The physicochemical and biological characterization of a 24-month-stored nanocomplex based on gold nanoparticles conjugated with cetuximab demonstrated long-term stability, EGFR affinity and cancer cell death due to apoptosis

Andrade, Lídia M.; Martins, Estefânia M.N.; Versiani, Alice F.; Reis, Daniela Silva; Fonseca, Flávio Guimarães da; Souza, Ívina P. de; Paniago, R.; Pereira-Maia, Elene C.; Ladeira, Luiz Orlando

doi:10.1016/j.msec.2019.110203

Cited by 23 publications

(13 citation statements)

References 44 publications

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“…In recent years, an increasing number of studies were performed on the interaction of cetuximab-conjugated AuNPs (cetuximab-AuNPs) against EGFR-overexpressing cancers. It was shown that cetuximab-AuNPs displayed enhanced EGFR endocytosis and the subsequent suppression of downstream signaling pathway, leading to the inhibition of cell proliferation and the acceleration of apoptosis compared to AuNPs or cetuximab alone [ 28 , 29 , 30 , 31 ]. These findings suggest that AuNPs have promising potential to be used as drug carriers to increase the therapeutic efficiency of monoclonal antibodies.…”

Section: Introductionmentioning

confidence: 99%

Effect of Cetuximab-Conjugated Gold Nanoparticles on the Cytotoxicity and Phenotypic Evolution of Colorectal Cancer Cells

2021

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Epidermal growth factor receptor (EGFR) is estimated to be overexpressed in 60~80% of colorectal cancer (CRC), which is associated with a poor prognosis. Anti-EGFR targeted monoclonal antibodies (cetuximab and panitumumab) have played an important role in the treatment of metastatic CRC. However, the therapeutic response of anti-EGFR monoclonal antibodies is limited due to multiple resistance mechanisms. With the discovery of new functions for gold nanoparticles (AuNPs), we hypothesize that cetuximab-conjugated AuNPs (cetuximab-AuNPs) will not only improve the cytotoxicity for cancer cells, but also introduce expression change of the related biomarkers on cancer cell surface. In this contribution, we investigated the size-dependent cytotoxicity of cetuximab-AuNPs to CRC cell line (HT-29), while also monitored the expression of cell surface biomarkers in response to treatment with cetuximab and cetuximab-AuNPs. AuNPs with the size of 60 nm showed the highest impact for cell cytotoxicity, which was tested by cell counting kit-8 (CCK-8) assay. Three cell surface biomarkers including epithelial cell adhesion molecule (EpCAM), melanoma cell adhesion molecule (MCAM), and human epidermal growth factor receptor-3 (HER-3) were found to be expressed at higher heterogeneity when cetuximab was conjugated to AuNPs. Both surface-enhanced Raman scattering/spectroscopy (SERS) and flow cytometry demonstrated the correlation of cell surface biomarkers in response to the drug treatment. We thus believe this study provides powerful potential for drug-conjugated AuNPs to enhance cancer prognosis and therapy.

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Section: Introductionmentioning

confidence: 99%

Effect of Cetuximab-Conjugated Gold Nanoparticles on the Cytotoxicity and Phenotypic Evolution of Colorectal Cancer Cells

2021

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“…Also, nanoparticles have the advantage of releasing metal ions slowly, making it possible to distribute a continuous dose in the organism. [29,56] Previous reports also proved the enhanced bioactivity against bacteria has been observed for the tested nanosized ternary complexes. [8,9,11] Similarly, Raydan et al [6] results suggest that Cu -Amino acid complexes have potential as antibacterial agents.…”

Section: Antimicrobial Activitymentioning

confidence: 67%

“…[9,26] Nevertheless, literature has not afforded a clear example of the synthesis of nanoscale ternary complexes especially in dimer form although; few researches have alleged to have done so. [26][27][28][29] In this study, Co and Cu mixed ligand complexes in bulk and nanoscale were prepared. All these complexes were characterized and investigated.…”

Section: Introductionmentioning

confidence: 99%

Macro‐ and nano‐oligomers ternary metal complexes preparation, structural elucidation: Antimicrobial, anticancer activities, and mechanistic study of Cu nanocomplexes on liver carcinoma

Rabie

Khalil

Elaasser

et al. 2021

Applied Organom Chemis

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A new binuclear transition metal complexes of Co (II) and Cu (II) have been synthesized in both bulk and nanosized formations. The complexes were synthesized by the reaction between equimolar quantities of the metal sulfate with nitrilotriacetic acid and creatine. The nanosized forms are controlled by using (CTAB). Characterization of the nanocomplexes has been done using a transmission electron microscope (TEM) and X‐ray diffraction. This technique is considered a novel approach to the synthesis of the nanoternary complexes. The Co (II) and Cu (II) complexes displayed distorted dimeric octahedral and tetrahedral structures, respectively. The prepared metal complexes were investigated by electronic spectral analysis, magnetic susceptibility measurement, infrared spectra, thermal gravimetric analysis (TGA), UV–visible spectra, and mass spectra. The molecular weights of the dimer complexes are measured by GPC (Gel Permeation Chromatography). Characterization of the obtained complexes showed that the NTA ligand coordinates with metal ions in tridentate mode (one N and two COO−) after deprotonation, the creatine molecule coordinates through the carboxylate group, while the dimeric structure of these complexes formed with one of the sulfate groups which coordinates as a bridged bidentate ligand. Investigation of the biological activity of these complexes, as well as their hepatocellular carcinoma (HepG2) cell line inhibitory, has also been achieved.

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“…In Brazil, concerning to the therapeutic area, the research focuses on the application of metallic nanoparticles, metallic oxide, proteins, and carbons nanoparticles and on the development of biosensors to diagnose endemic infectious diseases such as the dengue virus (Basso et al, 2018) and Plasmodium vivax (Regiart et al, 2021). Moreover, there is the development of nanovaccines for the Zika virus (Favaro et al, 2021) and treatments for fungal infections using drug-delivery systems (Arias et al, 2020) and biological silver NP (Bocate et al, 2019;Valente et al 2020;Cavali et al, 2021) in addition to the development of nanoparticles against carcinogenic cells, stimulating the apoptosis process (Andrade et al, 2020).…”

Section: Graphical Abstractsmentioning

confidence: 99%

Nanotechnology on agent identification, diseases diagnosis and therapeutic approaches

Reis¹,

Thaler²,

Lustosa³

et al. 2022

biori

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The physicochemical and biological characterization of a 24-month-stored nanocomplex based on gold nanoparticles conjugated with cetuximab demonstrated long-term stability, EGFR affinity and cancer cell death due to apoptosis

Cited by 23 publications

References 44 publications

Effect of Cetuximab-Conjugated Gold Nanoparticles on the Cytotoxicity and Phenotypic Evolution of Colorectal Cancer Cells

Effect of Cetuximab-Conjugated Gold Nanoparticles on the Cytotoxicity and Phenotypic Evolution of Colorectal Cancer Cells

Macro‐ and nano‐oligomers ternary metal complexes preparation, structural elucidation: Antimicrobial, anticancer activities, and mechanistic study of Cu nanocomplexes on liver carcinoma

Nanotechnology on agent identification, diseases diagnosis and therapeutic approaches

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