The Plant Homeodomain Protein Clp1 Regulates Fungal Development, Virulence, and Autophagy Homeostasis in Magnaporthe oryzae

Abstract

The fungal pathogen Magnaporthe oryzae causes serious diseases of grasses such as rice and wheat. Autophagy plays an indispensable role in the pathogenic process of M. oryzae .

Acyl-coenzyme A binding protein MoAcb1 regulates conidiation and pathogenicity in Magnaporthe oryzae

“…Autophagy is an evolutionarily conserved intracellular degradation process in which some damaged proteins or organelles are encapsulated by double-membrane autophagy vesicles, then delivered to lysosomes or vacuoles to be degraded and cycled (Zhou et al, 2018;Zhu et al, 2021), thereby fulfilling the metabolic needs of the cells and the renewal of certain organelles. According to the different substrates, autophagy can be divided into macroautophagy and selective autophagy (Wang et al, 2022). In Fusarium graminearum, autophagy is required for normal vegetative growth, complete virulence, and toxin biosynthesis (Lv et al, 2017).…”

Transcriptional Regulation of Autophagy-Related Genes by Sin3 Negatively Modulates Autophagy in Magnaporthe oryzae

“…It has been reported previously that Sin3A could be targeted to the nucleolus by interacting with SAP30L in mammals ( 43 ). Recently, MoCti6, a potential component of Rpd3-HDAC, was also reported to be involved in the regulation of autophagy ( 23 ). So far, no transcriptional factors which have the capability to bind DNA were identified to be involved in the Sin3-Hos2 complex and in the regulation of autophagy.…”

“…Vacuoles were observed with 405-nm laser excitation following 7-amino-4-chloromethylcoumarin (CMAC) staining for 30 min. To monitor the translocation of lipid droplets during appressoria formation, conidial suspensions were inoculated on the hydrophobic surfaces and stained with boron-dipyrromethene (BODIPY) solution (Invitrogen; D-3922) for 0, 4, 8, and 24 h before being photographed with a fluorescence microscope ( 23 ). Images were subjected to processing with ImageJ software.…”

“…Accumulating evidence indicates that autophagy is required for the growth and pathogenicity of phytopathogenic fungi ( 3 , 5 , 22 – 24 ). During the infection process of pathogens, autophagy is required for the formation and development of the infectious structure, thereby affecting the virulence of the pathogen ( 3 , 5 ).…”

MoCbp7, a Novel Calcineurin B Subunit-Binding Protein, Is Involved in the Calcium Signaling Pathway and Regulates Fungal Development, Virulence, and ER Homeostasis in Magnaporthe oryzae

“…Additionally, the conidial suspension that was adjusted to a concentration of 5 × 10 4 conidia/mL with 0.2% (w/v) gelatin was spray-inoculated on CO-39 seedlings, a susceptible host. The disease severity was assessed as previously reported [65,66]. For the appressorial penetration assays, conidial suspension at a concentration of 5 × 10 4 conidia/mL was dropped on the intact and wounded barley leaves.…”