The ornamental fighting fish is the next model organism for genetic studies

Yue, Gen Hua; Wang, Le; Sun, Fei; Yang, Zituo; Shen, Yubang; Meng, Zining; Alfiko, Yuzer

doi:10.1111/raq.12681

Cited by 10 publications

(13 citation statements)

References 102 publications

(335 reference statements)

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“…Body coloration, including skin and fin coloration, varies among fish 114,115 . Coloration evolved through selection and adaptation 116 .…”

Section: Status Of Breeding Salinity‐tolerant Tilapiamentioning

confidence: 99%

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Status of conventional and molecular breeding of salinity‐tolerant tilapia

Yue

Xia

2023

Reviews in Aquaculture

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Aquaculture is an important sector for ensuring global food security. Due to the scarcity of freshwater available for expanding aquaculture, the development of omnivorous fish species and varieties that can tolerate high salinity will enhance fish production. Some tilapia species are good candidates for aquaculture in brackish and seawater because they can grow in high salinity. Among tilapia species, Oreochromis mossambicus, Oreochromis aureus, Oreochromis spilurus, Oreochromis urolepis hornorum, Sarotherodon galilaeus and Coptodon zillii are the most salinity‐tolerant. Hybrids derived from salinity‐tolerant tilapia species are tolerant to a certain level of salinity. They have been used in aquaculture production in brackish water and full seawater. Conventional selective breeding has been applied to increase the growth rate of salinity‐tolerant tilapias. However, their growth rate is lower than that of the freshwater Oreochromis niloticus. Recently, many genomic resources and tools have been developed for salinity‐tolerant tilapia. Quantitative trait locus (QTL) mapping and genome‐wide association studies (GWAS) for important economic traits, including salinity tolerance and other desired traits, have been carried out and applied in molecular breeding for superior salinity‐tolerant tilapia lines. In this review, we systematically analysed tilapia species that can be cultured in brackish and saltwater. We summarized previous works in conventional breeding and molecular breeding for salinity‐tolerant tilapia. We pointed out a few known and potential challenges in the selective breeding and culture of salinity‐tolerant tilapia. Due to the rapid advances in molecular and other disruptive technologies, we are optimistic that novel breeding approaches will significantly increase the production salinity‐tolerant tilapia.

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“…Body coloration, including skin and fin coloration, varies among fish 114,115 . Coloration evolved through selection and adaptation 116 .…”

Section: Status Of Breeding Salinity‐tolerant Tilapiamentioning

confidence: 99%

“…Coloration evolved through selection and adaptation 116 . In aquaculture, fish colour is associated with commercial value 114,115 . In O. mossambicus , the inheritance of gold and dark skin colours in nine backcross pedigrees showed that the gold phenotype was recessive and determined by a single locus.…”

Section: Status Of Breeding Salinity‐tolerant Tilapiamentioning

confidence: 99%

Status of conventional and molecular breeding of salinity‐tolerant tilapia

Yue

Xia

2023

Reviews in Aquaculture

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“…Due to their morphological and phenotypic diversity, relatively compact genome size (~440 Mb), their ease of growth in the laboratory, and their amenability to behavioral and neurobiological experimentation, betta have become an increasingly popular organism for scientific study (Lichak et al, 2022). The recent publication of high-quality reference genomes of both domesticated and wild betta has allowed for in-depth genetic and genomic analyses of sex determination, phenotypic traits such as pigmentation, fin shape and aggression, and of the evolutionary relationships between Betta species (Fan et al, 2018;Prost et al, 2020;Wang et al, 2021Wang et al, , 2022Kwon et al, 2022;Yue et al, 2022;Zhang et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

“…Nevertheless, the asynchronous egg fertilization resulting from a protracted mating process that can last many hours, coupled with a short interval between fertilization and cell division, as well as a thick chorion, constitute significant challenges for genetic manipulation (Valentin et al, 2015;Lichak et al, 2022). Although a few studies have successfully utilized these tools, their reported success rates have been low, and none have reported germline transmission (Wang et al, 2021(Wang et al, , 2022Yue et al, 2022). Furthermore, although plasmid DNA microinjection has been used to make transgenic commercial betta (GloFish® Betta), the more controlled and efficient Tol2-based approach has not been performed.…”

Section: Introductionmentioning

confidence: 99%

Genetic manipulation of betta fish

Palmiotti

Lichak

Shih

et al. 2023

Preprint

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Betta splendens, also known as Siamese fighting fish or 'betta', are renowned for their astonishing morphological diversity and extreme aggressive behavior. Despite recent advances in our understanding of the genetics and neurobiology of betta, the lack of tools to manipulate their genome has hindered progress at functional and mechanistic levels. In this study, we outline the use of three genetic manipulation technologies, which we have optimized for use in betta: CRISPR/Cas9-mediated knockout, CRISPR/Cas9-mediated knockin, and Tol2-mediated transgenesis. We knocked out three genes:alkal2l,bco1l, andmitfa, and analyzed their effects on viability and pigmentation. Furthermore, we successfully knocked in a fluorescent protein into themitfalocus, a proof-of-principle experiment of this powerful technology in betta. Finally, we used Tol2-mediated transgenesis to create fish with ubiquitous expression of GFP, and then developed a bicistronic plasmid with heart-specific expression of a red fluorescent protein to serve as a visible marker of successful transgenesis. Our work highlights the potential for the genetic manipulation of betta, providing valuable resources for the effective use of genetic tools in this animal model.

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“…In the past 30 years, many genomic resources, such as mtDNA (mitochondrial DNA) markers, microsatellites, SNPs (single nucleotide polymorphisms), as well as genotyping platforms, SNP arrays, linkage maps, bacterial artificial chromosome (BAC) libraries, physical maps, transcriptomes and draft reference genome sequences, have been developed for some aquaculture species 1–8 . These genomic resources have been applied in investigating genetic diversity, population structures, molecular parentage, mapping quantitative trait loci (QTL) for economically important traits, genome‐wide association study (GWAS) for traits, MAS, GS and GE in some aquaculture species 2,9,10 …”

Section: Introductionmentioning

confidence: 99%

Genomic resources and their applications in aquaculture of Asian seabass (Lates calcarifer)

Yue

Wang

Yang

et al. 2022

Reviews in Aquaculture

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Genomic resources, including nuclear DNA markers, mitochondrial DNA markers, highresolution linkage maps, transcriptomes, reference genome sequences and their annotations, are critically important in aquaculture. Their potential applications are plenty, such as enabling the analysis of genetic diversity in population structures, mating and spawning behaviour; genetic traceability; reconstructing pedigrees; mapping quantitative trait loci (QTL) for important phenotypic traits; marker-assisted selection and genomic selection; and to understand genetic and epigenetic regulation of traits. Asian seabass (Lates calcarifer) is becoming a popular aquaculture species. In the past 30 years, many genomic resources have been developed and used to understand the genetic diversity of wild and cultured populations, setting up mating schemes, genetic traceability, mapping QTL and genome-wide association studies for growth, omega-3 fatty acid content, resistance to diseases and genetic improvement of important traits in breeding programmes. In this review, we systematically synthesize the status of available genomic resources and their applications in enhancing the genetic improvement and aquaculture production of Asian seabass. We also critically discuss the challenges and future research directions of developing, optimizing and applying genomic resources in Asian seabass aquaculture.

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The ornamental fighting fish is the next model organism for genetic studies

Cited by 10 publications

References 102 publications

Status of conventional and molecular breeding of salinity‐tolerant tilapia

Status of conventional and molecular breeding of salinity‐tolerant tilapia

Genetic manipulation of betta fish

Genomic resources and their applications in aquaculture of Asian seabass (Lates calcarifer)

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