The Pathogenesis of Superficial Fungous Infections in Cultured Human Skin

Blank, Harvey

doi:10.1001/archderm.1959.01560170022005

Cited by 67 publications

(16 citation statements)

References 25 publications

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“…It has been suggested that invasion is limited by local factors within the deeper nucleated epithelial cells which are not conducive to fungal growth (Taschdjian et al, 1960). While such local factors may have been lost in vitro it would seem more likely that the deep penetration reflects the absence of systemic components within the culture system, as suggested by Blank et al (1959). It is interesting to note that an intercellular permeability barrier has been demonstrated in the superficial layers of the epithelium of both skin (Schreiner and Wolff, 1969) and oral mucosa (Squier, 1973).…”

Section: Discussionmentioning

confidence: 98%

The infection of rat tongue mucosa in vitro with five species of candida

Howlett

1976

Journal of Medical Microbiology

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Section: Discussionmentioning

confidence: 98%

The infection of rat tongue mucosa in vitro with five species of candida

Howlett

1976

Journal of Medical Microbiology

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“…1957 (12); Lasnitzuki, 1958 (13)). Blank et al (1959) (14) cultured foreskin of newborn infant and adult human skin with a lens paper and agar,method, and found mitotic figures in the epidermis after 4 weeks cultivation in tntro. Reinertson (1961) (15) described a successful cultivation of explants of adult human skin in cellulose sponge for a period of up to 10 days.…”

Section: Discussionmentioning

confidence: 99%

Organ Culture of Adult Human Skin

Yasuno

Maeda

Sato

et al. 1980

The Journal of Dermatology

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Proliferation and differentiation of the epidermis in organ culture of adult human skin by the sponge matrix method were studied histologically and autoradiographically, and the following results were obtained: 1) On the first day of culture, mitotic figures were already observable in the epidermis. The outgrowth of epidermal cells at the margins of the explants started. On the second day, there was transformation to a zone that will be referred to as the newly formed stratum corneum in the upper epidermis. 2) On the third and fourth days, the increased growth of epidermal cells caused thickening of the epidermis. Simultaneously, Malpighian cells progressively differentiated into a cornified layer. 3) On and after the fifth day, the basospinous cell layer was reduced in thickness in most of explants. On the ninth and tenth days, DNA synthesis in the basal layer was still obvious, although the epidermis showed a thickness of only one or two cells overlaid with a large number of horny layers. 4) In the culture medium supplemented with corticosteroid, the epidermal growth was slightly depressed with lessened formation of stratum corneum in the early stages of culture as compared with the explants cultured in the basic medium. The reduction of the basospinous layer was scarcely notable after the fifth day. Even after 10-11 days, epidermal cells were well preserved and their stratified squamous architecture was less disorganized. It seemed that corticosteroid could prolong the survival of adult human skin in vitro. These findings indicate that this culture technique could be used as a model for organ culture of adult human skin.

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“…Normal human serum has exhibited fungistatic activity toward the growth of dermatophytes (Peck et al, 1940;Lorincz et al, 1958;Blank et al, 1959;. The inhibitory action, expressed in vitro, was reduced by dialysis and by heat for 30 min at 56°C, and therefore was not an immunoglobulin.…”

Section: Serum Factorsmentioning

confidence: 99%

Immunopathology

1983

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The Pathogenesis of Superficial Fungous Infections in Cultured Human Skin

Cited by 67 publications

References 25 publications

The infection of rat tongue mucosa in vitro with five species of candida

The infection of rat tongue mucosa in vitro with five species of candida

Organ Culture of Adult Human Skin

Immunopathology

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