The Oxidative Response of Mouse Hearts is Modulated by Genetic Background

Silva, Marco Aurélio dos Santos; Nagato, Akinori Cardozo; Trajano, Eduardo Tavares Lima; Alves, Jackson Nogueira; Bandeira, Ana Carla Balthar; Pôrto, Luís Cristóvão; Bezerra, Frank Silva

doi:10.5935/abc.20130029

Cited by 13 publications

(8 citation statements)

References 2 publications

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“…Our results with chloroform corroborate the findings of previous studies in which the exposure of target organs to toxic substances such as cigarette smoke and formaldehyde caused an influx of macrophages and polymorphonuclear cells by inducing oxidative stress. 18,38,39 The total cell counts in the BALF fluid of male mice corroborated results published by Templin et al, 9 Golden et al, 10 and Wolf and Butterworth. 40 The presence of chloroform in the lung parenchyma in mice in both the FEG and the MEG induced an inflammatory response.…”

Section: Discussionsupporting

confidence: 91%

Influence of Sexual Dimorphism on Pulmonary Inflammatory Response in Adult Mice Exposed to Chloroform

et al. 2015

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Chloroform is an organic solvent used as an intermediate in the synthesis of various fluorocarbons. Despite its widespread use in industry and agriculture, exposure to chloroform can cause illnesses such as cancer, especially in the liver and kidneys. The aim of the study was to analyze the effects of chloroform on redox imbalance and pulmonary inflammatory response in adult C57BL/6 mice. Forty animals were divided into 4 groups (N = 10): female (FCG) and male (MCG) controls, and females (FEG) and males (MEG) exposed to chloroform (7.0 ppm) 3 times/d for 20 minutes for 5 days. Total and differential cell counts, oxidative damage analysis, and protein carbonyl and antioxidant enzyme catalase (CAT) activity measurements were performed. Morphometric analyses included alveolar area (Aa) and volume density of alveolar septa (Vv) measurements. Compared to FCG and MCG, inflammatory cell influx, oxidative damage to lipids and proteins, and CAT activity were higher in FEG and MEG, respectively. Oxidative damage and enzyme CAT activity were higher in FEG than in FCG. The Aa was higher in FEG and MEG than in FCG and MCG, respectively. The Vv was lower in FEG and MEG than in FCG and MCG, respectively. This study highlights the risks of occupational chloroform exposure at low concentrations and the intensity of oxidative damage related to gender. The results validate a model of acute exposure that provides cellular and biochemical data through short-term exposure to chloroform.

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Section: Discussionsupporting

confidence: 91%

Influence of Sexual Dimorphism on Pulmonary Inflammatory Response in Adult Mice Exposed to Chloroform

et al. 2015

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“…The supernatant was collected and the final volume of all samples adjusted to 1.5 mL with phosphate buffer. The samples were stored in a freezer for later biochemical analysis (24). …”

Section: Methodsmentioning

confidence: 99%

Time-course effects of aerobic exercise training on cardiovascular and renal parameters in 2K1C renovascular hypertensive rats

Maia

Sousa

Santos

et al. 2015

Braz J Med Biol Res

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Exercise training (Ex) has been recommended for its beneficial effects in hypertensive states. The present study evaluated the time-course effects of Ex without workload on mean arterial pressure (MAP), reflex bradycardia, cardiac and renal histology, and oxidative stress in two-kidney, one-clip (2K1C) hypertensive rats. Male Fischer rats (10 weeks old; 150–180 g) underwent surgery (2K1C or SHAM) and were subsequently divided into a sedentary (SED) group and Ex group (swimming 1 h/day, 5 days/week for 2, 4, 6, 8, or 10 weeks). Until week 4, Ex decreased MAP, increased reflex bradycardia, prevented concentric hypertrophy, reduced collagen deposition in the myocardium and kidneys, decreased the level of thiobarbituric acid-reactive substances (TBARS) in the left ventricle, and increased the catalase (CAT) activity in the left ventricle and both kidneys. From week 6 to week 10, however, MAP and reflex bradycardia in 2K1C Ex rats became similar to those in 2K1C SED rats. Ex effectively reduced heart rate and prevented collagen deposition in the heart and both kidneys up to week 10, and restored the level of TBARS in the left ventricle and clipped kidney and the CAT activity in both kidneys until week 8. Ex without workload for 10 weeks in 2K1C rats provided distinct beneficial effects. The early effects of Ex on cardiovascular function included reversing MAP and reflex bradycardia. The later effects of Ex included preventing structural alterations in the heart and kidney by decreasing oxidative stress and reducing injuries in these organs during hypertension.

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“…C57BL6 male mice (n = 36) were exposed to 6 commercial fullflavor filtered Virginia cigarettes (10 mg of tar, 0.9 mg of nicotine and 10 mg of carbon monoxide) per day for 5 days by using a smoking chamber previously described [20] [21]. The groups exposed to CS for 1, 2, 3, 4 or 5 days were called CSD1, CSD2, CSD3, CSD4, and CSD5, respectively.…”

Section: Cigarette Smoke Exposure Protocolmentioning

confidence: 99%

Temporal analysis of oxidative effects on the pulmonary inflammatory response in mice exposed to cigarette smoke

Campos

Manso

Gonçalves

et al. 2013

Cellular Immunology

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The Oxidative Response of Mouse Hearts is Modulated by Genetic Background

Cited by 13 publications

References 2 publications

Influence of Sexual Dimorphism on Pulmonary Inflammatory Response in Adult Mice Exposed to Chloroform

Influence of Sexual Dimorphism on Pulmonary Inflammatory Response in Adult Mice Exposed to Chloroform

Time-course effects of aerobic exercise training on cardiovascular and renal parameters in 2K1C renovascular hypertensive rats

Temporal analysis of oxidative effects on the pulmonary inflammatory response in mice exposed to cigarette smoke

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