication of a series of genes in Pseudomonas aeruginosa has been studied in synchronized cultures using a method based on the technique of sequential mutagenesis. This technique relies on the increased susceptibility of the replication point of the bacterial chromosome to mutagenesis by N-methyl-N′-nitro-N-nitrosoguanidine. The genes studied were those previously mapped by conjugation and whose order of replication had been studied by an investigation of gene frequencies in exponential populations. The results are consistent with the idea that there is two-way replication of the chromosome of P. aeruginosa starting at a point near trp-1 and arg-6. They also confirm that the two linkage groups which have been found by conjugation replicate at different times. If the assumption is made that there is only one chromosome in P. aeruginosa, the results can be used to show how the two linkage groups may possibly be joined together and the order is such that there would have to be two sites of attachment for the sex factor FP2.

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Section: Introductionsupporting

confidence: 52%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The replication of the chromosome of Pseudomonas aeruginosa strain 1: II. Sequential mutagenesis of synchronized cultures

Booker

Loutit

1975

Genet. Res.

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“…There have been reports of natural transformation of chromosomal DNA in P. fluorescens (19,20) and in P. aeruginosa (17,18), but these observations have not been confirmable (5,22,25). Although the transformability of P. solanacearum has been cited (11) it is not clear that the observed phenomenon was not, in fact, conjugation (6).…”

Section: Discussionmentioning

confidence: 99%

Pseudomonas stutzeri and related species undergo natural transformation

Carlson¹,

Pierson²,

Rosen³

et al. 1983

J Bacteriol

153

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Cells of Pseudomonas stutzeri are naturally transformed by homologous chromosomal DNA; they do not require chemical treatment to become competent. This capacity to undergo natural transformation was found to be shared by the closely related species P. mendocina, P. alcaligenes, and P. pseudoalcaligenes, but was not detectable in strains of P. aeruginosa, P. perfectomarinus, P. putida, P. fluorescens, or P. syringae. P. stutzeri could be transformed either on plates or in liquid medium. Only double-stranded chromosomal DNA was effective; single-stranded DNA and plasmid DNA were not. DNA fragments larger than 10 kilobase pairs were more effective than smaller fragments. The

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“…In the 1970s, Booker and Loutit proposed that Pseudomonas aeruginosa strain PAO1 has two chromosomes, based on conjugational linkage studies (Booker & Loutit, 1974). This later proved incorrect.…”

Section: Introductionmentioning

confidence: 99%

Maintenance of chromosome structure inPseudomonas aeruginosa

Rybenkov

2014

FEMS Microbiol Lett

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Replication and segregation of genetic information is an activity central to the well-being of all living cells. Concerted mechanisms have evolved that ensure that each cellular chromosome is replicated once and only once per cell cycle and then faithfully segregated into daughter cells. Despite remarkable taxonomic diversity, these mechanisms are largely conserved across eubacteria, although species specific distinctions can often be noted. Here, we provide an overview of the current state of knowledge about maintenance of the chromosome structure in Pseudomonas aeruginosa. We focus on global chromosome organization and its dynamics during DNA replication and cell division. Special emphasis is made on contrasting these activities in P. aeruginosa and other bacteria. Among unique P. aeruginosa features are the presence of two distinct autonomously replicating sequences and multiple condensins, which suggests existence of novel regulatory mechanisms.

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The order of replication of chromosomal markers inPseudomonas aeruginosastrain 1: I. Marker frequency analysis by transduction

Cited by 8 publications

References 17 publications

The replication of the chromosome of Pseudomonas aeruginosa strain 1: II. Sequential mutagenesis of synchronized cultures

The replication of the chromosome of Pseudomonas aeruginosa strain 1: II. Sequential mutagenesis of synchronized cultures

Pseudomonas stutzeri and related species undergo natural transformation

Maintenance of chromosome structure inPseudomonas aeruginosa

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