The pea (Pisum sativum L.) genes sym33 and sym40 control infection thread formation and root nodule function

Tsyganov, V. E.; Morzhina, E. V.; Stefanov, S. Y.; Borisov, A. Y.; Lebsky, V. K.; Тихонович, И. А.

doi:10.1007/s004380050840

Cited by 87 publications

(107 citation statements)

References 37 publications

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“…Besides sym31, many other legume Fix 2 mutants have related symbiosome defects as observed here (Tsyganov et al, 1998;Borisov et al, 1992;Morzhina et al, 2000;Suganuma et al, 2003). However, the phenotype of most of these mutants does not fully correspond to that of 35S:SINAT5DN.…”

Section: Mtsina Proteins Are Involved In the Formation Of Its And In mentioning

confidence: 63%

“…However, the phenotype of most of these mutants does not fully correspond to that of 35S:SINAT5DN. To our knowledge, the most similar mutant is sym33/SGRfix-2 that makes fewer nodules with thick ITs, which are often closed and in which bacteroids do not develop properly after sporadic release into the plant cells (Tsyganov et al, 1998). Unfortunately, the interrupted gene is still unknown.…”

Section: Mtsina Proteins Are Involved In the Formation Of Its And In mentioning

confidence: 99%

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Seven in Absentia Proteins Affect Plant Growth and Nodulation inMedicago truncatula

et al. 2008

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Protein ubiquitination is a posttranslational regulatory process essential for plant growth and interaction with the environment. E3 ligases, to which the seven in absentia (SINA) proteins belong, determine the specificity by selecting the target proteins for ubiquitination. SINA proteins are found in animals as well as in plants, and a small gene family with highly related members has been identified in the genome of rice (Oryza sativa), Arabidopsis (Arabidopsis thaliana), Medicago truncatula, and poplar (Populus trichocarpa). To acquire insight into the function of SINA proteins in nodulation, a dominant negative form of the Arabidopsis SINAT5 was ectopically expressed in the model legume M. truncatula. After rhizobial inoculation of the 35S:SINAT5DN transgenic plants, fewer nodules were formed than in control plants, and most nodules remained small and white, a sign of impaired symbiosis. Defects in rhizobial infection and symbiosome formation were observed by extensive microscopic analysis. Besides the nodulation phenotype, transgenic plants were affected in shoot growth, leaf size, and lateral root number. This work illustrates a function for SINA E3 ligases in a broad spectrum of plant developmental processes, including nodulation.

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Section: Mtsina Proteins Are Involved In the Formation Of Its And In mentioning

confidence: 63%

Section: Mtsina Proteins Are Involved In the Formation Of Its And In mentioning

confidence: 99%

Seven in Absentia Proteins Affect Plant Growth and Nodulation inMedicago truncatula

et al. 2008

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“…Ранее по гену sym33 были описаны 2 мутации: у му-танта SGEFix --2 (Tsyganov et al, 1998), индуцированная c использованием линии SGE, а также у мутанта RisFixU, полученная на сорте Finale (Engvild, 1987;Borisov et al, 2004). Для гена sym40 ранее была известна лишь одна уникальная мутация у мутанта SGEFix --1 (Tsyganov et al, 1998), полученная на основе линии SGE. Для гена sym27 известны 2 мутации: у мутанта p12, индуцирован-ная на сорте Frisson (Duc, Messager, 1989;Borisov et al, 2004), и у мутанта RisFixQ, полученная на основе сорта Finale (Engvild, 1987;Borisov et al, 2004).…”

Section: гибридологический анализunclassified

Novel series os pea symbiotic mutants induced in the SGE line

Tsyganov

Voroshilova²,

Розов

et al. 2012

Ecological genetics

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Using ethylmethansulphonate the chemical mutagenesis of the pea laboratory line SGE was performed. During analysis of 425 families (2069 plants) of М2 progeny 45 putative mutants were selected, among them 30 mutants forming ineffective nodules (Fix– phenotype), 13 mutantsunable to form nodules (Nod– phenotype), and 2 mutants forming a few nodules (Nod+/– phenotype). For 1 Nod– and 5 Fix– mutants monogenic inheritance and recessive phenotype manifestation were demonstrated. For Fix– mutant SGEFix––9 an additional mutation leading to Nod+/– phenotype was shown. Complementation analysis showed that the mutant phenotype of the SGEFix- - 5 line is caused by a mutation in the sym33 gene, of theSGEFix––6 linein the sym40 gene, of the SGEFix––7 line in the sym27 gene, and of the SGEFix––8 linein the sym25 gene.

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“…В исследовании были использованы растения исход-ной линии гороха посевного (Pisum sativum L.) SGE (Kosterin, Rozov, 1993) и неэффективный (неспособ-ный к эффективной азотфиксации) мутант SGEFix¯-1 (sym40), характеризующийся гипертрофированным развитием инфекционных капель и ранним старением (Tsyganov et al, 1998).…”

Section: растительный материалunclassified

“…В настоящем исследовании как у исходной линии SGE, так и мутанта SGEFix¯-1 (sym40) наблюдалась характер-ная для этих линий гистологическая и ультраструктурная организация клубеньков (Tsyganov et al, 1998).…”

Section: обсуждение результатовunclassified

Comparative cytochemical analysis of hydrogen peroxide distribution in pea ineffective mutant SGEFix--1 (sym40) and initial line SGE

Tsyganova

Tsyganov

Borisov

et al. 2009

Ecological genetics

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Comparative cytochemical analysis has revealed differences in hydrogen peroxide distribution in symbiotic nodules of pea initial line SGE and mutant SGEFix--1 (sym40). In the initial line SGE, precipitates of cerium perhydroxide were deposited in the walls of infection threads and in adjacent material in the luminal matrix. In mutant SGEFix--1, an increased deposition of cerium perhydroxide precipitates was observed in the matrix of hypertrophied infection droplets, round bacteria contained in infection threads and also around juvenile bacteroids. The observed pattern of hydrogen peroxide distribution indicates that bacteria in infected cells of mutant nodules are exposed to a stronger oxidative stress compared with nodules of the initial line.

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The pea (Pisum sativum L.) genes sym33 and sym40 control infection thread formation and root nodule function

Cited by 87 publications

References 37 publications

Seven in Absentia Proteins Affect Plant Growth and Nodulation inMedicago truncatula

Seven in Absentia Proteins Affect Plant Growth and Nodulation inMedicago truncatula

Novel series os pea symbiotic mutants induced in the SGE line

Comparative cytochemical analysis of hydrogen peroxide distribution in pea ineffective mutant SGEFix--1 (sym40) and initial line SGE

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