The pattern of major polymorphisms in the Duffy binding protein ligand domain among Plasmodium vivax isolates from the Brazilian Amazon area

Sousa, Taís Nóbrega de; Cerávolo, Isabela P.; Fontes, Cor Jésus Fernandes; Couto, Álvaro Augusto Ribeiro D'Almeida; Carvalho, Luzia H.; Brito, Cristiana Ferreira Alves de

doi:10.1016/j.molbiopara.2005.11.006

Cited by 34 publications

(38 citation statements)

References 16 publications

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“…A high frequency (> 50%) of D384G (85.2%), R390H (63.0%), L424I (83.3%), W437R (61.1%), and I503K (77.7%) residues were found in Myanmar isolates compared to that in the Sal I sequence. This was highly similar to a report on Thailand isolates (D384G, 76.7%; R390H, 56.7%; L424I, 86.7%; W437R, 63.3%; I503K, 56.7%) [20] but differed from previous studies showing R308S (67%), D384G (66%), and S447K (59%) in Papua New Guinean isolates; D384G (59%) in Colombian isolates; D384G (85%) and I503K (55%) in Brazilian isolates; D384G (61.3%) and I503K (70.6%) in Iranian isolates; and D384G (94%) and I503K (55%) in Sri Lankan isolates [19,21,31]. F306L, which has only been reported from Asian malaria endemic areas, including Thailand [20], Iran [21], and Sri Lanka [31], was also identified in the Myanmar isolates.…”

Section: Discussioncontrasting

confidence: 68%

“…Phylogenetic analysis revealed that the 12 Myanmar haplotypes were widely distributed among different isolates from distinct geographic regions (Figure 2). Comparison of the most common variants in PvDBPII among presently studied P. vivax populations revealed that Myanmar isolates showed a pattern similar to Thailand isolates [20] but one that differed from Papua New Guinean, Colombian, Brazilian, Iranian, and Sri Lankan isolates [19,21,31] (Table 1). Although the Myanmar isolates showed similar amino acid changes compared to Thailand isolates, nine variants found in the Thailand isolates (R268S, S351C, I367T, S398T, T404R, Q433K, R436T, N507H, and T513K) were not identified in the Myanmar isolates.…”

Section: Resultsmentioning

confidence: 99%

“… a [20]; b [21]; c [31]; d [19]The first letter represents the amino acid in that position in Sal I sequence, and the other letter represents the substituted amino acid…”

Section: Resultsmentioning

confidence: 99%

“…Some polymorphic residues in PvDBPII occurred in only one population or geographic region, but common variant amino acids (K371E, D384G, E385K, K386N, N417K, L424I, W437R and I503K) are found in global isolates [19-21,31]. A high frequency (> 50%) of D384G (85.2%), R390H (63.0%), L424I (83.3%), W437R (61.1%), and I503K (77.7%) residues were found in Myanmar isolates compared to that in the Sal I sequence.…”

Section: Discussionmentioning

confidence: 99%

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Genetic polymorphism and natural selection of Duffy binding protein of Plasmodium vivax Myanmar isolates

Kang

Moon

Kim

et al. 2012

Malar J

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BackgroundPlasmodium vivax Duffy binding protein (PvDBP) plays an essential role in erythrocyte invasion and a potential asexual blood stage vaccine candidate antigen against P. vivax. The polymorphic nature of PvDBP, particularly amino terminal cysteine-rich region (PvDBPII), represents a major impediment to the successful design of a protective vaccine against vivax malaria. In this study, the genetic polymorphism and natural selection at PvDBPII among Myanmar P. vivax isolates were analysed.MethodsFifty-four P. vivax infected blood samples collected from patients in Myanmar were used. The region flanking PvDBPII was amplified by PCR, cloned into Escherichia coli, and sequenced. The polymorphic characters and natural selection of the region were analysed using the DnaSP and MEGA4 programs.ResultsThirty-two point mutations (28 non-synonymous and four synonymous mutations) were identified in PvDBPII among the Myanmar P. vivax isolates. Sequence analyses revealed that 12 different PvDBPII haplotypes were identified in Myanmar P. vivax isolates and that the region has evolved under positive natural selection. High selective pressure preferentially acted on regions identified as B- and T-cell epitopes of PvDBPII. Recombination may also be played a role in the resulting genetic diversity of PvDBPII.ConclusionsPvDBPII of Myanmar P. vivax isolates displays a high level of genetic polymorphism and is under selective pressure. Myanmar P. vivax isolates share distinct types of PvDBPII alleles that are different from those of other geographical areas. These results will be useful for understanding the nature of the P. vivax population in Myanmar and for development of PvDBPII-based vaccine.

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Section: Discussioncontrasting

confidence: 68%

Section: Resultsmentioning

confidence: 99%

“… a [20]; b [21]; c [31]; d [19]The first letter represents the amino acid in that position in Sal I sequence, and the other letter represents the substituted amino acid…”

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Genetic polymorphism and natural selection of Duffy binding protein of Plasmodium vivax Myanmar isolates

Kang

Moon

Kim

et al. 2012

Malar J

View full text Add to dashboard Cite

show abstract

“…Individuals that lack the Duffy surface antigen on their erythrocytes are naturally resistant to P. vivax malaria. Thus, P. vivax DBP provides an attractive target for vaccine-mediated immunity (Xainli et al 2000, Souza et al 2006.…”

Section: Duffy Binding Proteinmentioning

confidence: 99%