The Panax ginseng PgTIP1 gene confers enhanced salt and drought tolerance to transgenic soybean plants by maintaining homeostasis of water, salt ions and ROS

An, Jinpeng; Cheng, Cong; Hu, Zhenmin; Chen, Haiying; Cai, Weiming; Yu, Bingjun

doi:10.1016/j.envexpbot.2018.06.025

Cited by 40 publications

(25 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is probably that the flow of water mediated by PIP1 or PIP2 towards the cytoplasm and by TIPs towards the vacuole in tolerant genotype is efficiently conducted for its compartmentalization. This hypothesis agrees with studies of PIP1 from Salicornia bigelovii or AQP7 from wheat expressed in Nicotiana tabacum , PgTIP1 from Panax ginseng in Glycine max and SlPIP2-7 from S. lycopersicum or JcPIP2-7 from Jatropha curcas in A. thaliana [ 25 , 32 , 92 , 93 , 94 ]. However, there is no total consensus about the physiological mechanisms associated with the function of AQP genes and how and/or why these genes are up or down-regulated in plants under the same water stress [ 95 ].…”

Section: Discussionsupporting

confidence: 91%

Genome-Wide Identification and Transcriptional Regulation of Aquaporin Genes in Bread Wheat (Triticum aestivum L.) under Water Stress

Madrid-Espinoza

Brunel-Saldias

Guerra

et al. 2018

Genes

View full text Add to dashboard Cite

Aquaporins (AQPs) are transmembrane proteins essential for controlling the flow of water and other molecules required for development and stress tolerance in plants, including important crop species such as wheat (Triticum aestivum). In this study, we utilized a genomic approach for analyzing the information about AQPs available in public databases to characterize their structure and function. Furthermore, we validated the expression of a suite of AQP genes, at the transcriptional level, including accessions with contrasting responses to drought, different organs and water stress levels. We found 65 new AQP genes, from which 60% are copies expanded by polyploidization. Sequence analysis of the AQP genes showed that the purifying selection pressure acted on duplicate genes, which was related to a high conservation of the functions. This situation contrasted with the expression patterns observed for different organs, developmental stages or genotypes under water deficit conditions, which indicated functional divergence at transcription. Expression analyses on contrasting genotypes showed high gene transcription from Tonoplast Intrinsic Protein 1 (TIP1) and 2 (TIP2), and Plasma Membrane Intrinsic Protein 1 (PIP1) and 2 (PIP2) subfamilies in roots and from TIP1 and PIP1 subfamilies in leaves. Interestingly, during severe drought stress, 4 TIP genes analyzed in leaves of the tolerant accession reached up to 15-fold the level observed at the susceptible genotype, suggesting a positive relationship with drought tolerance. The obtained results extend our understanding of the structure and function of AQPs, particularly under water stress conditions.

show abstract

Section: Discussionsupporting

confidence: 91%

Genome-Wide Identification and Transcriptional Regulation of Aquaporin Genes in Bread Wheat (Triticum aestivum L.) under Water Stress

Madrid-Espinoza

Brunel-Saldias

Guerra

et al. 2018

Genes

View full text Add to dashboard Cite

show abstract

“…Li et al [ 179 ] observed that overexpression of GmNAC15 in soybean hairy roots improve SS tolerance. An et al [ 180 ] suggested that PgTIP1 is an SS tolerance associated gene involved in improving SS tolerance in transgenic soybean lines. Overexpression of GmBIN2 enhances SS tolerance in soybean and Arabidopsis [ 181 ].…”

Section: Management Strategies To Improve Salt Tolerance In Legumementioning

confidence: 99%

Grain Legumes and Fear of Salt Stress: Focus on Mechanisms and Management Strategies

Nadeem

Yahya

et al. 2019

IJMS

View full text Add to dashboard Cite

Salinity is an ever-present major constraint and a major threat to legume crops, particularly in areas with irrigated agriculture. Legumes demonstrate high sensitivity, especially during vegetative and reproductive phases. This review gives an overview of legumes sensitivity to salt stress (SS) and mechanisms to cope with salinity stress under unfavorable conditions. It also focuses on the promising management approaches, i.e., agronomic practices, breeding approaches, and genome editing techniques to improve performance of legumes under SS. Now, the onus is on researchers to comprehend the plants physiological and molecular mechanisms, in addition to various responses as part of their stress tolerance strategy. Due to their ability to fix biological nitrogen, high protein contents, dietary fiber, and essential mineral contents, legumes have become a fascinating group of plants. There is an immense need to develop SS tolerant legume varieties to meet growing demand of protein worldwide. This review covering crucial areas ranging from effects, mechanisms, and management strategies, may elucidate further the ways to develop SS-tolerant varieties and to produce legume crops in unfavorable environments.

show abstract

“…Similarly, MicroRNA156 has been found to improve drought stress tolerance in alfalfa ( Arshad et al, 2017 ) and heat stress ( Matthews et al, 2018 ) by silencing SPL13. Conversely, biotechnological improvement of soybeans has been reported for genes such as a L -Δ 1-Pyrroline-5-carboxylate reductase ( De Ronde et al, 2000 , 2004 ) conferring improved drought tolerance, and the Panax ginseng PgTIP1 gene conferring enhanced salt and drought tolerance ( An et al, 2018 ). The recent progress in functional genomics and in understanding the mechanisms of abiotic stress tolerance in model crop like Arabidopsis ( A. thaliana ) and rice ( Oryza sativa ) has opened a way for genetic manipulation of soybean plants with abiotic stress tolerance ( Thao and Tran, 2012 ).…”

Section: Impact Of Climate Change On Legume Forage Qualitymentioning

confidence: 99%

Harnessing the Potential of Forage Legumes, Alfalfa, Soybean, and Cowpea for Sustainable Agriculture and Global Food Security

et al. 2018

View full text Add to dashboard Cite

Substantial improvements in access to food and increased purchasing power are driving many people toward consuming nutrition-rich foods causing an unprecedented demand for protein food worldwide, which is expected to rise further. Forage legumes form an important source of feed for livestock and have potential to provide a sustainable solution for food and protein security. Currently, alfalfa is a commercially grown source of forage and feed in many countries. However, soybean and cowpea also have the potential to provide quality forage and fodder for animal use. The cultivation of forage legumes is under threat from changing climatic conditions, indicating the need for breeding cultivars that can sustain and acclimatize to the negative effects of climate change. Recent progress in genetic and genomic tools have facilitated the identification of quantitative trait loci and genes/alleles that can aid in developing forage cultivars through genomics-assisted breeding. Furthermore, transgenic technology can be utilized to manipulate the genetic makeup of plants to improve forage digestibility for better animal performance. In this article, we assess the genetic potential of three important legume crops, alfalfa, soybean, and cowpea in supplying quality fodder and feed for livestock. In addition, we examine the impact of climate change on forage quality and discuss efforts made in enhancing the adaptation of the plant to the abiotic stress conditions. Subsequently, we suggest the application of integrative approaches to achieve adequate forage production amid the unpredictable climatic conditions.

show abstract

The Panax ginseng PgTIP1 gene confers enhanced salt and drought tolerance to transgenic soybean plants by maintaining homeostasis of water, salt ions and ROS

Cited by 40 publications

References 43 publications

Genome-Wide Identification and Transcriptional Regulation of Aquaporin Genes in Bread Wheat (Triticum aestivum L.) under Water Stress

Genome-Wide Identification and Transcriptional Regulation of Aquaporin Genes in Bread Wheat (Triticum aestivum L.) under Water Stress

Grain Legumes and Fear of Salt Stress: Focus on Mechanisms and Management Strategies

Harnessing the Potential of Forage Legumes, Alfalfa, Soybean, and Cowpea for Sustainable Agriculture and Global Food Security

Contact Info

Product

Resources

About