The pleiotropic effects of the glutamate dehydrogenase (GDH) pathway in Saccharomyces cerevisiae

Mara, Paraskevi; Fragiadakis, George S; Gkountromichos, Fotios; Alexandraki, Despina

doi:10.1186/s12934-018-1018-4

Cited by 36 publications

(34 citation statements)

References 118 publications

(213 reference statements)

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“…Low temperature diminished the concentrations in the wine strain but increased it in the laboratory strain. As mentioned earlier, overexpression of this protein may be detrimental at low temperatures due to a NADH-NAD imbalance 33 . However, a downward shift in the growth temperature also induced an oxidative stress response.…”

Section: Response Of Cenpk Strain Versus Ady5 Strainmentioning

confidence: 82%

“…In addition, isoleucine has been described as part of a set of amino acids typically abundant in thermophilic organisms, contributing to a high thermal stability of proteins 30 . Likewise, Mara and co-authors observed that overexpression of Gdh1 had detrimental effects on yeast growth at 15 ºC 31 . The GDH pathway is involved in the recycling of NADH-NAD by controlling the levels of α -ketoglutarate.…”

Section: Strain-specific Responses To Sub-and Supra-optimal Temperaturementioning

confidence: 89%

“…Gdh1 is involved in glutamate synthesis, which is thought to be part of the defense against oxidative stress through the glutathione system, preventing cold-induced reactive oxygen species (ROS) accumulation 31 . This double and apparently contradictory role of Gdh1 in the cold growth of yeast strains has already been observed by Ballester-Tomás et al (2015) 64 and emphasizes the complexity of its responses.…”

Section: Response Of Cenpk Strain Versus Ady5 Strainmentioning

confidence: 99%

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Differential proteomic analysis by SWATH-MS unravels the most dominant mechanisms underlying yeast adaptation to non-optimal temperatures under anaerobic conditions

Pinheiro

Lip

García‐Ríos

et al. 2020

Preprint

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Elucidation of temperature tolerance mechanisms in yeast is essential for enhancing cellular robustness of strains, providing more economically and sustainable processes. We investigated the differential responses of three distinct Saccharomyces cerevisiae strains, an industrial wine strain, ADY5, a laboratory strain, CEN.PK113-7D and an industrial bioethanol strain, EthanolRed, grown at sub-and supra-optimal temperatures under chemostat conditions. We employed anaerobic conditions, mimicking the industrial processes. The proteomic profile of these strains was performed by SWATH-MS, allowing the quantification of 997 proteins, data available via ProteomeXchange (PXD016567). Our analysis demonstrated that temperature responses differ between the strains; however, we also found some common responsive proteins, revealing that the response to temperature involves general stress and specific mechanisms. Overall, sub-optimal temperature conditions involved a higher remodeling of the proteome. The proteomic data evidenced that the cold response involves strong repression of translation-related proteins as well as induction of amino acid metabolism, together with components related to protein folding and degradation while, the high temperature response mainly recruits amino acid metabolism. Our study provides a global and thorough insight into how growth temperature affects the yeast proteome, which can be a step forward in the comprehension and improvement of yeast thermotolerance.

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Section: Response Of Cenpk Strain Versus Ady5 Strainmentioning

confidence: 82%

Section: Strain-specific Responses To Sub-and Supra-optimal Temperaturementioning

confidence: 89%

Section: Response Of Cenpk Strain Versus Ady5 Strainmentioning

confidence: 99%

See 1 more Smart Citation

Differential proteomic analysis by SWATH-MS unravels the most dominant mechanisms underlying yeast adaptation to non-optimal temperatures under anaerobic conditions

Pinheiro

Lip

García‐Ríos

et al. 2020

Preprint

View full text Add to dashboard Cite

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“…Several reviews exist that focus on yeast nitrogen sensing, signalling, transport, assimilation and metabolism [27][28][29][30][31][32][33][34][35]. A general conclusion is that yeast growth rate is not only associated with the amount of available nitrogen, but also with the quality of the nitrogen source [18,27].…”

Section: Yeast Nitrogen Utilization Under Wine Fermentative Conditionsmentioning

confidence: 99%

Disentangling the genetic bases of Saccharomyces cerevisiae nitrogen consumption and adaptation to low nitrogen environments in wine fermentation

2020

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The budding yeast Saccharomyces cerevisiae has been considered for more than 20 years as a premier model organism for biological sciences, also being the main microorganism used in wide industrial applications, like alcoholic fermentation in the winemaking process. Grape juice is a challenging environment for S. cerevisiae, with nitrogen deficiencies impairing fermentation rate and yeast biomass production, causing stuck or sluggish fermentations, thus generating sizeable economic losses for wine industry. In the present review, we summarize some recent efforts in the search of causative genes that account for yeast adaptation to low nitrogen environments, specially focused in wine fermentation conditions. We start presenting a brief perspective of yeast nitrogen utilization under wine fermentative conditions, highlighting yeast preference for some nitrogen sources above others. Then, we give an outlook of S. cerevisiae genetic diversity studies, paying special attention to efforts in genome sequencing for population structure determination and presenting QTL mapping as a powerful tool for phenotype-genotype correlations. Finally, we do a recapitulation of S. cerevisiae natural diversity related to low nitrogen adaptation, specially showing how different studies have left in evidence the central role of the TORC1 signalling pathway in nitrogen utilization and positioned wild S. cerevisiae strains as a reservoir of beneficial alleles with potential industrial applications (e.g. improvement of industrial yeasts for wine production). More studies focused in disentangling the genetic bases of S. cerevisiae adaptation in wine fermentation will be key to determine the domestication effects over low nitrogen adaptation, as well as to definitely proof that wild S. cerevisiae strains have potential genetic determinants for better adaptation to low nitrogen conditions.

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“…We also observed an activation of the glutamate dehydrogenase (GDH) pathway, indicated by the upregulation of the ammonia permease coding gene YGR121C (MEP1), YOR375C (GDH1) and YOR375C (GDH3), coding for two NADP-dependent GDH isoforms for glutamate synthesis, and the glutamine synthase (GS) gene YPR035W (GLN1), and for the reduced expression of YDL215C (GDH2), another NADP-dependent GDH responsible for glutamate degradation. The GDH pathway is known to be regulated by the quality and availability of nitrogen and carbon sources 77 . Upon exposure to 800 mg L −1 of GN, two of the main regulators of the nitrogen catabolite repression (NCR) pathway, YFL021W (GAT1) and YER040W (GLN3) appear overexpressed as well as two of the main regulators associated to sugar catabolite repression (YGL035C) MIG1 and (YGL035C) GAL80.…”

Section: Scientific Reports |mentioning

confidence: 99%

Toxicological response of the model fungus Saccharomyces cerevisiae to different concentrations of commercial graphene nanoplatelets

Suárez-Diez

Porras

Laguna-Teno

et al. 2020

Sci Rep

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Graphene nanomaterials have attracted a great interest during the last years for different applications, but their possible impact on different biological systems remains unclear. Here, an assessment to understand the toxicity of commercial polycarboxylate functionalized graphene nanoplatelets (Gn) on the unicellular fungal model Saccharomyces cerevisiae was performed. While cell proliferation was not negatively affected even in the presence of 800 mg L −1 of the nanomaterial for 24 hours, oxidative stress was induced at a lower concentration (160 mg L −1), after short exposure periods (2 and 4 hours). no DnA damage was observed under a comet assay analysis under the studied conditions. in addition, to pinpoint the molecular mechanisms behind the early oxidative damage induced by Gn and to identify possible toxicity pathways, the transcriptome of S. cerevisiae exposed to 160 and 800 mg L −1 of Gn was studied. Both GN concentrations induced expression changes in a common group of genes (337), many of them related to the fungal response to reduce the nanoparticles toxicity and to maintain cell homeostasis. Also, a high number of genes were only differentially expressed in the GN800 condition (3254), indicating that high GN concentrations can induce severe changes in the physiological state of the yeast.

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The pleiotropic effects of the glutamate dehydrogenase (GDH) pathway in Saccharomyces cerevisiae

Cited by 36 publications

References 118 publications

Differential proteomic analysis by SWATH-MS unravels the most dominant mechanisms underlying yeast adaptation to non-optimal temperatures under anaerobic conditions

Differential proteomic analysis by SWATH-MS unravels the most dominant mechanisms underlying yeast adaptation to non-optimal temperatures under anaerobic conditions

Disentangling the genetic bases of Saccharomyces cerevisiae nitrogen consumption and adaptation to low nitrogen environments in wine fermentation

Toxicological response of the model fungus Saccharomyces cerevisiae to different concentrations of commercial graphene nanoplatelets

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