The participation of electrolytes in adaptation mechanisms of intertidal molluscs' cells to altered salinity

IuV, Natochin; Berger, Vérane; Khlebovich, V. V.; Ea, Lavrova; Michailova, O.Yu

doi:10.1016/0300-9629(79)90636-4

Cited by 22 publications

(14 citation statements)

References 2 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…At moderately lowered salinity, intracellular [K + ] and [Na + ] are kept relatively stable at about 200 and 100 mM, respectively, but [K + ] drops rapidly under strong hypoosmotic stress to avoid cell swelling (Willmer, 1978;Wright et al, 1989;Silva and Wright, 1994). In order to stay isoosmotic with their environment following long-term acclimation to lowered salinity, intracellular [K + ] and [Na + ] are maintained at lower concentrations (Willmer, 1978;Natochin et al, 1979). In addition, bivalves reduce the concentration of intracellular compati-ble organic osmolytes, such as certain amino acids, taurine and betaine during the acclimation phase (Silva and Wright, 1994;Kube et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Calcification in a marginal sea – influence of seawater [Ca<sup>2+</sup>] and carbonate chemistry on bivalve shell formation

et al. 2018

View full text Add to dashboard Cite

Abstract. In estuarine coastal systems such as the Baltic Sea, mussels suffer from low salinity which limits their distribution. Anthropogenic climate change is expected to cause further desalination which will lead to local extinctions of mussels in the low saline areas. It is commonly accepted that mussel distribution is limited by osmotic stress. However, along the salinity gradient, environmental conditions for biomineralization are successively becoming more adverse as a result of reduced [Ca2+] and dissolved inorganic carbon (CT) availability. In larvae, calcification is an essential process starting during early development with formation of the prodissoconch I (PD I) shell, which is completed under optimal conditions within 2 days. Experimental manipulations of seawater [Ca2+] start to impair PD I formation in Mytilus larvae at concentrations below 3 mM, which corresponds to conditions present in the Baltic at salinities below 8 g kg−1. In addition, lowering dissolved inorganic carbon to critical concentrations (< 1 mM) similarly affected PD I size, which was well correlated with calculated ΩAragonite and [Ca2+][HCO3-] ∕ [H+] in all treatments. Comparing results for larvae from the western Baltic with a population from the central Baltic revealed a significantly higher tolerance of PD I formation to lowered [Ca2+] and [Ca2+][HCO3-] ∕ [H+] in the low saline adapted population. This may result from genetic adaptation to the more adverse environmental conditions prevailing in the low saline areas of the Baltic. The combined effects of lowered [Ca2+] and adverse carbonate chemistry represent major limiting factors for bivalve calcification and can thereby contribute to distribution limits of mussels in the Baltic Sea.

show abstract

Section: Introductionmentioning

confidence: 99%

Calcification in a marginal sea – influence of seawater [Ca<sup>2+</sup>] and carbonate chemistry on bivalve shell formation

et al. 2018

View full text Add to dashboard Cite

show abstract

“…In marine molluscs, these adaptations involve isoosmotic cell volume regulation through, for example, shifts of intracellular free amino acid and inorganic ion concentrations (e.g. Lockwood 1976;Natochin et al 1979;Taylor and Andrews 1988;Hawkins and Hilbish 1992), and changes of oxygen consumption rates during salinity acclimation (Kinne 1971;Berger 1986). Capacity adaptations typically result in adjustments which ensure performance of the most important life functions and potentially unlimited survival in the new osmotic environment.…”

Section: Introductionmentioning

confidence: 99%

Resistance to freshwater exposure in White Sea Littorina spp. I: Anaerobic metabolism and energetics

Sokolova

Bock²,

Pörtner³

2000

Journal of Comparative Physiology B: Biochemical, Systemic, and

View full text Add to dashboard Cite

Anaerobic metabolism and changes in the osmotic concentration of extravisceral¯uid were studied in the White Sea periwinkles (Littorina littorea, Littorina saxatilis and Littorina obtusata) during freshwater exposure. Resistance to hypoosmotic stress increased in the order: L. obtusata < L. saxatilis < L. littorea. Our data suggest that osmotic shock is not a primary reason for mortality of the periwinkles under these conditions. During environmental anaerobiosis, considerable succinate accumulation (up to 10±19 lmol g)1 wet weight), and depletion of phosphagen and ATP pools were found in the studied species. Other metabolic end products (alanopine, strombine, lactate, acetate or propionate) were not detected. Succinate accumulation and net ATP breakdown were the fastest in the least resistant species, L. obtusata, and slowest in the most resistant, L. littorea. Rate of ATP turnover decreased during freshwater exposure in L. littorea and L. saxatilis, but not in L. obtusata. Our data suggest that dierential resistance of three studied Littorina spp. to extreme hypoosmotic stress may be related to their dierent abilities to reduce metabolic rate and ATP turnover during sustained anoxia. Species-speci®c variations in anaerobic capacity of Littorina spp. are discussed in relation to their vertical distribution, size and ecology.Key words Salinity stress á Resistance adaptations á Anaerobic metabolism á ATP turnover á Littorina spp.Abbreviations ADP adenosine-5¢-diphosphate á AEC adenylate energy charge á AMP adenosine-5¢-monophosphate á ATP adenosine-5¢-triphosphate á Arg L-arginine á AWI Alfred-Wegener-Institute for Polar and Marine Research á dG/dn Gibbs free energy change á EDTA ethylenediaminetetraacetic acid á EF extravisceral¯uid á HPLC High Performance Liquid Chromatography á HSD honestly signi®cant dierence á w ATP ATP turnover rate á NTA nitrilotriacetic acid á PCA perchloric acid á P i inorganic phosphate á PLA phospho-L-arginine á R PLA relative amount of phosphagen á R ST standardised respiration rate

show abstract

“…Intracellular changes of Na + concentration affect the osmotic pressure response of organism, seal the mantle cavity, and protect cells from extreme salinity (Berger and Kharazova, 1997). Natochin et al (1979) studied Mytilus edulis and Littorina littorea and found that, in addition to Na + and K + pumps, these organisms also have a Na + exchange system related to Cl − that regulates the cell volume and osmotic pressure. In this sense, the Na + -K + pump and the Na + -Cl − pump might jointly be involved enabling marine shellfish to cope with salinity changes.…”

Section: Discussionmentioning

confidence: 99%

Effects of Sudden Drop in Salinity on Osmotic Pressure Regulation and Antioxidant Defense Mechanism of Scapharca subcrenata

Zhang

Liu

et al. 2020

Front. Physiol.

View full text Add to dashboard Cite

Salinity is an important ecological factor that impacts the growth and survival of aquatic organisms. The salinity of seawater in coastal and estuarine areas is often subject to dynamic changes because of seasonal rainfall and continental runoff. Thus, the current study investigated the effects of sudden changes in salinity on the survival rate and osmotic pressure regulation mechanisms of bottom-sowing seedlings of the economically important ark shell, Scapharca subcrenata. By simulating the sudden changes that occur in seawater salinity after rainstorms, the results showed that the osmotic pressure of the hemolymph and Na + , K + , Ca 2+ , and Cl − concentrations first decreased and then increased. When the salinity decreased from 30 to 14 , hemoglobin, soluble total protein, taurine, and total free amino acid gradually increased; maximum levels of hemoglobin, soluble total protein, and taurine occurred once the salinity increased to 22 at 96 h. After 96 h, the total free amino acid content increased until 144 h. The reactive oxygen species (ROS) content and total antioxidant capacity (T-AOC) peaked at 96 h, whereas the expression levels of Mn-superoxide dismutase (MnSOD) and catalase (CAT) increased earlier, indicating that, with continuous ROS generation, antioxidant defense mechanisms were activated to avoid oxidative damage. Expression levels of cathepsin C (CTSC), cathepsin D (CTSD), heat shock protein 20 (HSP20), and heat shock protein 70 (HSP70) were significantly higher than in the control group at 48 h (salinity level 14); the expression levels of HSP20, heat shock protein 90 (HSP90), MnSOD, and glutathione peroxidase (GP x) remained high, indicating that they were still required for osmotic pressure regulation to maintain the dynamic balance between the generation and removal of ROS as the salinity level increased. These results not only add to our basic understanding of the aquatic ecology of S. subcrenata, but also provide a theoretical ground for improving the survival rate of bottom-sowing, propagation, and release of S. subcrenata seedlings.

show abstract

The participation of electrolytes in adaptation mechanisms of intertidal molluscs' cells to altered salinity

Cited by 22 publications

References 2 publications

Calcification in a marginal sea – influence of seawater [Ca<sup>2+</sup>] and carbonate chemistry on bivalve shell formation

Calcification in a marginal sea – influence of seawater [Ca<sup>2+</sup>] and carbonate chemistry on bivalve shell formation

Resistance to freshwater exposure in White Sea Littorina spp. I: Anaerobic metabolism and energetics

Effects of Sudden Drop in Salinity on Osmotic Pressure Regulation and Antioxidant Defense Mechanism of Scapharca subcrenata

Contact Info

Product

Resources

About

The participation of electrolytes in adaptation mechanisms of intertidal molluscs' cells to altered salinity

Cited by 22 publications

References 2 publications

Calcification in a marginal sea – influence of seawater [Ca&lt;sup&gt;2+&lt;/sup&gt;] and carbonate chemistry on bivalve shell formation

Calcification in a marginal sea – influence of seawater [Ca&lt;sup&gt;2+&lt;/sup&gt;] and carbonate chemistry on bivalve shell formation

Resistance to freshwater exposure in White Sea Littorina spp. I: Anaerobic metabolism and energetics

Effects of Sudden Drop in Salinity on Osmotic Pressure Regulation and Antioxidant Defense Mechanism of Scapharca subcrenata

Contact Info

Product

Resources

About

Calcification in a marginal sea – influence of seawater [Ca<sup>2+</sup>] and carbonate chemistry on bivalve shell formation

Calcification in a marginal sea – influence of seawater [Ca<sup>2+</sup>] and carbonate chemistry on bivalve shell formation