The pap1+ gene of fission yeast is transcriptionally regulated by nitrosative and nutritional stress

Abstract

In the current work, regulation of the pap1(+) gene was investigated by the use of the pap1(+)-lacZ fusion gene and semi-quantitative reverse transcriptase-PCR. The synthesis of beta-galactosidase from the pap1(+)-lacZ fusion gene was significantly enhanced by nitric oxide (NO)-generating sodium nitroprusside (SNP) and nitrogen starvation. However, the induction by SNP and nitrogen starvation was observed to be much less in the Pap1p-negative cells harboring the fusion gene. Exogenous NO was more effectively scavenged in the Pap1p-positive cells than in the Pap1p-negative cells. Oxidative stress such as superoxide anion, hydrogen peroxide and cadmium could not give rise to an effect on the synthesis of beta-galactosidase from the fusion gene. The pap1(+) mRNA level was elevated in the wild-type cells by SNP and nitrogen starvation. Catalase activity, a major enzyme positively regulated by Pap1p, was significantly increased only in the Pap1p-positive cells by SNP. In brief, it is demonstrated that transcription of the Schizosaccharomyces pombe pap1(+) gene is positively regulated by nitrosative and nutritional stress in a Pap1p-dependent manner.

Oxidative Damage Induced by Heat Stress Could be Relieved by Nitric Oxide in Trichoderma harzianum LTR-2

“…Kong et al [7] reported that fungal cells produce endogenous NO that plays an important biological role in fungal growth or morphogenesis [4]. NO is directly involved in the regulation of gene transcription [8][9][10][11], cellular oxidative stress, thiobarbituric acid reactive substance (TBARS) production, protein Snitrosylation modification, tyrosine nitration, and trehalose accumulation [7,10,12]. These biological mechanisms are essential for maintaining fungal cell physiological activities.…”

Nitric oxide and nitrosative stress tolerance in yeast

“…Instead, Sarver and DeRisi have shown that the C 2 H 2 zinc finger transcription factor ScFZF1 is involved in NO sensing in S. cerevisiae [46]. In S. pombe, the AP-1-like bZIP transcription factor, SpPap1, regulates nitrosative as well as oxidative and nutritional stress responses [51]. The orthologue of SpPap1, ScYap1, regulates the oxidative stress response in S. cerevisiae .…”