The pathogenic potential of &lt;i&gt;Helicobacter cinaedi&lt;/i&gt; isolated from non-human sources: adherence, invasion and translocation ability in polarized intestinal epithelial Caco-2 cells &lt;i&gt;in vitro&lt;/i&gt;

Taniguchi, Takako; Yamazaki, Wataru; Saeki, Yûji; Takajo, Ichiro; Okayama, Akira; Hayashi, Tetsuya; Misawa, Naoaki

doi:10.1292/jvms.15-0595

Cited by 14 publications

(22 citation statements)

References 29 publications

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“…indicated that community‐acquired infections are also possible. Finally, it has been hypothesized that animals can be a source of infection . It was shown that H. cinaedi isolates from dogs and hamsters were able to adhere and invade human intestinal epithelial Caco‐2 cells in vitro …”

Section: Helicobacter Cinaedi: An Emerging Pathogen In Human Patientsmentioning

confidence: 99%

“…Finally, it has been hypothesized that animals can be a source of infection. 47,55,59 It was shown that H. cinaedi isolates from dogs and hamsters were able to adhere and invade human intestinal epithelial Caco-2 cells in vitro. 59 Up till now, no susceptibility testing guidelines or standard therapy is available.…”

Section: Helicobacter Cinaedi: An Emerging Pathogen In Human Patientsmentioning

confidence: 99%

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Other Helicobacters and gastric microbiota

et al. 2016

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This article aimed to review the literature from 2015 dealing with gastric and enterohepatic non-Helicobacter pylori Helicobacter species (NHPH). A summary of the gastric microbiota interactions with H. pylori is also presented. An extensive number of studies were published during the last year and have led to a better understanding of the pathogenesis of infections with NHPH. These infections are increasingly reported in human patients, including infections with H. cinaedi, mainly characterized by severe bacteremia. Whole-genome sequencing appears to be the most reliable technique for identification of NHPH at species level. Presence of NHPH in laboratory animals may influence the outcome of experiments, making screening and eradication desirable. Vaccination based on UreB proteins or bacterial lysate with CCR4 antagonists as well as oral glutathione supplementation may be promising strategies to dampen the pathogenic effects associated with gastric NHPH infections. Several virulent factors such as outer membrane proteins, phospholipase C-gamma 2, Bak protein, and nickel-binding proteins are associated with colonization of the gastric mucosae and development of gastritis. The development of high-throughput sequencing has led to new insights in the gastric microbiota composition and its interaction with H. pylori. Alterations in the gastric microbiota caused by the pH-increasing effect of a H. pylori infection may increase the risk for gastric cancer.

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Section: Helicobacter Cinaedi: An Emerging Pathogen In Human Patientsmentioning

confidence: 99%

Section: Helicobacter Cinaedi: An Emerging Pathogen In Human Patientsmentioning

confidence: 99%

Other Helicobacters and gastric microbiota

et al. 2016

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“…H. pullorum has been related to diarrhea, gastroenteritis and liver disease in humans (Ceelen et al, 2005). Also, H. cinaedi causes bacteremia, cellulitis, septicemia and enteritis in immunocompromised, immunocompetent and healthy persons (Taniguchi et al, 2016).…”

Section: Resultsmentioning

confidence: 99%

Helicobacter pylori in ice cream and its control using mastic gum essential oil

Saad¹,

El‐Zamkan²

2017

J Adv Vet Anim Res

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Objective: This study aimed at assessing the prevalence of Helicobacter spp. in ice cream sold at Qena city (Egypt) with special reference to H. pylori, and to study the antimicrobial effect of mastic gum essential oil on H. pylori. Materials and methods: A total of 60 small and large scale ice cream samples (30 each) were investigated for the presence of Helicobacter spp. Then, the essential oil of mastic gum was added to lab prepared ice cream inoculated with H. pylori isolate in a count of Log6 using 2 concentrations of 0.8 and 1.6% and one group was kept as a control group. Each group was subdivided into 2 sub-groups; one was kept at -5°C and another one was kept at -20°C. Results: Helicobacter spp. could be isolated from 11.7%, while, H. pylori could be found in 3.3% of the total examined ice cream samples. Regarding the antiHelicobacter pylori effect of mastic gum essential oil added to lab prepared ice cream, in samples kept at -5°C it was found that after hardening the count fall into Log5 in both control and 0.8% groups and log4 in 1.6% essential mastic oil concentration. Reduction in H. pylori at the first week and third day of storage reached 100% where H. pylori couldn't be counted or isolated from both 0.8 and 1.6% concentrations, respectively. While in ice cream samples stored at -20°C, the reduction in H. pylori load was rapid at first, it was log4 and log3 in groups of 0.8% and 1.6% concentrations, respectively versus log5 in control samples. At the third day H. pylori was not able to be counted or isolated at third day of storage for both concentrations. Conclusion: All isolates recovered from small scale ice cream samples reflexing the hygienic conditions under which samples were produced. Mastic gum essential oil exhibited a powerful anti-H. pylori effect recommending its addition to food matrix for therapeutic purposes or as a functional food.

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“…It is certainly possible that the growth-promoting effect of epithelial cells is attributable to multiple factors, as the relationship between H. cinaedi and epithelial cells is potentially complex. This is underscored by recently published data on the physical interactions between the organism and polarized Caco-2 monolayers (60). Although that study did not address the phenomenon of induced aerobic growth, it reported the ability of H. cinaedi to adhere to, invade, and translocate across the cells.…”

Section: Discussionmentioning

confidence: 96%

Epithelial Coculture and l -Lactate Promote Growth of Helicobacter cinaedi under H ₂ -Free Aerobic Conditions

Schmitz

Taniguchi

Misawa

et al. 2016

Appl Environ Microbiol

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Helicobacter cinaedi is an emerging opportunistic pathogen associated with infections of diverse anatomic sites. Nevertheless, the species demonstrates fastidious axenic growth; it has been described as requiring a microaerobic atmosphere, along with a strong preference for supplemental H 2 gas. In this context, we examined the hypothesis that in vitro growth of H. cinaedi could be enhanced by coculture with human epithelial cells. When inoculated (in Ham's F12 medium) over Caco-2 monolayers, the type strain (ATCC BAA-847) gained the ability to proliferate under H 2 -free aerobic conditions. Identical results were observed during coculture with several other monolayer types (LS-174T, AGS, and HeLa). Under chemically defined conditions, 40 amino acids and carboxylates were screened for their effect on the organism's atmospheric requirements. Several molecules promoted Helicobacter cinaedi is an emerging pathogen of the class Epsilonproteobacteria. This spiral-shaped Gram-negative rod is associated with opportunistic infections of multiple anatomic sites, including the intestinal tract (1, 2), blood (3, 4), peripheral soft tissue (5, 6), and other body fluids (7-9). This behavior contrasts with related Helicobacter and Campylobacter species, which typically demonstrate a more limited tissue tropism. H. cinaedi was identified ϳ30 years ago in men who have sex with men (1) and HIV patients (10, 11), although infections have since been described in other immunocompromised groups (12, 13), as well as immunocompetent (14-17) and postsurgical patients (18).Despite abundant case reports, the ecology/epidemiology of H. cinaedi remains poorly defined and sparsely studied. Limited molecular evidence suggests that it can be found in the stools of a small percentage of healthy individuals (19). The organism has also been isolated from animal feces (including rodents, dogs, and primates), although its role as a veterinary and/or zoonotic pathogen is uncertain (20,21). An environmental reservoir has not yet been identified. The majority of recent literature on H. cinaedi (for instance, many of the current references) originates from Japan, although it is unclear whether this reflects regional prevalence or an increased awareness among Japanese investigators. Knowledge of H. cinaedi biology and tools for its genetic manipulation are likewise sparse, limited to several studies of targeted deletion mutants. These deletion mutants include strains deficient in cytolethal distending toxin (22) and alkyl hydroperoxide reductase (23), both of which demonstrate attenuated virulence in a murine colitis model.Likely contributing to this paucity of information, H. cinaedi is a challenging organism to manipulate in vitro. Strains are notable for poor, film-like growth on agar plates, a preference for biphasic culture (broth over agar), and decreased viability on subculture (24, 25). Recommended medium conditions for H. cinaedi are not

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The pathogenic potential of <i>Helicobacter cinaedi</i> isolated from non-human sources: adherence, invasion and translocation ability in polarized intestinal epithelial Caco-2 cells <i>in vitro</i>

Cited by 14 publications

References 29 publications

Other Helicobacters and gastric microbiota

Other Helicobacters and gastric microbiota

Helicobacter pylori in ice cream and its control using mastic gum essential oil

Epithelial Coculture and l -Lactate Promote Growth of Helicobacter cinaedi under H ₂ -Free Aerobic Conditions

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The pathogenic potential of <i>Helicobacter cinaedi</i> isolated from non-human sources: adherence, invasion and translocation ability in polarized intestinal epithelial Caco-2 cells <i>in vitro</i>

Cited by 14 publications

References 29 publications

Other Helicobacters and gastric microbiota

Other Helicobacters and gastric microbiota

Helicobacter pylori in ice cream and its control using mastic gum essential oil

Epithelial Coculture and l -Lactate Promote Growth of Helicobacter cinaedi under H 2 -Free Aerobic Conditions

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Product

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Epithelial Coculture and l -Lactate Promote Growth of Helicobacter cinaedi under H ₂ -Free Aerobic Conditions