The phylogenetic relationship of the Q9-equipped species of the heterobasidiomycetous yeast genera Rhodosporidium and Leucosporidium based on the partial sequences of 18S and 26s ribosomal ribonucleic acids: The proposal of a new genus Kondoa.

Abstract

Eight strains of the Q9-equipped heterobasidiomycetous yeast species, Rhodosporidium ma/vine//urn, Rhodosporidium toruloides, and Leucosporidium scottii and the Q9-equipped anamorphic yeast species, Rhodotorula lactosa were examined as to the partial sequence determinations of 18S rRNA and 26S rRNA. The positions determined were 1451 through 1618 of 18S rRNA and 492 through 625 of 26S rRNA. The two determinations of the partial sequences of 18S rRNA and 26S rRNA showed that the four species mentioned above constituted their own separate clusters. Rhodosporidium ma/vine//urn occupied a unique situation. The phylogenetic relationship between R. ma/vine//urn and R. toru/oides was more distant than that between R. toruloides and L. scottii. The data indicated that these three heterobasidiomycetous yeast species are distinguished from each other at the generic level. The new genus Kondoa was proposed for R. ma/vine//um.

Molecular approaches to the taxonomy of ballistosporous yeasts based on the analysis of the partial nucleotide sequences of 18S ribosomal ribonucleic acids.

“…These data will appear in the DDBJ, EMBL and GenBank Nucleotide Sequence Databases with the numbers of D10796, D10797, D10775 and D10800, respectively. Data on non-ballistosporous species, except for E. hasegawianum, were those reported in the literature (10,75,77,(83)(84)(85)(86) and are shown in Table 2 with several key characteristics.…”

“…39 but Kon. malvinella has that of AUUAUU-- (85), in which one nucleotide is substituted. Yamada et al (85) established a new genus Kondoa for Rhodosporidium malvinellum, who has Q-9 as the major ubiquinone differing from the other species of the genus, based on the analysis of the partial sequences of 18S and 26S rRNAs and the morphological characteristic found in basidium.…”

“…The mode of conidiogenesis found in ballistosporous yeasts became somewhat complicated by 18S rRNA of Ballistosporous Yeasts 109 the finding of new genera and a question has arisen on the taxonomic significance of this characteristic (27). Recently, several researchers discussed on the phylogenetic relationships among basidiomycetous yeasts based on the partial nucleotide sequences of 18S and 25/265 rRNAs (10,11,75,77,(83)(84)(85)(86). However, ballistosporous yeasts have not been included in such studies except for a teleomorphic species, Sporidioholus johnsonii (10).…”

Phylogeny among the basidiomycetous yeasts inferred from small subunit ribosomal DNA sequence

“…Among these, the genera Cystojilobasidium (Obenvinkler et al, 1983), Mrakia (Yamada & Komagata, 1987), and Kondoa (Yamada et al, 1989) the genera Leucosporidium and Rhodosporidium in the teliospore-forming yeasts, and included within the Filobasidiaceae. Hamamoto et al (1988Hamamoto et al ( , 1991 proposed the genus Erythrobasidium in the Filobasidiaceae for the teleomorph of Rhodotorula hasegawae.…”

Significance of the coenzyme Q system in the classification of yeasts and yeast-like organisms. XLVII. The phylogenetic relationships of some heterobasidiomycetous yeast species based on the partial sequences of 18S and 26S ribosomal RNAs.

“…The dendrogram was drawn by the simple linkage method. The number of base differences was calculated from the sequence data obtained presently and previously (15,22). *Type strain.…”