The Pathway of Dephosphorylation of<i>myo</i>-Inositol Hexakisphosphate by Phytases from Wheat Bran of<i>Triticum aestivum</i>L. cv. Nourin #61

Nakano, Tadao; Joh, Toshio; Narita, Kazumasa; Hayakawa, Tetsuo

doi:10.1271/bbb.64.995

Cited by 45 publications

(33 citation statements)

References 15 publications

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“…As shown, there is one major and two minor degradation pathways for wheat phytase: the major pathway proceeds via d-I(1,2,3,5,6)P 5 , d-I(1,2,3,6)P 4 , and d-I(1,2,5,6)P 4 , I(1,2,3)P 3 -d-I(1,2,6)P 3 , d/l-I(1,2)P 2 , and finally into I(2)P 1 and d/l-I(1)P 1 . This pathway of degradation is consistent with published results (Chen and Li, 2003;Nakano et al, 2000). One minor route proceeds via d/l-I(1,2,4,5,6)P 5, d/l-I(1,2,5,6)P 4 , d/l-I(1,2,6)P 3 , d/l-I(1,2)P 2 , I(2)P 1 , and then to d/l-I(1)P 1 , similar to our previous study (Wu et al, 2015).…”

Section: Major and Minor Pathways Of Phytate Degradationsupporting

confidence: 93%

“…For wheat phytase, for example, d/l-I(1,2,3,4,5)P 5 [equivalent to d/l-I(1,2,3,5,6)P 5 ] was detected as the major IP 5 isomer at the initial stage of degradation. Because wheat phytase is a 6-phytase, d/l-I(1,2,3,4,5)P 5 should be d-I(1,2,3,5,6)P 5 with a phosphate group released from the d-4 position (Lim and Tate, 1973;Nakano et al, 2000). This also narrows down the possibilities of further dephosphorylation products from d-I(1,2,3,5,6)P 5 .…”

Section: Major and Minor Pathways Of Phytate Degradationmentioning

confidence: 99%

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Phytate Degradation by Different Phosphohydrolase Enzymes: Contrasting Kinetics, Decay Rates, Pathways, and Isotope Effects

Sun

Alikhani

Massoudieh

et al. 2017

Soil Science Soc of Amer J

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Phytate (IP 6 ) is often the most common organic P compound particularly in agricultural soils. understanding the fate of inositol phosphate (IP x ) in the environment in terms of isomeric composition and concentration and assessing relative resistance to (or preference for) degradation is essential to estimate the potential role of IP x in generating inorganic P (P i ) as well as overall P cycling in the environment. In this study, we analyzed IP 6 degradation by four common phosphohydrolase enzymes (phytase from wheat [Triticum aestivum l.] and Aspergillus niger and acid phosphatase from wheat germ and potato [Solanum tuberosum l.]), with particular focus on degradation pathways, isomer kinetic decay rate, and isotope effects using a combination of high-performance ion chromatography, nuclear magnetic resonance, stable isotopes, and process-based modeling techniques. our results show that the degradation pathways are often distinct among enzymes. The process-based Bayesian inverse modeling was used to capture the trend and magnitude of the measured concentrations for each IP x isomer and to determine the decay constants. Furthermore, o isotope ratios (d 18 o P ) of released P i enabled the identification of isotopically identical phosphate moieties in phytate derived from natural sources. Distinctly different fractionation factors, degradation pathways, and kinetic decay rate coefficients among the enzymes studied could lead to potential discrimination and tracking of phytate sources and products as well as active enzymes present in the environment.Abbreviations: HPIC, high-performance ion chromatography; IP, inositol phosphate; NMR, nuclear magnetic resonance; P i , inorganic phosphorus; pNPP, para-nitrophenyl phosphate.I nositol phosphates are a group of organic P compounds widely present in the natural environment (Turner et al., 2002). Phytate (the salt of myo-inositol 1,2,3,4,5,6-hexakisphosphate or IP 6 ) is a P storage molecule in cereals and grains and represents between 60 and 80% of P in mature seeds (Raboy, 1997). Since it is reported that ?51 million tonnes of phytate is formed in commercially produced fruits and crop seeds every year (Lott et al., 2000), a good fraction of phytate in seeds and grains is released to the soil environment as plant residues and animal manures (Dao, 2007;Gerke, 2015). Phytate readily sorbs onto minerals or precipitates with soil cations and organic matter, and then accumulates to constitute an often dominant class of organic P (Celi and Barberis, 2007;Giles and Cade-Menun, 2014). Although sorption and precipitation immobilizes a large fraction of phytate in soil, there is a potential for its transfer to water bodies with soil particulates and colloids (Turner et al., 2002;Turner and Newman, 2005 Core Ideas• Phytate is degraded through distinct pathways for a particular enzyme.• oxygen isotope ratios of phosphate moieties in phytate are isotopically identical.• These findings bring new insights into tracking phytate sources in the environment.

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Section: Major and Minor Pathways Of Phytate Degradationsupporting

confidence: 93%

Section: Major and Minor Pathways Of Phytate Degradationmentioning

confidence: 99%

Phytate Degradation by Different Phosphohydrolase Enzymes: Contrasting Kinetics, Decay Rates, Pathways, and Isotope Effects

Sun

Alikhani

Massoudieh

et al. 2017

Soil Science Soc of Amer J

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“…In vitro, the InsPs that were primarily formed by phytases purified from wheat constituted Ins(1,2,3,4,5)P 5 , Ins(1,2,5,6)P 4 and Ins(1,2,6)P 3 (Nakano et al 2000), whereas Ins (1,2,4,5,6)P 5 , Ins(1,2,3,4)P 4 and Ins(1,2,3)P 3 were formed to a lesser extent (Nakano et al 2000;Bohn et al 2007). In vitro, the InsPs that were primarily formed by phytases purified from wheat constituted Ins(1,2,3,4,5)P 5 , Ins(1,2,5,6)P 4 and Ins(1,2,6)P 3 (Nakano et al 2000), whereas Ins (1,2,4,5,6)P 5 , Ins(1,2,3,4)P 4 and Ins(1,2,3)P 3 were formed to a lesser extent (Nakano et al 2000;Bohn et al 2007).…”

Section: Insps In the Presence Of Low And High Intrinsic Phytase Actimentioning

confidence: 99%

Dietary effects on inositol phosphate breakdown in the crop of broilers

Zeller

Schollenberger

Kühn

et al. 2015

Archives of Animal Nutrition

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The effect of diets differing in enzyme supplements, mineral phosphorus (P) and microwave wheat treatment on phytate hydrolysis and lower inositol phosphate isomers (InsPs) appearance in broiler crops was studied. The broilers (16- and 15-day-old) were assigned to 48 pens of 15 or 20 birds each (n = 8 pens per treatment) in Experiments 1 and 2, respectively. In Experiment 1, birds received a low-P wheat-soybean meal diet where the wheat was either microwave treated or not. These diets were offered without further supplementation or with added phytase (500 FTU/kg diet), alone or in combination with a xylanase (16,000 BXU/kg diet). In Experiment 2, two maize-soybean meal-based diets were fed, without or with monocalcium phosphate supplementation. Furthermore, these diets were offered without further supplementation or with phytase at 500 or 12,500 FTU/kg diet. On day 23 or 24 (Experiments 1 and 2, respectively), crop digesta were pooled per pen, freeze-dried and analysed for InsPs and the marker TiO2. Microwaving reduced the intrinsic phytase activity and InsP6 hydrolysis, but increased the concentration of Ins(1,2,3,4,5)P5 and Ins(1,2,4,5,6)P5 in the digesta of crop (Experiment 1). Microwave treatment significantly interacted with enzyme supplementation for Ins(1,2,5,6)P4 concentration, indicating a synergistic effect of intrinsic and supplied phytase in the crop. Xylanase tended to support phytase hydrolysis in diets with microwave-treated wheat. Phytase addition increased InsP6 hydrolysis up to 79% (Experiment 2). Thus, wheat phytase activity can cause high InsP6 hydrolysis in the crop. Treatment differences in lower InsPs indicated that hydrolysis of the first InsP6 phosphate group is not the only step in the degradation cascade in the crop of broilers that is influenced by dietary factors.

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“…Because different structural isomers may result in different physiological functions, identification of the myo-inositol phosphates generated during enzymatic phytate degradation is of great importance to exploit the full potential of the naturally occurring phytases. The complete phytate degradation pathway was elucidated for only a few phytases, focusing on those of microbial (1- 3,11,22) or cereal origin (4)(5)(6)(7). There is only one paper describing the phytate degradation pathway of a legume phytase (8).…”

Section: Introductionmentioning

confidence: 99%

Pathway of Dephosphorylation of myo-Inositol Hexakisphosphate by Phytases of Legume Seeds

Greiner

Alminger

Carlsson

et al. 2002

J. Agric. Food Chem.

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Using a combination of high-performance ion chromatography analysis and kinetic studies, the pathway of dephosphorylation of myo-inositol hexakisphosphate by the phytases purified from faba bean and lupine seeds, respectively, was established. The data demonstrate that the legume seed phytases under investigation dephosphorylate myo-inositol hexakisphosphate in a stereospecific way. The phytase from faba bean seeds and the phytase LP2 from lupine seeds degrade phytate by sequential removal of phosphate groups via D-Ins(1,2,3,5,6)P(5), D-Ins(1,2,5,6)P(4), D-Ins(1,2,6)P(3), and D-Ins(1,2)P(2) to finally Ins(2)P, whereas the phytases LP11 and LP12 from lupine seeds generate the final degradation product Ins(2)P via D-Ins(1,2,4,5,6)P(5), D-Ins(1,2,5,6)P(4), D-Ins(1,2,6)P(3), and D-Ins(1,2)P(2).

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The Pathway of Dephosphorylation ofmyo-Inositol Hexakisphosphate by Phytases from Wheat Bran ofTriticum aestivumL. cv. Nourin #61

Cited by 45 publications

References 15 publications

Phytate Degradation by Different Phosphohydrolase Enzymes: Contrasting Kinetics, Decay Rates, Pathways, and Isotope Effects

Phytate Degradation by Different Phosphohydrolase Enzymes: Contrasting Kinetics, Decay Rates, Pathways, and Isotope Effects

Dietary effects on inositol phosphate breakdown in the crop of broilers

Pathway of Dephosphorylation of myo-Inositol Hexakisphosphate by Phytases of Legume Seeds

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