The platelet‐derived growth factor receptor alpha promoter‐directed expression of cre recombinase in mouse placenta

Wattez, Jean-Sebastien; Qiao, Liang; Lee, Samuel; Natale, David R.C.; Shao, Jianhua

doi:10.1002/dvdy.21

Cited by 11 publications

(10 citation statements)

References 47 publications

(81 reference statements)

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“…To determine the role of ERK signaling in calvarial osteoblast fate decisions, we generated a cranial mesenchyme restricted loss-of-function ERK signaling mouse model. PdgfrαCreER is active broadly across the cranial mesenchyme as early as E9.0 as previously described [ 40 , 46 , 47 ]. We induced CreER recombination in the mouse cranial mesenchyme prior to calvarial osteoblast lineage decisions by administering tamoxifen at E8.5 and E9.5 ( Figure 1 A).…”

Section: Resultsmentioning

confidence: 86%

Wnt-Dependent Activation of ERK Mediates Repression of Chondrocyte Fate during Calvarial Development

Ibarra

Machen

Atit

2021

JDB

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Wnt signaling regulates cell fate decisions in diverse contexts during development, and loss of Wnt signaling in the cranial mesenchyme results in a robust and binary cell fate switch from cranial bone to ectopic cartilage. The Extracellular signal-regulated protein kinase 1 and 2 (ERK1/2) and Wnt signaling pathways are activated during calvarial osteoblast cell fate selection. Here, we test the hypothesis that ERK signaling is a mediator of Wnt-dependent cell fate decisions in the cranial mesenchyme. First, we show that loss of Erk1/2 in the cranial mesenchyme results in a diminished domain of osteoblast marker expression and increased expression of cartilage fate markers and ectopic cartilage formation in the frontal bone primordia. Second, we show that mesenchyme Wnt/β-catenin signaling and Wntless are required for ERK activation in calvarial osteoblasts. Third, we demonstrate that Wnt and ERK signaling pathways function together to repress SOX9 expression in mouse cranial mesenchyme. Our results demonstrate an interaction between the Wnt and ERK signaling pathways in regulating lineage selection in a subset of calvarial cells and provide new insights into Wnt-dependent cell fate decisions.

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Section: Resultsmentioning

confidence: 86%

Wnt-Dependent Activation of ERK Mediates Repression of Chondrocyte Fate during Calvarial Development

Ibarra

Machen

Atit

2021

JDB

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“…Animals. PDGFRα-CreER T2 transgenic mice are crossmated with ACTB-tdTomato, -EGFP reporter mice in order to generate PDGFRα-CreERT2; mT/mG mice [23]. Genotyping is performed as previously described.…”

Section: Methodsmentioning

confidence: 99%

[Retracted] Lineage Contribution of PDGFRα‐Expressing Cells in the Developing Mouse Eye

Zhuo

Diao

et al. 2021

BioMed Research International

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PDGFRα signaling is critically important in ocular development. Previous data on PDGFRα lacks an expression map with high spatial and temporal resolution and lineage information. In this study, we aim to present a detailed PDGFRα expression and lineage map from early embryogenesis to adulthood. PDGFRα-CreER; mT/mG reporter mice were analyzed. mEGFP-positive cells contributed to multiple ocular lineages in a spatiotemporally regulated manner. A dynamic PDGFRα expression was identified in corneal stromal cells, lens epithelial cells, lens fiber cells, and retinal astrocytes during the entire period of eye development, while PDGFRα expression in retinal astrocytes from E17.5 onwards and in Müller glial cells was identified within two weeks after birth. By revealing detailed characterization of gene expression and function, we present a comprehensive map of PDGFRα-expressing cells in the eye for a better understanding of PDGFRα signaling’s role during eye development.

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“…Tables 1–6 list the Cre lines discussed and include references of documents describing Cre activity in bone and off‐target sites, and original articles reporting the lines (knock‐in–based Cre lines in these tables are labeled with an asterisk). ( 22‐213 )…”

Section: Introductionmentioning

confidence: 99%

Sites of Cre-recombinase activity in mouse lines targeting skeletal cells

Couasnay

Madel

Lim

et al. 2020

Journal of Bone and Mineral Research

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The Cre/Lox system is a powerful tool in the biologist's toolbox, allowing loss-of-function and gain-of-function studies, as well as lineage tracing, through gene recombination in a tissue-specific and inducible manner. Evidence indicates, however, that Cre transgenic lines have a far more nuanced and broader pattern of Cre activity than initially thought, exhibiting "off-target" activity in tissues/cells other than the ones they were originally designed to target. With the goal of facilitating the comparison and selection of optimal Cre lines to be used for the study of gene function, we have summarized in a single manuscript the major sites and timing of Cre activity of the main Cre lines available to target bone mesenchymal stem cells, chondrocytes, osteoblasts, osteocytes, tenocytes, and osteoclasts, along with their reported sites of "off-target" Cre activity. We also discuss characteristics, advantages, and limitations of these Cre lines for users to avoid common risks related to overinterpretation or misinterpretation based on the assumption of strict cell-type specificity or unaccounted effect of the Cre transgene or Cre inducers.

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The platelet‐derived growth factor receptor alpha promoter‐directed expression of cre recombinase in mouse placenta

Cited by 11 publications

References 47 publications

Wnt-Dependent Activation of ERK Mediates Repression of Chondrocyte Fate during Calvarial Development

Wnt-Dependent Activation of ERK Mediates Repression of Chondrocyte Fate during Calvarial Development

[Retracted] Lineage Contribution of PDGFRα‐Expressing Cells in the Developing Mouse Eye

Sites of Cre-recombinase activity in mouse lines targeting skeletal cells

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