The performance of bone marrow mesenchymal stem cell – Implant complexes prepared by cell sheet engineering techniques

Zhou, Wei; Han, Chulhee; Song, Yingliang; Yan, Xingrong; Li, Dehua; Chai, Zhiguo; Feng, Zhihong; Dong, Yan; Liu, Liwen; Xie, Xing; Chen, Fulin; Zhao, Yimin

doi:10.1016/j.biomaterials.2010.01.036

Cited by 49 publications

(39 citation statements)

References 41 publications

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“…In the study of Zhou et al (44), multilayered rabbit BMSC sheets were assembled with two types of implant (surface-modified titanium and zirconia) to construct an MSC sheet-implant complex. After culturing in osteogenic medium for 2 weeks, two types of cell sheet-implant complex were transplanted under the skin of SCID mice for 8 weeks.…”

Section: Msc Sheets For Cartilage Integration and Repairmentioning

confidence: 99%

Application of the cell sheet technique in tissue engineering

Chen

Niu

et al. 2015

Biomedical Reports

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Section: Msc Sheets For Cartilage Integration and Repairmentioning

confidence: 99%

Application of the cell sheet technique in tissue engineering

Chen

Niu

et al. 2015

Biomedical Reports

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“…Microcomputed tomography is effective and reproducible for monitoring tumor-associated bone destruction, 31,32 analyzing the three-dimensional structure of scaffolds, 33 measuring subtle differences in bone growth into three-dimensional scaffolds, and calculating new bone deposition around implants. 34,35 In this study, the percent of bone volume/tissue volume of regenerated bone in the marrow bonding to the implants was calculated, and three-dimensional images were constructed. n-TiO 2 /PEEK showed higher bone volume/tissue volume than pure PEEK ( Figure 8).…”

Section: Figure 10mentioning

confidence: 99%

Nano-TiO2/PEEK bioactive composite as a bone substitute material: in vitro and in vivo studies

Liu²,

Wei³

et al. 2012

IJN

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Background: Compared with titanium (Ti) and other metal implant materials, poly(ether-ether ketone) (PEEK) shows outstanding biomechanical properties. A number of studies have also reported attractive bioactivity for nano-TiO 2 (n-TiO 2 ). Methods: In this study, n-TiO 2 /PEEK nanocomposites were prepared, taking advantage of the unique properties of both PEEK polymer and n-TiO 2 . The in vitro and in vivo bioactivity of these nanocomposites was assessed against a PEEK polymer control. The effect of surface morphology or roughness on the bioactivity of the n-TiO 2 /PEEK nanocomposites was also studied. n-TiO 2 /PEEK was successfully fabricated and cut into disks for physical and chemical characterization and in vitro studies, and prepared as cylindrical implants for in vivo studies. Their presence on the surface and dispersion in the composites was observed and analyzed by scanning and transmission electron microscopy and X-ray photoelectron spectroscopy. Results: Bioactivity evaluation of the nanocomposites revealed that pseudopods of osteoblasts preferred to anchor at areas where n-TiO 2 was present on the surface. In a cell attachment test, smooth PEEK showed the lowest optical density value (0.56 ± 0.07) while rough n-TiO 2 /PEEK exhibited the highest optical density value (1.21 ± 0.34, P , 0.05). In in vivo studies, the percent bone volume value of n-TiO 2 /PEEK was approximately twice as large as that of PEEK (P , 0.05). Vivid three-dimensional and histologic images of the newly generated bone on the implants further supported our test results. Conclusion: Our study demonstrates that n-TiO 2 significantly improves the bioactivity of PEEK, especially if it has a rough composite surface. A n-TiO 2 /PEEK composite with a rough surface could be a novel alternative implant material for orthopedic and dental applications.

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“…*, P < 0.05. able approaches for the repair or regeneration of damaged bone [22,23]. Tissue engineering and regenerative medicine have shown great promise in promoting bone repair using MSC sheets alone or in combination with implants wrapped in MSC sheets [13,24]. Compared with the traditional method of directly seeding cells in scaffolds, the cell sheet technique makes it possible to deliver a much larger number of cells for efficient tissue regeneration [9].…”

Section: Discussionmentioning

confidence: 99%

Restoration of rat calvarial defects by poly(lactide-co-glycolide)/hydroxyapatite scaffolds loaded with bone mesenchymal stem cells and DNA complexes

Wang

Guo

et al. 2011

Chin. Sci. Bull.

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A composite construct comprising of a bone mesenchymal stem cell (BMSC) sheet, plasmid DNA, encoding human bone morphogenic protein-2 (hBMP-2), and poly(lactide-co-glycolide)/hydroxyapatite (PLGA/HA) sponge was designed and employed in the restoration of rat calvarial defects. To improve gene transfection efficiency, a cationic chitosan derivative, N,N,N,-trimethyl chitosan chloride (TMC), was employed as the vector. The TMC/DNA complexes had a transfection efficiency of 13% in rat BMSCs, resulting in heterogeneous hBMP-2 expression in a 10-d culture period in vitro. In vivo culture of the composite constructs was performed by implantation into rat full-thickness calvarial defects, using constructs lacking pDNA-hBMP-2 or BMSC sheets as controls. Significantly higher heterogeneous expression of hBMP-2 was detected in vivo at 2 weeks for the cell sheet/DNA complex/scaffold constructs, compared with the constructs lacking pDNA-hBMP-2 or BMSC sheets. New bone formation was evident as early as 4 weeks in the experimental constructs. At 8 weeks, partial bridging of calvarial defects was observed in the experimental constructs, which was significantly better than the constructs lacking pDNA-hBMP-2 or BMSC sheets. Therefore, the combination of the PLGA/HA scaffold with BMSC sheets and gene therapy vectors is effective at enhancing bone formation.gene therapy, bone regeneration, bone marrow stem cells, poly(lactide-co-glycolide), BMP-2 Citation:Li D, Wang W, Guo R, et al. Restoration of rat calvarial defects by poly(lactide-co-glycolide)/hydroxyapatite scaffolds loaded with bone mesenchymal stem cells and DNA complexes.

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The performance of bone marrow mesenchymal stem cell – Implant complexes prepared by cell sheet engineering techniques

Cited by 49 publications

References 41 publications

Application of the cell sheet technique in tissue engineering

Application of the cell sheet technique in tissue engineering

Nano-TiO2/PEEK bioactive composite as a bone substitute material: in vitro and in vivo studies

Restoration of rat calvarial defects by poly(lactide-co-glycolide)/hydroxyapatite scaffolds loaded with bone mesenchymal stem cells and DNA complexes

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