The overexpression of cytochromeP450genes confers buprofezin resistance in the brown planthopper,Nilaparvata lugens(Stål)

Abstract

Background Buprofezin, an insect growth regulator, has been widely used to control brown planthopper (BPH), Nilaparvata lugens, one of the most destructive pests of rice crops in Asia. The intensive use of this compound has resulted in very high levels of resistance to buprofezin in the field, however, the underpinning mechanisms of resistance have not been fully resolved. Results Insecticide bioassays using the P450 inhibitor piperonyl butoxide significantly synergized the toxicity of buprofezin in two resistant strains of BPH (BPR and YC2017) compared to a susceptible strain (Sus), suggesting P450s play a role in resistance to this compound. Whole transcriptome profiling identified 1110 genes that were upregulated in the BPR strain compared to the Sus strain, including 13 cytochrome P450 genes, eight esterases and one glutathione S‐transferase. Subsequently, qPCR validation revealed that four of the P450 genes, CYP6ER1vA, CYP6CW1, CYP4C77, and CYP439A1 were significantly overexpressed in both the BRP and YC2017 strains compared with the Sus strain. Further functional analyses showed that only suppression of CYP6ER1vA, CYP6CW1, and CYP439A1 gene expression by RNA interference significantly increased the toxicity of buprofezin against BPH. However, only transgenic Drosophila melanogaster expressing CYP6ER1vA and CYP439A1 exhibited significant resistance to buprofezin. Finally, the BPR strain was found to exhibit modest but significant levels of resistance to acetamiprid, dinotefuran and pymetrozine. Conclusions Our findings provide strong evidence that the overexpression of CYP6ER1vA and CYP439A1 contribute to buprofezin resistance in BPH, and that resistance to this compound is associated with low‐level resistance to acetamiprid, dinotefuran and pymetrozine. These results advance understanding of the molecular basis of BPH resistance to buprofezin and will inform the development of management strategies for the control of this highly damaging pest. © 2022 Society of Chemical Industry.

CRISPR/Cas9‐mediated knockout of NlCYP6CS1 gene reveals its role in detoxification of insecticides in Nilaparvata lugens (Hemiptera: Delphacidae)

“…Our results demonstrated that high expression of NlCYP6CS1 may be responsible for cross‐resistance between pymetrozine and neonicotinoids, which may also explain molecular mechanisms for the rapid development of pymetrozine resistance in the field 46 . However, knockout of NlCYP6CS1 did not affect the susceptibility of buprofezin, which was consistent with a recent study 47 . In this study, we also found no significant change in the susceptibility of the Nl6CS1 ‐KO strain to chlorpyrifos and triflumezopyrim.…”

“…46 However, knockout of NlCYP6CS1 did not affect the susceptibility of buprofezin, which was consistent with a recent study. 47 In this study, we also found no significant change in the susceptibility of the Nl6CS1-KO strain to chlorpyrifos and triflumezopyrim. Indeed, the field populations of N. lugens have not developed high resistance to chlorpyrifos and triflumezopyrim (Fig.…”

Molecular Evolutionary Mechanisms of CYP6ER1vA-Type Variant Associated with Resistance to Neonicotinoid Insecticides in Field Populations of Nilaparvata lugens

“…Duarte et al reported that CYP6ER1vA -type conferred cross-resistance to ethiprole, a phenylpyrazole insecticide that targets the gamma-aminobutyric acid-gated chloride channel in insects, with the mechanism due to the mutations occurring outside the SRS, rather than the T318S, A375del, and A376G mutations. In another recent study, transgenic D. melanogaster expressing the CYP6ER1vA -type exhibited significant resistance to buprofezin, suggesting the role of CYP6ER1vA -type in conferring resistance to buprofezin, an insect growth regulator.…”

CYP4CL2 Confers Metabolic Resistance to Pyridaben in the Citrus Pest Mite Panonychus citri

“…lugens have led to resistance to several neonicotinoids . The transgenic flies have been frequently applied in P450 function analysis when the active recombinant protein cannot be achieved, − which could provide evidence in P450-mediated resistance development in combination with the RNAi assay. Therefore, we introduced CYP4CL2 into Drosophila to verify the function in vitro .…”

“…melanogaster overexpressing two N. lugens P450 genes ( CYP6ER1vA and CYP439A1 ) exhibited significant resistance to buprofezin . Another study reported that the duplication and divergence of CYP6ER1 in N.…”