The Overexpression of Hypomethylated miR-663 Induces Chemotherapy Resistance in Human Breast Cancer Cells by Targeting Heparin Sulfate Proteoglycan 2 (HSPG2)

Hu, Haiyan; Li, Shuqin; Cui, Xiaona; Lv, Xiaobin; Jiao, Yu; Yu, Fang; Yao, Herui; Song, Erwei; Chen, Yongsong; Wang, Minghui; Lin, Ling

doi:10.1074/jbc.m112.434340

Cited by 110 publications

(76 citation statements)

References 24 publications

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“…miRNAs bind primarily to the 3'-untranslated region (3'UTR) of their target messenger RNAs (mRNAs) to reduce their stability and decrease the expression of target mRNAs at the post-transcriptional level (13), which play important roles in various biological processes including cell growth, proliferation, differentiation and death (14)(15)(16)(17)(18)(19). Concerning chemotherapy in various types of cancers such as breast cancer, lung adenocarcinoma, glioblastoma, and ovarian cancer, recent studies have shown that miRNAs also act in important roles (20)(21)(22)(23)(24). In ESCC, altered expression of miR-1290, miR-655, miR-375 and others, has been observed (25)(26)(27), suggesting that miRNA deregulation plays an important role in ESCC development.…”

Section: Introductionmentioning

confidence: 99%

miR-1291 targets mucin 1 inhibiting cell proliferation and invasion to promote cell apoptosis in esophageal squamous cell carcinoma

et al. 2015

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Abstract. MicroRNAs (miRNAs) are well known as important regulators in various cancer development. In the present study, we focused on the expression and biological function of miR-1291 in esophageal squamous cell carcinoma (ESCC). Compared with adjacent non-tumorous tissue samples, qRT-PCR data showed significant downregulation of miR-1291 in 54 ESCC tissue samples (P<0.05), which was also significantly associated with lymph node metastases and clinical stage (P<0.05). Cell Counting Kit-8 (CCK-8), colony formation, Transwell and flow cytometric apoptosis assays were performed to detect the effect of miR-1291 upregulation, and the results showed inhibition of the proliferation, invasion and promotion of apoptosis in EC9706 and EC-1 cells. Using bioinformatic analyses, we found that mucin 1 (MUC1) was a potential target for miR-1291. Luciferase assays were performed to reveal that miR-1291 inhibited MUC1 expression by targeting the seed region of MUC1 3'-untranslated region (3'UTR). We also found that the expression of MUC1 lacking in 3'UTR abrogated the anti-invasion and pro-apoptosis function of miR-1291. Our results demonstrated the importance of miR-1291 in targeting MUC1 for the regulation of esophagus cancer growth, invasion and apoptosis, and may be helpful for developing new targets for early diagnosis or new therapeutic targets for ESCC.

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Section: Introductionmentioning

confidence: 99%

miR-1291 targets mucin 1 inhibiting cell proliferation and invasion to promote cell apoptosis in esophageal squamous cell carcinoma

et al. 2015

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“…Microarray and qRT-PCR results all showed that upon exposure to PT, miR663b decreased dramatically in EC cells. We have previously described the oncogenic function of miR-663 in breast cancer [19] . Many studies reached the same conclusion.…”

Section: Discussionmentioning

confidence: 99%

“…Tissue in situ hybridization for miRNA detection In our previous paper, we used ISH to demonstrate that miR-663 is overexpressed in breast cancer [19] . Here, we evaluated mIR-663 levels in 51 cases of EC using tissue paraffin sections.…”

Section: Western Blot Assaymentioning

confidence: 99%

“…The PrimeScript miRNA RT-PCR kit (Takara, Dalian, China) was used to detect miR-663b and BCL214L levels as previously described [19] . The primers for BCL2L14 were forward, 5'-GCTCTGCTGTCTTCTCACCAAA-3' and reverse, 5'-ATTTTCCTCCTTCTCTGCTACTCC-3', and the β-actin primers were forward, 5′-TGAAGTGTGACGTGGACATC-3′ and reverse, 5′-GGAGGAGCAATGATCTTGAT-3′.…”

Section: Qrt-pcr Assaymentioning

confidence: 99%

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Pterostilbene suppresses human endometrial cancer cells in vitro by down-regulating miR-663b

Wang

Shen

et al. 2017

Acta Pharmacol Sin

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Resveratrol has long been known as an antioxidant and a chemopreventive agent. Similar to resveratrol, pterostilbene (PT) is also a phenolic compound extracted from the Vitis species. However, there are few studies on the antitumor effect of PT. Thus, we investigated the effects of PT on the endometrial cancer (EC) cells in vitro and the related molecular mechanisms. Treatment of EC cell lines HTB-111 and Ishikawa with PT (25-100 µmol/L) dose-dependently suppressed the cell viability and induced apoptosis. Using miR microarrays, we examined the miR expression profile in Ishikawa cells with or without PT, and revealed that miR-663b was the most decreased in PT-treated Ishikawa cells. Furthermore, we predicted and verified that the pro-apoptosis factor BCL2L14 is the direct target of miR-663b. Over-expression of miR-663b and knock-down of BCL2L14 counteracted the suppressing effects of PT on HTB-111 and Ishikawa cells. In addition, we evaluated the miR-663b levels in EC tissues of 51 patients using an in situ hybridization technique. With the median of the score of miR-663b as a cut-off value, these EC patients were divided into two groups, and the patients with high miR-663b expression had significantly poor prognosis.

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“…miRNAs have been revealed to be associated with cell chemosensitivity or chemotherapy resistance in a variety of cancer cell types, including ovarian and breast cancer cells (6)(7)(8). However, there is limited available data on the potential role of miRNAs in the chemotherapy resistance of gastric cancer.…”

Section: Introductionmentioning

confidence: 99%

Downregulation of microRNA-17-5p inhibits drug resistance of gastric cancer cells partially through targeting p21

Wang

2018

Oncol Lett

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Abstract. MicroRNAs (miRNAs/miRs) are endogenous small non-coding RNAs that post-transcriptionally regulate the expression of genes and serve crucial roles in diverse biological processes. The present study aimed to examine the miRNA expression profile and drug resistance in the SGC7901 cell line and its isogenic drug-resistant counterpart, SGC7901/cisplatin (DDP) cell line. The potential role of miR-17-5p in modulating drug resistance in gastric cancer cells was investigated. Different levels of miRNA expression between SGC7901/DDP and SGC7901 cells were analyzed by miRNA microarray and validated by quantitative polymerase chain reaction. It was indicated that the downregulation of miR-17-5p sensitized SGC7901/DDP cells to anticancer drugs. A decreased luciferase activity of p21 3'-untranslated region-based reporter in miR-17-5p-transfected SGC7901/DDP cells suggested that p21 may be a direct target gene of miR-17-5p. Western blot analysis and flow cytometric assay revealed that the downregulation of miR-17-5p increases the sensitivity of SGC7901/DDP cells to DDP-induced apoptosis. Taken together, these results demonstrated that miR-17-5p may perform a role in the development of drug resistance in gastric cancer cells, at least partially by modulating apoptosis via targeting p21. IntroductionGastric cancer is one of the major causes of mortality worldwide (1). Currently, the standard treatment regimen for gastric cancer includes surgery, chemotherapy and radiotherapy (2). In patients at advanced stages, chemotherapy has been demonstrated to improve survival, by preventing tumor invasion or downsizing distant metastatic lesions (3). One of the leading causes of chemotherapy failure in gastric cancer is tumor resistance. Patients who are less responsive to chemotherapy have a poorer prognosis (4). Thus, it is important to identify the molecular mechanisms underlying the drug resistance of gastric cancer cells.MicroRNAs (miRNAs/miRs) are a family of endogenous non-coding RNA molecules that can post-transcriptionally regulate gene expression and have been shown to perform crucial roles in diverse biological processes, including apoptosis, proliferation, stress response and metabolism (5). miRNAs have been revealed to be associated with cell chemosensitivity or chemotherapy resistance in a variety of cancer cell types, including ovarian and breast cancer cells (6-8). However, there is limited available data on the potential role of miRNAs in the chemotherapy resistance of gastric cancer.The present study reported that miR-17-5p was upregulated in the multidrug-resistant human gastric cancer SGC7901/cisplatin (DDP) cell line, compared with the parental SGC7901 cell line. It was demonstrated that the downregulation of miR-17-5p was able to inhibit drug resistance and increase DDP-induced apoptosis. In addition, it was indicated that p21 was upregulated in miR-17-5p-transfected cells compared with cells transfected with control miRNA inhibitor. These results demonstrated that miR-17-5p may perform a role in the develop...

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The Overexpression of Hypomethylated miR-663 Induces Chemotherapy Resistance in Human Breast Cancer Cells by Targeting Heparin Sulfate Proteoglycan 2 (HSPG2)

Cited by 110 publications

References 24 publications

miR-1291 targets mucin 1 inhibiting cell proliferation and invasion to promote cell apoptosis in esophageal squamous cell carcinoma

miR-1291 targets mucin 1 inhibiting cell proliferation and invasion to promote cell apoptosis in esophageal squamous cell carcinoma

Pterostilbene suppresses human endometrial cancer cells in vitro by down-regulating miR-663b

Downregulation of microRNA-17-5p inhibits drug resistance of gastric cancer cells partially through targeting p21

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