The PEG-responding desiccome of the alder microsymbiont Frankia alni

Ghedira, Kaïs; Harigua‐Souiai, Emna; Hamda, Cherif Ben; Fournier, Pascale; Pujic, Petar; Guesmi, Sihem; Guizani, Ikram; Miotello, Guylaine; Armengaud, Jean; Normand, Philippe; Sghaier, Haïtham

doi:10.1038/s41598-017-18839-0

Cited by 16 publications

(14 citation statements)

References 85 publications

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“…Its proximity on some genomes of cluster 1 strains to the hybrid lectin‐kinase (FRAAL5298) is evocative of a lineage‐specific response to host polysaccharides (Pujic et al, ). A recent analysis of the response of Frankia alni cells to a PEG osmotic shock (Ghedira et al, ) has yielded a large number of upregulated proteins but few were found in the present study, suggesting the PEG response and the response to exudates are contrasted.…”

Section: Discussioncontrasting

confidence: 60%

Omics of the early molecular dialogue between Frankia alni and Alnus glutinosa and the cellulase synton

Pujic

Alloisio

Fournier

et al. 2019

Environmental Microbiology

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Summary The early Frankia‐Alnus symbiotic molecular exchanges were analyzed in detail by protein and RNA omics. For this, Frankia cells were placed in the presence of Alnus roots but separated by a dialysis membrane for 64 h. The bacterial cells were then harvested and analyzed by high‐throughput proteomics and transcriptomics (RNA‐seq). The most upregulated gene clusters were found to be the potassium transporter operon kdp and an ABC transporter operon of uncharacterized function. The most upregulated proteins were found to be acyl dehydrogenases and the potassium transporter Kdp. These suggest a preadaptation to the impending stresses linked to the penetration into isotonic host tissues and a possible rearrangement of the membrane. Another cluster among the 60 most upregulated ones that comprised two cellulases and a cellulose synthase was conserved among the Frankia and other actinobacteria such as Streptomyces. Cellulase activity was detected on CMC all along the length of the root but not away from it. Frankia alni ACN14a was found to be unable to respire or grow on glucose as sole carbon source. The cellulose synthase was found active at the tip of hyphae in response to Alnus root exudates, resulting in a calcofluor stained tip.

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Section: Discussioncontrasting

confidence: 60%

Omics of the early molecular dialogue between Frankia alni and Alnus glutinosa and the cellulase synton

Pujic

Alloisio

Fournier

et al. 2019

Environmental Microbiology

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“…Other in vitro proteomic studies have been done of Frankia with/without ammonium [ 22 ] or under osmotic stress [ 23 ] and have shown TCA involvement, stress determinants and various regulators.…”

Section: Introductionmentioning

confidence: 99%

The Proteogenome of Symbiotic Frankia alni in Alnus glutinosa Nodules

et al. 2022

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Omics are the most promising approaches to investigate microbes for which no genetic tools exist such as the nitrogen-fixing symbiotic Frankia. A proteogenomic analysis of symbiotic Frankia alni was done by comparing those proteins more and less abundant in Alnus glutinosa nodules relative to N2-fixing pure cultures with propionate as the carbon source. There were 250 proteins that were significantly overabundant in nodules at a fold change (FC) ≥ 2 threshold, and 1429 with the same characteristics in in vitro nitrogen-fixing pure culture. Nitrogenase, SuF (Fe–Su biogenesis) and hopanoid lipids synthesis determinants were the most overabundant proteins in symbiosis. Nitrogenase was found to constitute 3% of all Frankia proteins in nodules. Sod (superoxide dismutase) was overabundant, indicating a continued oxidative stress, while Kats (catalase) were not. Several transporters were overabundant including one for dicarboxylates and one for branched amino acids. The present results confirm the centrality of nitrogenase in the actinorhizal symbiosis.

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“…It has been hypothesized that the increase of the proteolytic activity could play an essential role removing damaged proteins and release amino acids that would provide osmolytes to compensate the osmotic shock. (Frees et al, 2007; Ghedira et al, 2018).…”

Section: Resultsmentioning

confidence: 99%

Transcriptional response of the xerotolerant Arthrobacter sp. Helios strain to PEG-induced drought stress

Hernández‐Fernández

Galán

Carmona

et al. 2022

Preprint

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A new bacterial strain highly tolerant to desiccation and to UV radiation has been isolated from the microbiome of solar panels. This strain showed a high xerotolerance in the exponential and the stationary phase of growth and it has been classified as Arthrobacter sp. Helios according to its 16S rDNA, positioning this new strain in the Arthrobacter citreus group. The complete genome of Arthrobacter sp. Helios consists in a single circular chromosome of 3,895,998 bp, with a 66% GC content and no plasmids. A total of 3,586 genes were predicted, of which 2,275 protein-encoding genes were functionally assigned. The genome analysis suggests that it is motile, ecologically versatile, and capable of growing in a variety of carbon sources and well poised to respond to environmental stresses. Using PEG6000 to mimic arid stress conditions, we have studied the transcriptional response of this strain to matric stress when cells are cultured on media containing 10% (PEG10) and 35% PEG (PEG35). The transcriptomic analysis revealed that cells can be easily adapted to moderate matric stress (PEG10) by modifying the expression of a small number of genes to maintain a high growth rate, while a higher matric stress (PEG35) altered the expression of many more genes. Remarkably, these metabolic changes do not confer the cells a higher tolerance to desiccation, suggesting that mechanisms to support matric stress and desiccation tolerance are different. The peculiar observation that Arthrobacter sp. Helios seems to be permanently prepared to handle the desiccation stress makes it an exciting chassis for biotechnological applications.

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The PEG-responding desiccome of the alder microsymbiont Frankia alni

Cited by 16 publications

References 85 publications

Omics of the early molecular dialogue between Frankia alni and Alnus glutinosa and the cellulase synton

Omics of the early molecular dialogue between Frankia alni and Alnus glutinosa and the cellulase synton

The Proteogenome of Symbiotic Frankia alni in Alnus glutinosa Nodules

Transcriptional response of the xerotolerant Arthrobacter sp. Helios strain to PEG-induced drought stress

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