The physiology of wound healing

Young, Alistair; McNaught, Clare-Ellen

doi:10.1016/j.mpsur.2011.06.011

Cited by 191 publications

(245 citation statements)

References 12 publications

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“…A ausência de diferença entre grupos tratados e controles pode estar relacionada com o processo de reparação fisiológica das feridas de pele, que se dá, de forma clássi-ca, em quatro fases bem definidas que se sobrepõem, incluindo hemostasia, inflamação, proliferação e, por último, remodelação (Enoch & Leaper 2007, Young & McNaught 2011. Schultz et al (2011) subdivide a terceira fase em migração e proliferação, e a quarta em contração e remodelação.…”

Section: Discussionunclassified

Quantificação de fatores de crescimento na pele de equinos tratada com plasma rico em plaquetas

et al. 2014

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ABSTRACT.-Souza M.V., Pinto J.O., Costa M.M., Santos E.C., Garcia S.L.R. & Oliveira L.L. 2014.[Quantification of growth factors in horse skin treated with platelet-rich plasma.] Quantificação de fatores de crescimento na pele de equinos tratada com plasma rico em plaquetas. Pesquisa Veterinária Brasileira 34(6):599-612. Departamento de Veterinária, Universidade Federal de Viçosa, Campus Universitário s/n, Viçosa, MG 36570-000, Brazil. E-mail: msouza@ufv.br Platelet-rich plasma (PRP) is a product derived from total blood centrifugation, rich in bioactive factors, such as growth factors. Despite largely used in healing processes, there is a controversy whether the therapy is effective in promoting skin healing. The objective of this study was to quantify and compare the concentrations of the factors TGF-β1 and PDGF-BB in PRP, blood plasma and skin, at different phases of the healing process of skin treated or not with PRP. Seven healthy crossbred 16 to 17-year-old geldings (16.14±0.63) were used. Three quadrangular-shaped lesions (6.25cm 2 ) were surgically induced into the right and left gluteal regions of all animals. Twelve hours after induction of the wounds, 0.5mL of the PRP was administered in each of the four edges of the wound in one of the gluteal regions (Treated group = TG) randomly chosen. The contralateral region was used as control (CG). The wounds were submitted to daily cleaning with Milli-Q water, and samples were obtained with a 6mm diameter biopsy Punch. Six skin biopsies were obtained, the first carried out immediately after the production of the wound (T0), and the others 1 (T1), 2 (T2), 7 (T3), and 14 (T4) days after the lesion was induced. The sixth biopsy (T5) was obtained after complete healing of the skin, which occurred at about day 37 (36.85±7.45, CG; 38.85±6.46, TG). EDTA blood samples were also obtained, at all the times mentioned. Quantification of TGF-β1 and PDGF-BB growth factors on the skin, PRP, and blood plasma was carried out by the ELISA technique. Data were statistically analyzed by the t test, Pearson correlation and regression, at a significance level of 5%. No difference was found between the groups in the values of the two growth factors measured on the skin, at the different times. Also, no correlation was found between the amount of growth factors present in the skin and plasma. On the other hand, a positive correlation was observed between PRP and skin in the treated group, for the growth factors TGF-β1 (r=0.31) and PDGF-BB (r=0.38), as well as between both growth factors present in PRP (r=0.81). Considering the growth factor concentrations at T0, the highest skin values (p<0.05) of TGF-β1, in both groups, occurred at T3 and T5. Higher values (p<0.05) of PDGF-BB occurred at T4 (TG) and T5 (CG). No plasma changes occurred at the concentration of these factors in relation to T0, suggest-1 Recebido em 5 de outubro de 2013.Aceito para publicação em 5 de junho de 2014.

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Section: Discussionunclassified

Quantificação de fatores de crescimento na pele de equinos tratada com plasma rico em plaquetas

et al. 2014

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“…Young and McNaught (30) state that a period of over two years may elapse before scar tissue reaches maturation; during this phase, there must be a balance between collagen synthesis and degradation through fibroblast collagenases, neutrophils and macrophages, which break the molecule into small fragments, to then be denatured and digested by other proteases.…”

Section: Discussionmentioning

confidence: 99%

Pythiosis cutánea en equinos tratados con acetonida de triamcinolona. Parte 3. Análisis histomorfométrico

2017

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Objetivo. El objetivo del estudio fue analizar histomorfométricamente el proceso de cicatrización en equinos con pythiosis tratados con acetonida de triamcinolona. Materiales y métodos. Se utilizaron 24 equinos con pythiosis, a un grupo se aplicó 50 mg de acetonida de triamcinolona intramuscular (GT), mientras que al otro grupo no fue aplicado tratamiento (GC). Fueron colectadas biopsias de tejido, procesadas, laminadas y coloreadas con hematoxilina eosina (H&E), tricrómico de Gómori (TG), picrosirius red/polarization (PR/P) y plata metanamina de Grocott (GMS). Fueron seleccionados y fotomicrografiados 10 campos histológicos, analizados con el software BioEstat 5.0, obteniéndose las cantidades tisulares de células como eosinófilos, neutrófilos, macrófagos, fibroblastos y colágeno a través de evaluación planimétrica por conteo de puntos. Resultados. En coloración de GSM, se observó disminución de hifas intralesionales del Pythium a los 16 días (p<0.05). En coloración de H&E, hubo disminución del proceso inflamatorio, evidenciado en eosinófilos (p=0.0001), neutrófilos (p=0,0001) y macrófagos (p=0.00001). En coloraciones de TG y PR/P, hubo aumento de fibroblastos, fibras colágenas y cambio progresivo de colágeno tipo III a tipo I, el aumento de fibroblastos fue significativo (p=0.0001) del día 16 al 40, la expresión de colágeno fue altamente significativa (p=0.0001) del día 16 hasta el final del estudio. Hubo correlación significativa entre neutrófilos y macrófagos (p=0.00018), colágeno y eosinófilos (p=0.03) y colágeno y fibroblastos (p=0.02). Los animales del GC no presentaron mejoría histomorfométrica durante el estudio. Conclusiones. Es posible concluir que la acetonida de triamcinolona produce recuperación histomorfométrica celular y tecidual en los equinos con pythiosis.

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“…Conversion of the granulation tissue to scar tissue is accomplished by remodelation of the extracellular matrix 16 . Wounds never reach the same level of tissue strength 17 .…”

Section: Wound Healing and Its Studymentioning

confidence: 99%

“…Wounds can be divided according to their healing time into acute and chronic 18 , where the healing process lasts longer than 12 weeks after the initial insult 17 . Another classification can be according to the mechanism of origin (mechanical -e.g.…”

Section: Wound Healing Models For the Skinmentioning

confidence: 99%

Models for the study of skin wound healing. The role of Nrf2 and NF-κB

Ambrožová¹,

Ulrichová²,

Galandáková³

2017

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub

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a,bNrf2 and NF-κB transcription factors act in wound healing via their anti-inflammatory and anti-oxidant effects or through the immune response. Studying this process is a matter of some importance given the high cost of wound treatment. A major contribution in this regard is being made by models that enable investigation of the involvement of multiple factors in wound healing and testing new curative substances. This literature review was carried out via searches in the PubMed and Web of Science databases up to 2016. It covers skin wound healing, available models for its study (part I), the role of Nrf2 and NF-κB, substances that influence them and whether they can be used as markers (part II). Was found that in vitro assays are used for their availability but a holistic view must be established in vivo. In silico approaches are facilitating assessment of a vast amount of research data. Nfr2 and NF-κB play a crucial and reciprocal role in wound healing. Nrf2 controls repair-associated inflammation and protects against excessive accumulation of ROS while Nf-κB activates the innate immune reaction, proliferation and migration of cells, modulates expression of matrix metalloproteinases, secretion and stability of cytokines and growth factors for wound healing.

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The physiology of wound healing

Cited by 191 publications

References 12 publications

Quantificação de fatores de crescimento na pele de equinos tratada com plasma rico em plaquetas

Quantificação de fatores de crescimento na pele de equinos tratada com plasma rico em plaquetas

Pythiosis cutánea en equinos tratados con acetonida de triamcinolona. Parte 3. Análisis histomorfométrico

Models for the study of skin wound healing. The role of Nrf2 and NF-κB

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