The Phage Display Technique: Advantages and Recent Patents

Almeida, Síntia; Magalhaes, Aryane Aparecida C.; Soares, Siomar de Castro; Zurita-Turk, Meritxell; Goulart, Luiz Ricardo; Miyoshi, Anderson; Azevedo, Vasco

doi:10.2174/187221511796392060

Cited by 5 publications

(5 citation statements)

References 143 publications

(141 reference statements)

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“…tolerancia de los fagos a las altas temperaturas y ambientes ácidos, permitiendo seleccionar Nac con resistencia a estas condiciones (Muyldermans, 2013). Sin embargo, una desventaja es que al estar el Nac unido a la proteína III, existe la posibilidad de que ocurra un impedimento estérico (Silva et al, 2011).…”

Section: Técnicas De Despliegue De Nanoanticuerpos En Fagos Ribosomas Y Levadurasunclassified

Nanoanticuerpos: desarrollo biotecnológico y aplicaciones

et al. 2021

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Los anticuerpos monoclonales son una de las herramientas más revolucionarias en el área de la biomedicina por tener aplicaciones inmunoterapéuticas e inmunodiagnósticas. Sin embargo, el rápido avance tecnológico que demandan estas áreas genera la exploración de nuevas biomoléculas. El descubrimiento de anticuerpos compuestos únicamente por cadenas pesadas, presentes de forma natural en el suero de los camélidos y en algunas especies de tiburón, son motivo de estudio desde las últimas décadas como una alternativa a los anticuerpos convencionales. Estos poseen una región de reconocimiento antigénico, que consiste en un dominio variable por cada cadena, conocidos como anticuerpos de un solo dominio o nanoanticuerpos. Estas biomoléculas se caracterizan por tener un tamaño reducido, alta especificidad, estabilidad y bajo costo en su producción; propiedades que las convierten en una herramienta altamente versátil. En la presente revisión se abordarán aspectos relevantes de los nanoanticuerpos, como su descubrimiento, características estructurales, desarrollo en el campo de la biotecnología y su potencial de aplicación en enfermedades como el cáncer y en la identificación de microorganismos.

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Section: Técnicas De Despliegue De Nanoanticuerpos En Fagos Ribosomas Y Levadurasunclassified

Nanoanticuerpos: desarrollo biotecnológico y aplicaciones

et al. 2021

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“…Currently, the dogma of endolysins that are effective only against Gram-positive bacteria failed, and researches have focused based on endolysins for the control of Gramnegative and intracellular pathogens; furthermore, endolysin can suggest for prevention of bacterial infections (63,64).…”

Section: Future Perspective Of Endolysinmentioning

confidence: 99%

“…We already know that endolysins that are used in patients should not be any real challenges, for example, the product of Staphefekt SA.100 (Micreos, Bilthoven, The Netherlands) is registered as a medical device for treatment of eczema, rosacea, and acne, that is designed as constructed endolysin, which only targets the S. aureus and MRSA (64).…”

Section: What Challenges Do We Face For Treatments With Endolysin?mentioning

confidence: 99%

“…For instance, due to the fact that endolysins are very large molecules, they may be not only stimulate the immune system response and cause prevention of activity endolysin, but can also not enter into the cells and are not useful in the case of intracellular infections. Therefore, they should be used as a recombinant protein, for example truncated endolysins or use by technique of phage display (64).…”

Section: What Challenges Do We Face For Treatments With Endolysin?mentioning

confidence: 99%

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Endolysins of Bacteriophages as an Anti-Methicillin Resistant Staphylococcus aureus Infection in Children: A Narrative Review

Rahimzadeh¹,

Gill²,

Rezai³

2017

J Pediatr Rev

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Context: Spread of Methicillin-resistant Staphylococcus aureus (MRSA) can cause serious and sometimes fatal diseases especially in children. Outbreaks and increasing the prevalence of antibiotic-resistant MRSA at pediatric hospital calls for the development of novel preservation techniques. Endolysins and bacteriophages have been used successfully to control bacterial infections in children. Endolysins were considered as a useful treatment especially for the pathogens without disturbing the normal flora, the low chance of bacterial resistance, and their ability to kill colonizing pathogens on mucosal surfaces. Herein, we aimed to review the effectiveness of endolysins of bacteriophage for controlling methicillin-resistant Staphylococcus aureus infections in children.Evidence Acquisition: This review was performed by searching studies indexed in international databases including PubMed, Scopus, Web of Science, Science Direct, as well as Google Scholar published from 2000 until 2016.Results: Experimental data show that endolysins of Staphylococcus aureus bacteriophage can be used to combat methicillinresistant Staphylococcus aureus infections in children. Conclusions:Endolysins of bacteriophages could be effective for controlling a variety of methicillin-resistant Staphylococcus aureus infections.

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“…Phage display as a new tool for developing modern vaccines demonstrates a unique application of bacteriophages in vaccine design and is influenced by the immunomodulatory effects of phage exposure (summarized in Table 1). The premise of phage-display vaccination exploits the capability of certain phages to display a specific antigenic peptide or protein on their surface via a translational fusion with capsid protein(s) [30]. Alternatively, phages displaying peptide libraries can be screened with a specific antiserum to isolate novel protective antigens or mimotopes-peptides that mimic the secondary structure and antigenic properties of a protective carbohydrate, protein, or lipid, despite possessing a different primary structure.…”

Section: Phage Vaccinementioning

confidence: 99%

Immunocompatibility of Bacteriophages as Nanomedicines

Kaur

Nafissi

Wasfi

et al. 2012

Journal of Nanotechnology

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Bacteriophage-based medical research provides the opportunity to develop targeted nanomedicines with heightened efficiency and safety profiles. Filamentous phages also can and have been formulated as targeted drug-delivery nanomedicines, and phage may also serve as promising alternatives/complements to antibiotics. Over the past decade the use of phage for both the prophylaxis and the treatment of bacterial infection, has gained special significance in view of a dramatic rise in the prevalence of antibiotic resistance bacterial strains. Two potential medical applications of phages are the treatment of bacterial infections and their use as immunizing agents in diagnosis and monitoring patients with immunodeficiencies. Recently, phages have been employed as gene-delivery vectors (phage nanomedicine), for nearly half a century as tools in genetic research, for about two decades as tools for the discovery of specific target-binding proteins and peptides, and for almost a decade as tools for vaccine development. As phage applications to human therapeutic development grow at an exponential rate, it will become essential to evaluate host immune responses to initial and repetitive challenges by therapeutic phage in order to develop phage therapies that offer suitable utility. This paper examines and discusses phage nanomedicine applications and the immunomodulatory effects of bacteriophage exposure and treatment modalities.

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The Phage Display Technique: Advantages and Recent Patents

Cited by 5 publications

References 143 publications

Nanoanticuerpos: desarrollo biotecnológico y aplicaciones

Nanoanticuerpos: desarrollo biotecnológico y aplicaciones

Endolysins of Bacteriophages as an Anti-Methicillin Resistant Staphylococcus aureus Infection in Children: A Narrative Review

Immunocompatibility of Bacteriophages as Nanomedicines

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