The oxidation of α-olefins by aPseudomonas reactions involving the double bond

Metabolism of ethylene by Mycobacterium E 20

“…Enzymes of this class catalyse the ring-opening of epoxides with glutathion to give corresponding thioethers, or with water to give a diol. Non-enzymic hydrolysis of epoxides may also occur [13]. The possibility that ethylene glycol was an intermediate in ethylene oxide metabolism in Mycobacterium E 20 had become unlikely from previous experiments [3].…”

Characterization of hydrocarbon emulsification and solubilization occurring during the growth of Endomycopsis lipolytica on hydrocarbons

“…It is known that growth of this yeast on hexadecene-1 is biphasic,12 the first growth phase depending on the utilization of the alkene and the second phase probably on the utilization of diol metabolite of hexadecene. 23 The result indicates that hexadecene solubilizing capacity declined when the cells started growing on substrate other than hexadecene. Table I shows that the maximum solubility of various n-alkanes and alkenes in aqueous culture medium during the cultivation of the yeast on these hydrocarbons was increased many lOOOfolds as compared to the normal aqueous solubility of these hydrocarbons.…”

Epoxidation of short chain alkenes in Mycobacterium E20: The involvement of a specific mono-oxygenase

“…All of these organisms contain a mono-oxygenase system which oxidizes the n-alkane by the incorporation of one atom of oxygen into the substrate whilst the other oxygen atom is reduced to water. Although such organisms are generally not able to grow on n-alkanes, they nevertheless are able to oxidize these compounds either by the hydroxylation of a methyl group or more commonly, by the epoxidation of the double bond [4][5][6][7][8][9][10]. For example the methane mono-oxygenase from Methylococcus capsulatus (Bath) is very non-specific and is able to hydroxylate n-alkanes, cyclic alkanes and aromatic compounds, whilst catalyzing the oxidation of terminal alkenes to yield the corresponding 1-2 epoxides [11].…”