The Other Helicobacters

Smet, Annemieke; Flahou, Bram; Mukhopadhya, Indrani; Ducatelle, Richard; Pasmans, Frank; Haesebrouck, Freddy; Hold, Georgina L.

doi:10.1111/j.1523-5378.2011.00884.x

Cited by 13 publications

(13 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The most studied GH species so far is Helicobacter pylori , being considered the main cause of gastritis and peptic ulcer in humans and has been determined to be an important risk factor in the development of gastric adenocarcinoma and lymphoma of lymphoid tissue associated with mucosa (MALT) (Baele et al, ). However, during the last few years EHH species have become increasingly important as emerging pathogens and potential zoonotic agents (Collado et al, ), demonstrating their association with several human diseases like acute gastroenteritis, inflammatory bowel disease and hepatobiliary diseases (Solnick and Schauer, ; Smet et al, ; Rossi and Hänninnen, ). Unfortunately, there is no specific isolation protocol for these species, but occasionally, they are isolated using culture medium and atmospheric conditions designed for the taxonomic close related genus Campylobacter .…”

Section: Introductionmentioning

confidence: 99%

Comparison of media and growth conditions for culturing enterohepaticHelicobacterspecies

Ochoa

Martínez

Fernández

et al. 2019

Lett Appl Microbiol

View full text Add to dashboard Cite

This research aims to compare the culturing conditions for enterohepatic Helicobacter, evaluating culture media, incubation atmosphere and susceptibility to antimicrobials used to generate selective conditions. Four common media for the closely related genus Campylobacter (Columbia, Bolton, Brucella and CCDA agar), as well as the need for hydrogen in the microaerobic incubation atmosphere, were evaluated. Serial dilutions of 13 strains belonging to six species (H. apodemus, H. bilis, H. canicola, H. canis, H. equorum and Helicobacter sp.) were inoculated in each media and incubated at 37°C for 48 to 96 h using CampyGen (OXOID) and gaseous exchange (including hydrogen) in parallel. Columbia or Brucella agars were the most appropriate for culturing EHH (P < 0·05). However, there was no significant difference between the atmospheres evaluated (P = 0·13). In addition, minimal inhibitory concentration for six antibiotics showed that all isolates were resistant to trimethoprim, whereas for the rest of the antibiotics (cephalothin, cefoperazone, cefsulodin, teicoplanin and vancomycin) the inhibition range was between 8 and 64 μg ml−1. Our findings suggest that Columbia or Brucella media, regardless of the use of hydrogen, can be used for the EHH isolation. In addition, the concentration of antibiotics included in commercial campylobacteria supplements is suitable for EHH species recovery. Significance and Impact of the Study Enterohepatic Helicobacter (EHH) infections have been associated with several diseases in humans such as acute gastroenteritis, inflammatory bowel disease and hepatobiliary diseases. Although they are frequently detected in clinical samples by molecular methods, only occasionally they are isolated using culture conditions described for the taxonomic related pathogen Campylobacter sp. This is because the optimal conditions for the isolation of EHH have not yet been described, which results in an underestimation of the prevalence and clinical importance of these emerging pathogens. Therefore, this study provides insight for culturing EHH species.

show abstract

Section: Introductionmentioning

confidence: 99%

Comparison of media and growth conditions for culturing enterohepaticHelicobacterspecies

Ochoa

Martínez

Fernández

et al. 2019

Lett Appl Microbiol

View full text Add to dashboard Cite

show abstract

“…Among them, H. pylori is the major pathogenic bacterium in human beings. Besides H. pylori , some NPHS were also found to associate with human body function disorders [8]. For instance, H. heilmannii, H. winghamensis, H. pullorum , and H. canis were considered as causative agent of stomach and intestinal diseases [9–11].…”

Section: Introductionmentioning

confidence: 99%

Comparative Genomics ofH. pyloriand Non-PyloriHelicobacterSpecies to Identify New Regions Associated with Its Pathogenicity and Adaptability

Cao

et al. 2016

BioMed Research International

View full text Add to dashboard Cite

The genus Helicobacter is a group of Gram-negative, helical-shaped pathogens consisting of at least 36 bacterial species. Helicobacter pylori (H. pylori), infecting more than 50% of the human population, is considered as the major cause of gastritis, peptic ulcer, and gastric cancer. However, the genetic underpinnings of H. pylori that are responsible for its large scale epidemic and gastrointestinal environment adaption within human beings remain unclear. Core-pan genome analysis was performed among 75 representative H. pylori and 24 non-pylori Helicobacter genomes. There were 1173 conserved protein families of H. pylori and 673 of all 99 Helicobacter genus strains. We found 79 genome unique regions, a total of 202,359bp, shared by at least 80% of the H. pylori but lacked in non-pylori Helicobacter species. The operons, genes, and sRNAs within the H. pylori unique regions were considered as potential ones associated with its pathogenicity and adaptability, and the relativity among them has been partially confirmed by functional annotation analysis. However, functions of at least 54 genes and 10 sRNAs were still unclear. Our analysis of protein-protein interaction showed that 30 genes within them may have the cooperation relationship.

show abstract

“…Other NHPHs are Helicobacter felis , Helicobacter bizzozeronii and Helicobacter salomonis . The bacterium Candidatus Helicobacter heilmannii was cultured from feline gastric mucosa, which resulted in a now valid description of H. heilmannii as a novel species . Haesebrouck proposed the use of the name H. heilmannii sensu stricto ( s.s .)…”

Section: Benefits Vs Risks Of H Pylorimentioning

confidence: 99%

“…71 The bacterium Candidatus Helicobacter heilmannii 73 was cultured from feline gastric mucosa, which resulted in a now valid description of H. heilmannii as a novel species. 74 Haesebrouck proposed the use of the name H. heilmannii sensu stricto (s.s.) to be used for this species and H. heilmannii sensulato (s.l.) should refer to the whole group of NHPHs such that species designations are reserved for those situations in which the species is defined.…”

Section: Non-helicobacter Pylori Helicobactersmentioning

confidence: 99%

Review article: bacteria and pathogenesis of disease in the upper gastrointestinal tract – beyond the era of Helicobacter pylori

Walker

Talley

2014

Aliment Pharmacol Ther

View full text Add to dashboard Cite

Summary Background Study of the upper gastrointestinal microbiome has shown that other bacteria besides Helicobacter pylori flourish despite the hostile environment. Whilst H. pylori is the most studied bacteria in this region with a defined role in inflammation and neoplasia, it is apparent that other bacteria may contribute to UGI disease. Aim To review current knowledge of bacteria inhabiting the oesophagus, stomach and duodenum. Methods Published studies on the upper gastrointestinal microbiome (extracted from PubMed during the last 20 years). Results The stomach is a hostile environment for bacteria; however, recent studies categorising the microbiota have shown surprising results. Helicobacter pylori has been intensively studied since 1984 and recent sequencing analysis of other gastric microbiota shows that H. pylori is not alone. Composition can be influenced by acid suppression, gastritis and abundance of H. pylori. Eradication of H. pylori, whilst decreasing gastric cancer is associated with an increase in asthma, reflux and obesity. A future approach may be to selectively eradicate bacteria which predispose to inflammation and cancer as opposed to a comprehensive knockout policy. In the oesophagus, viridans streptococci are the most common bacteria influenced by both oral and gastric bacteria. Oesophagitis and Barrett's oesophagus are characterised by a significant decrease in Gram‐positive bacteria and an increase in Gram‐negative bacteria. An inverse association of H. pylori and oesophageal adenocarcinoma is described. The duodenal microbiome has been shown to influence small intestinal bacterial overgrowth, irritable bowel syndrome and coeliac disease. The numbers of bacteria recoverable by culture are variable in the stomach mucosa and gastric juice, typically 102–104 colony‐forming units (CFU)/g or mL and in the oesophagus, up to 104 bacteria per mm2 mucosal surface. In the small bowel, in health, 103 CFU/mL are normal. Conclusion This review highlights current knowledge of upper gastrointestinal bacteria and associations with disease.

show abstract

The Other Helicobacters

Cited by 13 publications

References 51 publications

Comparison of media and growth conditions for culturing enterohepaticHelicobacterspecies

Comparison of media and growth conditions for culturing enterohepaticHelicobacterspecies

Comparative Genomics ofH. pyloriand Non-PyloriHelicobacterSpecies to Identify New Regions Associated with Its Pathogenicity and Adaptability

Review article: bacteria and pathogenesis of disease in the upper gastrointestinal tract – beyond the era of Helicobacter pylori

Contact Info

Product

Resources

About