The osteogenic differentiation of rat muscle-derived stem cells in vivo within in situ-forming chitosan scaffolds

Kim, Kyung Sook; Lee, Jung Hwa; Ahn, Hyun Hee; Lee, Ju Young; Khang, Gilson; Lee, Bong; Lee, Hai Bang; Kim, Moon Suk

doi:10.1016/j.biomaterials.2008.08.005

Cited by 61 publications

(56 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Recent studies have shown that the addition of glycerol phosphate disodium salt (GP) to CTS makes it possible to obtain a liquid solution at room temperature (RT). By means of the body temperature, this injectable solution undergoes a phase transition and becomes a gel (28,29). This behavior was observed to be related to electrostatic interactions between positively charged amine groups of CTS and phosphate anions of GP (29).…”

Section: Originalmentioning

confidence: 97%

See 1 more Smart Citation

<i>In vivo</i> evaluation of chitosan-glycerol gel scaffolds seeded with stem cells for full-thickness mandibular bone regeneration

et al. 2017

View full text Add to dashboard Cite

The aim of this study was to evaluate in vivo bone regeneration, mediated by adipose-derived stem cells (ADSCs), induced to differentiate into osteoblasts and carried by a scaffold gel. In the test group, bone regeneration was mediated by ADSCs, induced to differentiate into osteoblasts, and carried by a scaffold gel. In the control group a scaffold without cells was used. The scaffold, consisting of chitosan and glycerol phosphate, was maintained in situ by a cross-linked resorbable membrane. The osteogenic potential of ADSCs was confirmed by osteocalcin assay and Von Kossa staining performed before implantation. Histological assays detected an initial increase in bone formation in the test group compared with the control group. Microcomputed tomography analysis did not show significant differences between the two groups. Both histological and microcomputed tomography analysis were performed on the ex vivo specimens after a follow-up period of 8 weeks. We observed that differentiated ADSCs could increase bone regeneration and that the scaffold used here can be a suitable carrier to entrap and maintain the cells in situ. On the contrary, the membrane used was not functional in isolating the site of the defect from surrounding soft tissues and caused a significant inflammatory reaction.

show abstract

Section: Originalmentioning

confidence: 97%

“…Chitosan scaffolds preparation CTS scaffolds were prepared as described elsewhere (28,29). In brief, a CTS solution was obtained by dissolving the polymer in 0.1 M acetic acid solution prepared in deionized water (DW) at a final concentration of 2.2% w/v (polymer/solvent) and sterilized by autoclave.…”

Section: Osteogenic Differentiationmentioning

confidence: 99%

<i>In vivo</i> evaluation of chitosan-glycerol gel scaffolds seeded with stem cells for full-thickness mandibular bone regeneration

et al. 2017

View full text Add to dashboard Cite

show abstract

“…Similarly, Usas et al (2009) have investigated the delivery of BMP4-secreting muscle-derived stem cells (MDSC-B4) capable of inducing bone formation in mice by using collagen gel (CG), fibrin sealant (FS), and gelatin sponge carriers. When compared with spongeous material, gel scaffolds may represent an even more suitable treatment option for bone tissue engineering applications (Kim et al 2008). One highly important aspect that makes gel scaffolds so attractive is the possibility of injecting these cell-seeded gels into a defect area rather than following the route of implanting them through open wound surgery.…”

Section: Osteogenesismentioning

confidence: 99%

Muscle-derived stem cells: isolation, characterization, differentiation, and application in cell and gene therapy

Wang

Chen

et al. 2010

Cell Tissue Res

View full text Add to dashboard Cite

Muscle tissue represents an abundant, accessible, and replenishable source of adult stem cells for cell-based tissue and genetic engineering. A population of cells isolated from muscle exhibits both multipotentiality and self-renewal capabilities. Satellite cells, referred to by many investigators as muscle stem cells, are myogenic precursors that are capable of regenerating muscle and that demonstrate self-renewal properties; however, they are considered to be committed to the myogenic lineage. Muscle-derived stem cells (MDSCs), which may represent a predecessor of the satellite cell, are considered to possess a higher regeneration capacity and to exhibit better cell survival and a broader range of multilineage capabilities. Remarkably, MDSCs are not only able to differentiate into mesodermal cell types including the myogenic, adipogenic, osteogenic, chondrogenic, endothelial, and hematopoietic lineages, but also possess the potential to break germ layer commitment and differentiate into ectodermal lineages including neuron-like cells under certain conditions. This article reviews the current preclinical studies and potential clinical applications of MDSC-mediated gene therapy and tissue-engineering and methods for MDSC isolation, differentiation, and molecular characterization.

show abstract

“…Electrostatic interaction can occur between the polycationic chitosan and anionic compounds such as anionic glycerol phosphate disodium salt, acyllic acid, sodium deoxycholate, polylactide, hyaluronic acid, thioglycolic acid, calcium phosphate cement, fucoidan or heparin. The chitosan solutions with and without anionic compounds has shown temperature-dependent viscosity changes to give in-situ-forming chitosan scaffolds with the desired mechanical properties [7].…”

Section: Injectable In-situ-forming Scaffolds Formed By Electrostaticmentioning

confidence: 99%

Injectable In‐Situ ‐Forming Scaffolds for Tissue Engineering

Kim

Min

et al. 2012

Biomimetic Approaches for Biomaterials Development

Self Cite

View full text Add to dashboard Cite

The osteogenic differentiation of rat muscle-derived stem cells in vivo within in situ-forming chitosan scaffolds

Cited by 61 publications

References 25 publications

<i>In vivo</i> evaluation of chitosan-glycerol gel scaffolds seeded with stem cells for full-thickness mandibular bone regeneration

<i>In vivo</i> evaluation of chitosan-glycerol gel scaffolds seeded with stem cells for full-thickness mandibular bone regeneration

Muscle-derived stem cells: isolation, characterization, differentiation, and application in cell and gene therapy

Injectable In‐Situ ‐Forming Scaffolds for Tissue Engineering

Contact Info

Product

Resources

About