So far, Rice production has yet to meet domestic demand due to population growth, which continues to increase yearly. Lowland rice provides the most significant contribution to national rice production; for this reason, upland rice development is urgently needed to help increase national rice production. Central Sulawesi's upland rice germplasm is very abundant; it's just that there are still many that still need to be identified. For this reason, this study aims to identify the chemical levels of rice in several local upland rice lines. This research was conducted at the Research and Development Site of Gogo Rice, Faculty of Agriculture, Tadulako University is located at 230 meters above sea level in Kalama Hamlet III, Tamarenja Village, Sindue Tobata District, Donggala Regency, Central Sulawesi Province, with coordinates LS 00o26'51.4 BT 119o49'50,5. This research started from January to June 2023. This research was compiled using the Randomized Block Design (RAK) method with 14 local upland rice lines (Pae bohe, Menso, Maraki, Uva buya, Kalendeng, Pulau tau leru, Pulu Konta, Jahara, Buncaili, Delima, Pae dupa, Puyu tas, Tako, Dongan) and one variety of check (Situ bagendit) which was repeated three times to obtain 45 experimental units. The variables observed included moisture content, ash, protein, fat, carbohydrates, fibre and amylose. The results showed that the Pae dupa strain yielded a water content of 7.44%, protein content of 8.91%, fibre content of 3.90% and amylose content of 12.58%, while the Situ bagendit strain had a moisture content of 11.61%, ash content of 0.57%, protein content of 7.72%, fat content 0.66%.