Significance of the coenzyme Q system in the classification of yeasts and yeast-like organisms. XLVII. The phylogenetic relationships of some heterobasidiomycetous yeast species based on the partial sequences of 18S and 26S ribosomal RNAs.

Abstract

Eight strains of Leucosporidium fellii, L. lari-marini, Filobasidium capsuligenum, F floriforme, and F uniguttulatum were examined for partial base sequences in positions 492-625 (134 bases) of 26S rRNA and in positions 1451-1618 (168 bases) of 18S rRNA. In the 26S rRNA partial base sequence, there were low maximum homologies (46-58%) among L. fellii, L. lari-marini, and Filobasidium species. In the 18S rRNA partial base sequence, F capsuligenum had 4 and 5 base differences, compared with F floriforme and F uniguttulatum, respectively. Leucosporidium lari-marini and L. fellii showed 0 and 4 base differences with Cystofilobasidium capitatum and L. scottii, respectively. The sequence data obtained were discussed phylogenetically and taxonomically, especially on transferring L. lari-marini to the genus Cystofilobasidium.Leucosporidium fellii Gimenez-Jurado et van Uden (5) and Leucosporidium lari-marini Saez et Nguyen (11) were recently described as a seventh and an eighth species of the genus Leucosporidium Fell, Statzell, Hunter et Phaff (2). The former species is characterized morphologically by self-sporulating and forming mycelia with clamp connections, and physiologically by assimilating L-tartarate, and the latter species is similar physiologically to L. stokesii. Previously, we proposed the i Significance of the coenzyme Q system in the classification of yeasts and yeast -like organisms.

Taxonomic studies in the Microbotryomycetidae: Leucosporidium golubevii sp. nov., Leucosporidiella gen. nov. and the new orders Leucosporidiales and Sporidiobolales

“…On the contrary, in the BIONJ analysis (Fig. With respect to other taxonomic criteria, whereas L. fellii and M. intermedium have CoQ 9 (YAMADA & NAKAGAWA, 1992;NAKASE & SUZUKI, 1986), Microbotryum and Sphacelotheca have CoQ 10 (PRIL- LINGER et al 1991) as most species of the Microbotryomycetidae studied to date. The association of L. fellii and M. intermedium with Microbotryum and allied genera in the MCMC tree contrasts with the absence of colacosomes and the phytoparasitic life strategy of the Microbotryales.…”

An emendation of the genus Debaryomyces Lodder et Kreger-van Rij and the proposals of two new combinations, Debaryomyces carsonii and Debaryomyces etchellsii (Saccharomycetaceae).

“…For Part XLVII, see ref. (9 the genus, D. hansenii (member of our 1st subgroup). We also pointed out that D. melissophilus had relatively higher base substitutions in its 26S (or 25S) rRNA, compared with other Debaryomyces species (12).…”