The Oxidative Effect of Prolonged CO2 Pneumoperitoneum A Comparative Study in Rats

Kontoulis, Theodoros M.; Pissas, Dimitrios; Pavlidis, Theodosios; Pissas, Georgios; Lalountas, Miltiadis; Κολιάκος, Γεώργιος; Topouridou, Konstantina; Sakantamis, Athanasios K.

doi:10.1016/j.jss.2011.09.030

Cited by 16 publications

(22 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Uysal et al reported that flap necrosis associates with high MPO levels and they stated that inflammatory response and MPO levels significantly decrease in DEX administration group [7]. Kontoulis et al showed that Pp induced I/R injury causes higher MPO levels and also stated that long duration of Pp and reperfusion causes more increseasing in MPO levels [36]. MPO activities in our study were measured in the blood samples that were taken 60 minutes after Pp.…”

Section: Resultsmentioning

confidence: 58%

The effects of Dexmedetomidine on oxidant - antioxidant systems in the experimental model of carbondioxide pneumoperitoneum

Taş¹

2014

Turk J Bioch

View full text Add to dashboard Cite

Objective: The aim of the study was to investigate the changes of oxidative and anti-oxidative systems in the splanchnic area during carbon dioxide pneumoperitoneum and to determine whether the administration of dexmedetomidine has effects on these systems. Methods: Forty rats were randomized into four groups: Group I; Control, Group II; No pneumoperitoneum, Dexmedetomidine administration, Group III; Pneumoperitoneum, no Dexmedetomidine administration and Group IV; Pneumoperitoneum and Dexmedetomidine administration 30 minutes before insufflation. The rats were rested 30 minutes after desufflation and blood samples were obtained for; ischaemia modified albumin (IMA), myeloperoxidase (MPO), advanced oxidation protein products (AOPP), catalase (CAT), paraoxonase (PON1) and platelet-activating factor acetylhydrolase (PAF-AH) analyses. Results: When compared with the control group; the serum IMA levels significantly decreased in group II, and also increased in group III as compared to control (p<0.05). IMA levels were also significantly decreased in both groups II and IV as compared to group III (p<0.001). Serum MPO activity increased in group III as compared to control (p<0.05). Serum AOPP levels were significantly increased in group III as compared to group II (p<0.01) and decreased in group IV as compared to group III (p<0.01). Serum CAT activity was higher in group II than controls (p<0.05). Serum PON and plasma PAF-AH activities significantly decreased in grup III as compared to group II (p<0.05) and plasma PAF-AH activity were decreased in group III as compared to controls (p<0.05). Conclusion:In conclusion, administration of dexmedetomidine; prior to ischemia reperfusion injury caused by pneumoperitoneum; reduces the oxidative injury and increases the antioxidant activity in the acute period.

show abstract

Section: Resultsmentioning

confidence: 58%

The effects of Dexmedetomidine on oxidant - antioxidant systems in the experimental model of carbondioxide pneumoperitoneum

Taş¹

2014

Turk J Bioch

View full text Add to dashboard Cite

show abstract

“…e amount of lung damage was also lowest in the KP2 group. Total damage was calculated as the sum of total of the scores for each of the intracellular edema, congestion, interstitial hemorrhage, and in ammatory cell in ltration parameters of the histologically examined [1].…”

Section: Resultsmentioning

confidence: 99%

“…A pathologist, who was blinded to the study group assignment, examined the sections under light microscopy for tissue injury. e histologic scoring system described by Hauet et al [1,14] was used (Table 1a). Each section was assessed for in ammatory cell in ltration, cellular edema, congestion and hemorrhage.…”

Section: Histopathologic Examinationmentioning

confidence: 99%

“…As a result of increased expertise in technically demanding procedures and improvements in instrumentation, laparoscopic surgery has become an integral part of modern-day surgical practice [1]. Despite the general safety of the procedure, ischemia and other adverse e ects may occur a er routine laparoscopy, even in young, healthy patients with no preoperative complications [2,3].…”

mentioning

confidence: 99%

See 1 more Smart Citation

Low-dose ketamine pretreatment reduces oxidative damage and inflammatory response following CO2 pneumoperitoneum in rats

Xu¹,

Gào²,

Peng³

et al. 2014

CIM

View full text Add to dashboard Cite

Low-dose ketamine pretreatment reduces oxidative damage and in ammatory response following CO2 pneumoperitoneum in rats AbstractPurpose: e duration of pneumoperitoneum during laparoscopic procedures may contribute to post-surgical oxidative stress. Previous studies have shown that low-dose ketamine, an anesthetic with anti-in ammatory properties, protects various organs from ischemia-reperfusion injury. is study investigated the e ects of low-dose ketamine on the overproduction of oxidants and the tissue damage caused by intra-abdominal pressure during CO2 pneumoperitoneum.Methods: Male Sprague Dawley rats received a CO2 pneumoperitoneum of 15 mmHg and preceded by either low-dose ketamine (KP1, 5 mg/kg; KP2, 10 mg/kg) or 0.9% saline (PR, 3 ml). General anethesia was provided by pentobarbital and sevo urane. e control group (CR) received an intraperitoneal saline injection and sham surgery. ree hours a er pneumoperitoneum, serum concentrations of interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-α), malondialdehyde (MDA), superoxide dismutase (SOD) and intestinal fatty acid binding protein (iFABP) were measured and liver, kidney, lung, and intestine were evaluated for tissue damage.Results: e highest plasma MDA, TNF-α, IL-6 and iFABP values were observed at T1 (a er 3 hours of pneumoperitoneum) in the PR group, followed by the KP1, KP2, and CR groups (P < 0.01). SOD concentrations showed an opposite trend and were highest in the CR group, followed by the KP2, KP1, and PR groups (P < 0.01). TNF-α concentration was signi cantly lower in the KP2 than the KP1 group (P < 0.05). Histopathologic scoring of organ sections demonstrated the lowest scores in the KP2 group, followed by the KP1 and PR groups, in an increasing order (P < 0.05).Conclusion: Pretreatment with low-dose ketamine before general anaesthesia protects against potential oxidative damage and in ammatory response caused by CO2 pneumoperitoneum.

show abstract

“…The effects of intraabdominal pressure during pneumoperitoneum have been reported in rat and pig animal models [9,13,14,15,16]. These reports were based on normal kidneys.…”

Section: Discussionmentioning

confidence: 99%

Acute Kidney Injury Induced by Various Pneumoperitoneum Pressures in a Rabbit Model of Mild and Severe Hydronephrosis

Li¹,

Cao

Meng³

et al. 2014

Urol Int

View full text Add to dashboard Cite

Objective: Increased pneumoperitoneum pressure during laparoscopic surgery can result in acute kidney injury. We aimed to clarify whether intraabdominal pressure tolerance is modified in various degrees of unilateral kidney hydronephrosis in rabbits. Methods: A total 90 rabbits were randomly allocated to three groups (group PN, PM and PS, i.e. rabbits with no, mild and severe hydronephrosis, respectively, subjected to intraabdominal pressures). Rabbits in group PM (n = 30) and group PS (n = 30) underwent a surgical procedure inducing a mild or severe left hydronephrosis. Rabbits in all groups were then allocated to 5 subgroups. Then, they were subjected to intraabdominal pressures of 0, 6, 9, 12, and 15 mm Hg, respectively. Acute kidney injury was assessed by measuring serum creatinine (Scr), blood urea nitrogen (BUN), tubular cell apoptosis, kidney injury molecule-1 (KIM-1) and cysteine-rich 61 (Cyr-61/CCN1) expression. Results: Acute kidney injury with increased tubular apoptosis and KIM-1 and Cyr-61 expression occurred when intraabdominal pressure reached 15, 15 and 9 mm Hg in PN, PM and PS groups, respectively. The Scr and BUN levels were similar in all groups. Conclusions: In rabbits, kidneys with severe hydronephrosis were more likely to suffer acute injury when they were exposed to pneumoperitoneal pressure.

show abstract

The Oxidative Effect of Prolonged CO2 Pneumoperitoneum A Comparative Study in Rats

Cited by 16 publications

References 32 publications

The effects of Dexmedetomidine on oxidant - antioxidant systems in the experimental model of carbondioxide pneumoperitoneum

The effects of Dexmedetomidine on oxidant - antioxidant systems in the experimental model of carbondioxide pneumoperitoneum

Low-dose ketamine pretreatment reduces oxidative damage and inflammatory response following CO2 pneumoperitoneum in rats

Acute Kidney Injury Induced by Various Pneumoperitoneum Pressures in a Rabbit Model of Mild and Severe Hydronephrosis

Contact Info

Product

Resources

About