The Osmotic Hypersensitivity of the YeastSaccharomyces cerevisiae is Strain and Growth Media Dependent: Quantitative Aspects of the Phenomenon

Blomberg, Anders

doi:10.1002/(sici)1097-0061(199705)13:6<529::aid-yea103>3.0.co;2-h

Cited by 28 publications

(7 citation statements)

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“…At 45 °C under high (9X) dextrose concentration, cells were found to lose their culturability completely. Earlier research found that the activity of β-fructofuranosidase ( SUC2 ) of S. cerevisiae , which is liable for sucrose degradation; might be repressed by the increased osmotic pressure [28, 32]. This is to be mentioned that when the cells were grown at 32.5 °C in different sucrose concentrations, all were found to grow after a certain incubation period (results not shown).…”

Section: Results Interpretation and Analysismentioning

confidence: 99%

“…Abrupt changes in the environmental and physicochemical stimuli including temperature, pH, sugar/salt concentrations, the redox state, toxic compounds and nutrient exhaustion have been mostly found to elicit a battery of defending response by up-regulating the genes encoding heat shock proteins (HSPs) in bacterial cells [19–27]. Like bacteria, the heat shock response in Saccharomyes cerevisiae , the model experimental yeast species, has been also characterized by the rapid changes in their cellular physiology including the budding manner accompanied with the increased tolerance against elevated salt and sugar concentrations, and against reactive oxygen species (ROS) [1–4, 7, 9–11, 15, 18, 28–30]. In S. cerevisiae , heat-sensitivity is ordinarily prescriptive of defects in protein coding genes which are also essential for maintaining the cell viability [10, 19, 24, 31–34].…”

Section: Resultsmentioning

confidence: 99%

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Influence of heat shock and osmotic stresses on the growth and viability of Saccharomyces cerevisiae SUBSC01

2015

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BackgroundWith a preceding scrutiny of bacterial cellular responses against heat shock and oxidative stresses, current research further investigated such impact on yeast cell. Present study attempted to observe the influence of high temperature (44–46 °C) on the growth and budding pattern of Saccharomyces cerevisiae SUBSC01. Effect of elevated sugar concentrations as another stress stimulant was also observed. Cell growth was measured through the estimation of the optical density at 600 nm (OD600) and by the enumeration of colony forming units on the agar plates up to 450 min.ResultsSubsequent transformation in the yeast morphology and the cellular arrangement were noticed. A delayed and lengthy lag phase was observed when yeast strain was grown at 30, 37, and 40 °C, while at 32.5 °C, optimal growth pattern was noticed. Cells were found to lose culturability completely at 46 °C whereby cells without the cytoplasmic contents were also observed under the light microscope. Thus the critical growth temperature was recorded as 45 °C which was the highest temperature at which S. cerevisiae SUBSC01 could grow. However, a complete growth retardation was observed at 45 °C with the high concentrations of dextrose (0.36 g/l) and sucrose (0.18 g/l). Notably, yeast budding was found at 44 and 45 °C up to 270 min of incubation, which was further noticed to be suppressed at 46 °C.ConclusionsPresent study revealed that the optimal and the critical growth temperatures of S. cerevisiae SUBSC01 were 32.5 and 45 °C, respectively; and also projected on the inhibitory concentrations of sugars on yeast growth at that temperature.

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Section: Results Interpretation and Analysismentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Influence of heat shock and osmotic stresses on the growth and viability of Saccharomyces cerevisiae SUBSC01

2015

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“…In addition to possible contributions of the ald6Δ mutation and/or eutE expression to osmotolerance, the lag phases of gpd1Δ gpd2Δ strains in high-osmolarity cultures may have obscured this interesting phenotype in previous short-term growth studies [20, 21]. Osmotolerance in S. cerevisiae is a complex, multi-gene phenotype [71] and, especially upon sudden exposure to osmotic stress, G3PDH-independent mechanisms have been proposed to contribute to osmotolerance [64, 72], such as trehalose accumulation in osmotically challenged cultures growing on galactose [73]. Alternatively, intracellular glycerol could be derived via a G3PDH-independent pathway by de-acylation of acyl-glycerol-3-phosphate, which can be formed from dihydroxyacetone phosphate (DHAP) by the combined activities of DHAP acyltransferase and NADPH-linked 1-acylglycerol-3-phosphate acyltransferase [74].…”

Section: Discussionmentioning

confidence: 99%

Metabolic engineering strategies for optimizing acetate reduction, ethanol yield and osmotolerance in Saccharomyces cerevisiae

Papapetridis

Dijk

Maris

et al. 2017

Biotechnol Biofuels

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BackgroundGlycerol, whose formation contributes to cellular redox balancing and osmoregulation in Saccharomyces cerevisiae, is an important by-product of yeast-based bioethanol production. Replacing the glycerol pathway by an engineered pathway for NAD+-dependent acetate reduction has been shown to improve ethanol yields and contribute to detoxification of acetate-containing media. However, the osmosensitivity of glycerol non-producing strains limits their applicability in high-osmolarity industrial processes. This study explores engineering strategies for minimizing glycerol production by acetate-reducing strains, while retaining osmotolerance.Results GPD2 encodes one of two S. cerevisiae isoenzymes of NAD+-dependent glycerol-3-phosphate dehydrogenase (G3PDH). Its deletion in an acetate-reducing strain yielded a fourfold lower glycerol production in anaerobic, low-osmolarity cultures but hardly affected glycerol production at high osmolarity. Replacement of both native G3PDHs by an archaeal NADP+-preferring enzyme, combined with deletion of ALD6, yielded an acetate-reducing strain the phenotype of which resembled that of a glycerol-negative gpd1Δ gpd2Δ strain in low-osmolarity cultures. This strain grew anaerobically at high osmolarity (1 mol L−1 glucose), while consuming acetate and producing virtually no extracellular glycerol. Its ethanol yield in high-osmolarity cultures was 13% higher than that of an acetate-reducing strain expressing the native glycerol pathway.ConclusionsDeletion of GPD2 provides an attractive strategy for improving product yields of acetate-reducing S. cerevisiae strains in low, but not in high-osmolarity media. Replacement of the native yeast G3PDHs by a heterologous NADP+-preferring enzyme, combined with deletion of ALD6, virtually eliminated glycerol production in high-osmolarity cultures while enabling efficient reduction of acetate to ethanol. After further optimization of growth kinetics, this strategy for uncoupling the roles of glycerol formation in redox homeostasis and osmotolerance can be applicable for improving performance of industrial strains in high-gravity acetate-containing processes.Electronic supplementary materialThe online version of this article (doi:10.1186/s13068-017-0791-3) contains supplementary material, which is available to authorized users.

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“…NaCl was added at 300 mM, 700 mM, and 1.0 M to the YPD solid medium (1% yeast extract, 2% peptone, 2% d ‐glucose) at 30 °C. These salt concentrations were much higher than those used for the rice experiments because yeast is highly salt tolerant . For control images, strains were also grown in the absence of exogenous NaCl.…”

Section: Methodsmentioning

confidence: 99%

Salt‐Treated Roots of Oryza australiensis Seedlings are Enriched with Proteins Involved in Energetics and Transport

et al. 2019

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Salinity is a major constraint on rice productivity worldwide. However, mechanisms of salt tolerance in wild rice relatives are unknown. Root microsomal proteins are extracted from two Oryza australiensis accessions contrasting in salt tolerance. Whole roots of 2‐week‐old seedlings are treated with 80 mM NaCl for 30 days to induce salt stress. Proteins are quantified by tandem mass tags (TMT) and triple‐stage Mass Spectrometry. More than 200 differentially expressed proteins between the salt‐treated and control samples in the two accessions (p‐value <0.05) are found. Gene Ontology (GO) analysis shows that proteins categorized as “metabolic process,” “transport,” and “transmembrane transporter” are highly responsive to salt treatment. In particular, mitochondrial ATPases and SNARE proteins are more abundant in roots of the salt‐tolerant accession and responded strongly when roots are exposed to salinity. mRNA quantification validated the elevated protein abundances of a monosaccharide transporter and an antiporter observed in the salt‐tolerant genotype. The importance of the upregulated monosaccharide transporter and a VAMP‐like protein by measuring salinity responses of two yeast knockout mutants for genes homologous to those encoding these proteins in rice are confirmed. Potential new mechanisms of salt tolerance in rice, with implications for breeding of elite cultivars are also discussed.

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The Osmotic Hypersensitivity of the YeastSaccharomyces cerevisiae is Strain and Growth Media Dependent: Quantitative Aspects of the Phenomenon

Cited by 28 publications

References 28 publications

Influence of heat shock and osmotic stresses on the growth and viability of Saccharomyces cerevisiae SUBSC01

Influence of heat shock and osmotic stresses on the growth and viability of Saccharomyces cerevisiae SUBSC01

Metabolic engineering strategies for optimizing acetate reduction, ethanol yield and osmotolerance in Saccharomyces cerevisiae

Salt‐Treated Roots of Oryza australiensis Seedlings are Enriched with Proteins Involved in Energetics and Transport

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