The Organism Is the Product

Kelly, Jason

doi:10.1021/sb2000287

Cited by 2 publications

(2 citation statements)

References 2 publications

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“…Since the advances of synthetic biology are enabling the construction of increasingly complex controllable systems [12] and industrially relevant solutions [27], predictability has become a crucial point for the realization and debugging of customized technologies. With this work, we intend to provide useful data to support the predictable tuning of gene expression in recombinant E. coli cultures.…”

Section: Introductionmentioning

confidence: 99%

Half-life measurements of chemical inducers for recombinant gene expression

et al. 2014

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BackgroundInducible promoters are widely spread genetic tools for triggering, tuning and optimizing the expression of recombinant genes in engineered biological systems. Most of them are controlled by the addition of a specific exogenous chemical inducer that indirectly regulates the promoter transcription rate in a concentration-dependent fashion. In order to have a robust and predictable degree of control on promoter activity, the degradation rate of such chemicals should be considered in many applications like recombinant protein production.ResultsIn this work, we use whole-cell biosensors to assess the half-life of three commonly used chemical inducers for recombinant Escherichia coli: Isopropyl β-D-1-thiogalactopyranoside (IPTG), anhydrotetracycline (ATc) and N-(3-oxohexanoyl)-L-homoserine lactone (HSL). A factorial study was conducted to investigate the conditions that significantly contribute to the decay rate of these inducers. Temperature has been found to be the major factor affecting ATc, while medium and pH have been found to highly affect HSL. Finally, no significant degradation was observed for IPTG among the tested conditions.ConclusionsWe have quantified the decay rate of IPTG, ATc and HSL in many conditions, some of which were not previously tested in the literature, and the main effects affecting their degradation were identified via a statistics-based framework. Whole-cell biosensors were successfully used to conduct this study, yielding reproducible measurements via simple multiwell-compatible assays. The knowledge of inducer degradation rate in several contexts has to be considered in the rational design of synthetic biological systems for improving the predictability of induction effects, especially for prolonged experiments.

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Section: Introductionmentioning

confidence: 99%

Half-life measurements of chemical inducers for recombinant gene expression

et al. 2014

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“…The 'Standard European Vector Architecture' defines rules for plasmids with respect to restriction sites, overall plasmid architecture, and nomenclature that immensely facilitate the exchange of engineering back to the University of California at Berkeley and isoprene metabolism engineering in the laboratory of Jay Keasling [52] ) and Synthetic Genomics (going back to the earlier genome synthesis efforts of efforts around Craig Venter [9] ) had a bit of a mixed success, more recent endeavors such as Ginkgo Bioworks seem to be very successful in acquiring funding and attracting projects. [53] Complementing (and fueling) these efforts is a commercial DNA synthesis ecosystem in which competition and new technologies constantly reduce the cost of large scale DNA synthesis. [54] So in general, it is probably fair to say that Synthetic Biology has indeed been adopted into the world of commercial enterprise.…”

Section: The Chassis Problemmentioning

confidence: 99%

Taming the Beast of Biology: Synthetic Biology and Biological Systems Engineering

Panke

2020

Chimia

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Despite the availability of a variety of ' -omics ' technologies to support the system-wide analysis of industrially relevant microorganisms, the manipulation of strains towards an economically relevant goal remains a challenge. Remarkably, our ability to catalogue the participants in and model ever more comprehensive aspects of a microorganism's physiology is now complemented by technologies that permanently expand the scope of engineering interventions that can be imagined. In fact, genome-wide editing and re-synthesis of microbial and even eukaryotic chromosomes have become widely applied methods. At the heart of this emerging system-wide engineering approach, often labelled ' Synthetic Biology ' , is the continuous improvement of large-scale DNA synthesis, which is put to two-fold use: (i) starting ever more ambitious efforts to re-write existing and coding novel molecular systems, and (ii) designing and constructing increasingly sophisticated library technologies, which has led to a renaissance of directed evolution in strain engineering. Here, we briefly review some of the critical concepts and technological stepping-stones of Synthetic Biology on its way to becoming a mature industrial technology.

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The Organism Is the Product

Cited by 2 publications

References 2 publications

Half-life measurements of chemical inducers for recombinant gene expression

Half-life measurements of chemical inducers for recombinant gene expression

Taming the Beast of Biology: Synthetic Biology and Biological Systems Engineering

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