The photoluminescent graphene oxide serves as an acceptor rather than a donor in the fluorescence resonance energy transfer pair of Cy3.5–graphene oxide

Piao, Yunxian; Liu, Fei; Seo, Tae Seok

doi:10.1039/c1cc15043j

Cited by 43 publications

(32 citation statements)

References 22 publications

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“…This has been used to perform DNA-directed assembly of GO sheet [108], and to modulate DNA to GO distance [109]. However, this interaction is still not strong enough, and thus displacement might take place.…”

Section: Covalently Linked Probesmentioning

confidence: 99%

Fluorescent sensors using DNA-functionalized graphene oxide

et al. 2014

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In the past few years, graphene oxide (GO) has emerged as a unique platform to develop DNA-based biosensors. This takes advantage of the DNA adsorption and fluorescence quenching property of GO.The adsorbed DNA probes can be desorbed from the GO surface in the presence of target analytes, producing fluorescence signal. In addition to this initial design, many other strategies have been reported including the use of aptamers, molecular beacons, and DNAzymes as probes, label-free detection, using the intrinsic fluorescence of GO and covalently linked DNA probes. The potential applications of DNA-functionalized GO range from environmental monitoring, cell imaging and biomedical diagnosis. In this review, we first summarize the fundamental surface interactions between DNA and GO and the related fluorescence quenching mechanism. Following that, the various sensor design strategies are critically compared. Problems at the current stages are described and a few future directions are also discussed.

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Section: Covalently Linked Probesmentioning

confidence: 99%

Fluorescent sensors using DNA-functionalized graphene oxide

et al. 2014

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“…In this case, the GO surface not only played a role as a binding platform but also as an energy acceptor9101112 to quench the fluorescence of IgG-FITC910. Since quenching occurs in close proximity13, only the IgG-FITCs that were adsorbed on antibody-conjugated GO would be quenched1415161718. As the number density of the binding sites was limited on the modified GO, more adsorbed analyte IgG proteins resulted in a stronger fluorescence signal from free IgG-FITCs in the solutions.…”

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confidence: 99%

Quantitative Fluorescence Quenching on Antibody-conjugated Graphene Oxide as a Platform for Protein Sensing

Huang

Shi

et al. 2017

Sci Rep

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We created an immunosensing platform for the detection of proteins in a buffer solution. Our sensing platform relies on graphene oxide (GO) nanosheets conjugated with antibodies to provide quantitative binding sites for analyte proteins. When analyte proteins and standard fluorescein-labelled proteins are competing for the binding sites, the assay exhibits quantitative fluorescence quenching by GO for the fluorescein-labelled proteins as determined by the analyte protein concentration. Because of this mechanism, measured fluorescence intensity from unquenched fluorescein-labelled protein was shown to increase with an increasing analyte protein concentration. As an alternative to the conventional enzyme-linked immunosorbent assay (ELISA), our method does not require an enzyme-linked second antibody for protein recognition and the enzyme for optical signal measurement. Thus, it is beneficial with its low cost and fewer systematic errors caused by the series of antigen-antibody recognition steps in ELISA. Immune globulin G (IgG) was introduced as a model protein to test our method and our results showed that the limit of detection for IgG was 4.67 pmol mL−1 in the buffer solution. This sensing mechanism could be developed into a promising biosensor for the detection of proteins, which would broaden the spectrum of GO applications in both analytical biochemistry and clinical diagnosis.

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“…[37][38][39][40] Seo and co-workers recently measured the fluorescence intensity of Cy3.5 nearby GO separated by up to 18 base pairs (bp) of DNA, where immobilization was achieved via adsorption of a five-adenine overhang. [41] For a systematic study, we employed eight amino and 6-carboxyfluorescein (FAM)…”

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confidence: 99%

DNA‐Length‐Dependent Fluorescence Signaling on Graphene Oxide Surface

Huang

Liu

2012

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Graphene has attracted an immense amount of research interest due to its unique electrical, mechanical, optical, and surface properties. [1][2][3][4] In addition, with its superior fluorescence quenching and adsorption capacity, graphene has been increasingly used for making biosensors, [5][6][7][8] drug delivery vehicles, [9, 10] and imaging agents. [10,11] To disperse in an aqueous solution, graphene oxide (GO) with surface carboxyl and hydroxyl groups is often prepared.GO is also an excellent quencher for adsorbed fluorophores with quenching efficiency approaching 100%. [12][13][14] At the same time, GO selectively adsorbs non-structured and single-stranded DNA (ssDNA), [15,16] which can subsequently desorb upon forming double-stranded (dsDNA) or well-folded structures. Based on these understandings, many optical sensors have been designed for the detection of metal ions, [12,17,18] small molecules, [11, 19, 20] [14, 21] [6, 13, 22-24] proteins, and nucleic acids. For example, adsorption of a fluorophorelabeled probe DNA resulted in quenched fluorescence. In the presence of its complementary DNA (cDNA), the fluorescence was recovered due to duplex formation and subsequent desorption. In this This is the peer reviewed version of the following article: Huang, P.-J. J., & Liu, J. (2012 Submitted to -2 -process, the fluorophore-to-GO distance increased from zero to infinity to achieve the maximal fluorescence enhancement. In addition to GO, many other carbon based nanomaterials including carbon nanotubes, [25][26][27][28][29] mesoporous carbon, [30] carbon nanoparticles [31,32] and water soluble nano-C60 [33] have also been tested for similar applications.On the other hand, little is known about DNA length-dependent fluorescence quenching within a few nanometers from the GO surface. Such information is important for rational design of covalently linked probes. Compared to physisorbed probes, covalent sensors are reversible, regenerable, less prone to non-specific probe displacement, and allow continuous monitoring under flow conditions. [34] In addition, studying distance-dependent fluorescence properties are crucial for the fundamental understanding of DNA/GO interaction and its quenching mechanism. Theoretical calculations predicted that quenching by graphene follows a d -4 dependency, where d is the fluorophore-to-graphene distance. [35,36] This is formally similar to the so-called nanosurface energy transfer (NSET) studied using gold nanoparticles as quencher. [37][38][39][40] Seo and co-workers recently measured the fluorescence intensity of Cy3.5 nearby GO separated by up to 18 base pairs (bp) of DNA, where immobilization was achieved via adsorption of a five-adenine overhang.[41]For a systematic study, we employed eight amino and 6-carboxyfluorescein (FAM) dual-labeled DNAs with varying FAM positions (see Figure 1D for the DNA sequences). TheDNAs were respectively reacted with GO in the presence of EDC to form covalent amide linkages ( Figure 1A, step 1), such that the FAM and GO were separated by...

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The photoluminescent graphene oxide serves as an acceptor rather than a donor in the fluorescence resonance energy transfer pair of Cy3.5–graphene oxide

Cited by 43 publications

References 22 publications

Fluorescent sensors using DNA-functionalized graphene oxide

Fluorescent sensors using DNA-functionalized graphene oxide

Quantitative Fluorescence Quenching on Antibody-conjugated Graphene Oxide as a Platform for Protein Sensing

DNA‐Length‐Dependent Fluorescence Signaling on Graphene Oxide Surface

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