The Physical Role of Mesenchymal Cells Driven by the Actin Cytoskeleton Is Essential for the Orientation of Collagen Fibrils in Zebrafish Fins

Kuroda, Junpei; Itabashi, Takeshi; Iwane, Atsuko H.; Aramaki, Toshihiro; Kondo, Shigeru

doi:10.3389/fcell.2020.580520

Cited by 11 publications

(4 citation statements)

References 53 publications

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“…They are located adjacent to apical epidermal cells in the fin fold with distally extended tree-like processes. Recent work has shown that fin mesenchymal cells guide fin formation by aligning collagen fibers of actinotrichia (Kuroda et al, 2020). Genetic ablation of fin mesenchymal cells in the pectoral and median fin folds results in collapse of the respective fins (Lalonde and Akimenko, 2018).…”

Section: Discussionmentioning

confidence: 99%

Origin and diversification of fibroblasts from the sclerotome in zebrafish

Roger

Huang

2021

Preprint

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Fibroblasts play an important role in maintaining tissue integrity by secreting components of the extracellular matrix and initiating response to injury. Although the function of fibroblasts has been extensively studied in adults, the embryonic origin and diversification of different fibroblast subtypes during development remain largely unexplored. Using zebrafish as a model, we show that the sclerotome, a sub-compartment of the somite, is the embryonic source of multiple fibroblast populations, including tenocytes (tendon fibroblasts), blood vessel associated fibroblasts, and fin mesenchymal cells. High resolution imaging shows that different fibroblast subtypes occupy unique anatomical locations with distinct morphologies. Photoconversion-based cell lineage analysis reveals that sclerotome progenitors at different dorsal-ventral and anterior-posterior positions display distinct differentiation potentials. Single cell clonal analysis suggests that sclerotome progenitors are multipotent, and the fate of their daughter cells is biased by their migration paths and relative positions. Using a small molecule inhibitor, we show that BMP signaling is required for the development of fin mesenchymal cells in the peripheral fin fold. Together, our work demonstrates that the sclerotome contains multipotent progenitors that respond to local signals to generate a diverse population of tissue-resident fibroblasts.

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Section: Discussionmentioning

confidence: 99%

Origin and diversification of fibroblasts from the sclerotome in zebrafish

Roger

Huang

2021

Preprint

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“…Overall, regenerated tissues in CF animals consistently showed unaligned fibers compared to control, and were more prone to develop aberrant fiber extrusions into the intra-fiber space ( Figure 6N ). Interestingly, while mesenchymal cells are essential to guide the collagen fibers organization in zebrafish fins (38), we observed that disrupted fiber content and orientation coincided with an abnormal mesenchymal cell distribution and shape ( Figure S13C ). Remarkably, all these aberrant phenotypes are rescued by curcumin treatment ( Figures 6; S13 ).…”

Section: Resultsmentioning

confidence: 96%

Curcumin-mediated NRF2 induction limits inflammatory damage in preclinical models of cystic fibrosis

Leon-Icaza,

Fretaud,

Cornélie

et al. 2024

Preprint

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Overactive inflammation is directly correlated with airway damage and early death in individuals with cystic fibrosis (CF), a genetic disorder caused by mutation in the CFTR gene. Reducing the impact of inflammatory damage is therefore a major concern in CF. Several studies indicate that a decrease in the nuclear factor erythroid 2-related factor-2 (NRF2) signaling in people with CF may hamper their ability to alleviate oxidative stress and inflammation, although the role of NRF2 in CF inflammatory damage has not been determined. Therefore, we examined whether the phytochemical curcumin, an activator of NRF2, might provide a beneficial effect in the context of CF. Herein, combining Cftr-depleted zebrafish larvae as innovative biomedical model with CF patient-derived airway organoids (AOs), we sought to understand how NRF2 dysfunction leads to abnormal inflammatory status and impaired tissue remodeling, and determine the effects of curcumin in reducing inflammation and tissue damage in CF. We demonstrate that NFR2 is instrumental in efficiently regulating inflammatory and repair processes in vivo, thereby preventing acute neutrophilic inflammation and tissue damage. Importantly, curcumin treatment restores NRF2 activity in both CF zebrafish and AOs. Curcumin reduces neutrophilic inflammation in CF context, by rebalancing the production of epithelial ROS and pro-inflammatory cytokines. Furthermore, curcumin alleviates CF-associated tissue remodeling and allows tissue repair to occur. Our findings demonstrate that curcumin reduces inflammatory damage by restoring normal NRF2 activity, since disruption of Nrf2 pathway abrogated the effect of treatment in CF zebrafish. This work highlights the protective role of NRF2 in limiting inflammation and injury, and show that therapeutic strategies to normalize NRF2 activity using curcumin might simultaneously reduce inflammation and enhance tissue repair, and thus prevent infectious and inflammatory lung damage in CF.

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“…This suggests that LMFF reductions are passive and do not drive the emergence of median n primordia. We postulate that regression or degradation of the supporting material in the LMFF structure, such as actinotrichia and laminin [38][39][40][41] , caused the epithelial migration in the trunk at the inter-n area. Further molecular developmental biological studies are required to reveal how this collective epithelial cell movement occurs.…”

Section: Discussionmentioning

confidence: 99%

Developmental Independence of Median Fins From the larval Fin Fold Revises Their Evolutionary Origin

Miyamoto

Kawakami

Tamura

et al. 2022

Preprint

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The median fins in modern fish that show separated forms (dorsal, anal, and caudal fins) are derived from a continuous fold-like structure, both in ontogeny and phylogeny. The median fin fold (MFF) hypothesis assumes that the median fins evolved by reducing some positions in the continuous fin fold of basal chordates, based on the classical morphological observation of developmental reduction in the larval fin folds of living fish. However, the developmental processes of median fins are still unclear at the cellular and molecular levels. Here, we describe the transition from the larval fin fold into the median fins in zebrafish at the cellar and molecular developmental level. We demonstrate that reduction does not play a role in the emergence of the dorsal fin primordium: the reduction occurs passively after the primordium formation, rather than actively scrapping the non-fin forming region by inducing cell death. We also report that the emergence of specific mesenchymal cells and their proliferation promote the dorsal fin primordium formation. Based on these results, we propose a revised hypothesis of median fin evolution in which acquisition of de novo developmental mechanisms is a crucial evolutionary component of the separated forms of median fins.

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The Physical Role of Mesenchymal Cells Driven by the Actin Cytoskeleton Is Essential for the Orientation of Collagen Fibrils in Zebrafish Fins

Cited by 11 publications

References 53 publications

Origin and diversification of fibroblasts from the sclerotome in zebrafish

Origin and diversification of fibroblasts from the sclerotome in zebrafish

Curcumin-mediated NRF2 induction limits inflammatory damage in preclinical models of cystic fibrosis

Developmental Independence of Median Fins From the larval Fin Fold Revises Their Evolutionary Origin

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